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1.4: Significant Figures Mastery Check
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Owls, such as the young snowy owls on the previous page, have for centuries been symbols of both wisdom and mystery. To many cultures their piercing eyes have conveyed a look of intelligence. Their silent flight through darkened landscapes in search of prey has projected an air of power or wonder. For this chapter and this book, owls are an engaging example of a living organism from the world of biology—the study of life. BIOLOGY AND YOU Living in a small town, in the country, or at the edge of the suburbs, one may be lucky enough to hear an owl's hooting. This experience can lead to questions about where the bird lives, what it hunts, and how it finds its prey on dark, moonless nights. Biology, or the study of life, offers an organized and scientific framework for posing and answering such questions about the natural world. Biologists study questions about how living things work, how they interact with the environment, and how they change over time. Biologists study many different kinds of living things ranging from tiny organisms, such as bacteria, to very large organisms, such as elephants. Each day, biologists investigate subjects that affect you and the way you live. For example, biologists determine which foods are healthy. As shown in Figure 1-1, everyone is affected by this impor- tant topic. Biologists also study how much a person should exer- cise and how one can avoid getting sick. Biologists also study what CHARACTERISTICS OF LIFE The world is filled with familiar objects, such as tables, rocks, plants, pets, and automobiles. Which of these objects are living or were once living? What are the criteria for assigning something to the living world or the nonliving world? Biologists have established that living things share seven characteristics of life. These characteristics are organization and the presence of one or more cells, response to a stimulus (plural, stimuli), homeostasis, metabolism, growth and development, reproduction, and change through time. Organization and Cells Organization is the high degree of order within an organism’s internal and external parts and in its interactions with the living world. For example, compare an owl to a rock. The rock has a spe- cific shape, but that shape is usually irregular. Furthermore, differ- ent rocks, even rocks of the same type, are likely to have different shapes and sizes. In contrast, the owl is an amazingly organized individual, as shown in Figure 1-2. Owls of the same species have the same body parts arranged in nearly the same way and interact with the environment in the same way. Copyright © by Holt, Rinehart and Winston. All rights reserved. ORGANISM (Barn Owl) ORGAN (Owl’s Ear) TISSUE (Nervous Tissue Within the Ear) CELL (Nerve Cell) your air, land, and fAll living organisms, whether made up of one cell or many cells, have some degree of organization. A cell is the smallest unit that can perform all life’s processes. Some organisms, such as bacteria, are made up of one cell and are called unicellular (YOON-uh-SEL-yoo-luhr) organisms. Other organisms, such as humans or trees, are made up of multiple cells and are called multicellular (MUHL-ti-SEL-yoo-luhr) organisms. Complex multicellular organisms have the level of orga- nization shown in Figure 1-2. In the highest level, the organism is made up of organ systems, or groups of specialized parts that carry out a certain function in the organism. For example, an owl’s ner- vous system is made up of a brain, sense organs, nerve cells, and other parts that sense and respond to the owl’s surroundings. Organ systems are made up of organs. Organs are structures that carry out specialized jobs within an organ system. An owl’s ear is an organ that allows the owl to hear. All organs are made up of tissues. Tissues are groups of cells that have similar abilities and that allow the organ to function. For example, nervous tissue in the ear allows the ear to detect sound. Tissues are made up of cells. A cell must be covered by a membrane, contain all genetic information necessary for replication, and be able to carry out all cell functions. Within each cell are organelles. Organelles are tiny structures that carry out functions necessary for the cell to stay alive. Organelles contain biological molecules, the chemical compounds that provide physical structure and that bring about movement, energy use, and other cellular functions. All biological molecules are made up of atoms. Atoms are the simplest particle of an ele- ment that retains all the properties of a certain element. Response to Stimuli Another characteristic of life is that an organism can respond to a stimulus—a physical or chemical change in the internal or external environment. For example, an owl dilates its pupils to keep the level of light entering the eye constant. Organisms must be able to respond and react to changes in their environment to stay alive. ORGANELLE (Mitochondrion) BIOLOGICAL MOLECULE (Phospholipid) ATOM (Oxygen) cell from the Latin, cella meaning “small room,” or “hut” Word Roots and Origins www.scilinks.org Topic: Characteristics of Life Keyword: HM60257 mb06se_bios01.qxd 5/18/07 10:37 AM Page 7 8 CHAPTER 1 Homeostasis All living things, from single cells to entire organisms, have mecha- nisms that allow them to maintain stable internal conditions. Without these mechanisms, organisms can die. For example, a cell’s water content is closely controlled by the taking in or releas- ing of water. A cell that takes in too much water will rupture and die. A cell that doesn’t get enough water will also shrivel and die. Homeostasis (HOH-mee-OH-STAY-sis) is the maintenance of a stable level of internal conditions even though environmental conditions are constantly changing. Organisms have regulatory systems that maintain internal conditions, such as temperature, water content, and uptake of nutrients by the cell. In fact, multi- cellular organisms usually have more than one way of maintain- ing important aspects of their internal environment. For example, an owl’s temperature is maintained at about 40°C (104°F). To keep a constant temperature, an owl’s cells burn fuel to produce body heat. In addition, an owl’s feathers can fluff up in cold weather. In this way, they trap an insulating layer of air next to the bird’s body to maintain its body temperature. Metabolism Living organisms use energy to power all the life processes, such as repair, movement, and growth. This energy use depends on metabolism (muh-TAB-uh-LIZ-uhm). Metabolism is the sum of all the chemical reactions that take in and transform energy and materials from the environment. For example, plants, algae, and some bacteria use the sun’s energy to generate sugar molecules during a process called photosynthesis. Some organisms depend on obtaining food energy from other organisms. For instance, an owl’s metabolism allows the owl to extract and modify the chemi- cals trapped in its nightly prey and use them as energy to fuel activities and growth. Growth and Development All living things grow and increase in size. Some nonliving things, such as crystals or icicles, grow by accumulating more of the same material of which they are made. In contrast, the growth of living things results from the division and enlargement of cells. Cell division is the formation of two new cells from an existing cell, as shown in Figure 1-3. In unicellular organisms, the primary change that occurs following cell division is cell enlargement. In multi- cellular life, however, organisms mature through cell division, cell enlargement, and development. Development is the process by which an organism becomes a mature adult. Development involves cell division and cell differen- tiation, or specialization. As a result of development, an adult organism is composed of many cells specialized for different func- tions, such as carrying oxygen in the blood or hearing. In fact, the human body is composed of trillions of specialized cells, all of which originated from a single cell, the fertilized egg. This unicellular organism, Escherichia coli, inhabits the human intestines. E. coli reproduces by means of cell division, during which the original cell splits into two identical offspring cells. FIGURE 1-3 Observing Homeostasis Materials 500 mL beakers (3), wax pen, tap water, thermometer, ice, hot water, goldfish, small dip net, watch or clock with a second hand Procedure 1. Use a wax pen to label three 500 mL beakers as follows: 27°C (80°F), 20°C (68°F), 10°C (50°F). Put 250 mL of tap water in each beaker. Use hot water or ice to adjust the tem- perature of the water in each beaker to match the temperature on the label. 2. Put the goldfish in the beaker of 27°C water. Record the number of times the gills move in 1 minute. 3. Move the goldfish to the beaker of 20°C water. Repeat observations. Move the goldfish to the beaker of 10°C. Repeat observations. Analysis What happens to the rate at which gills move when the temp- erature changes? Why? How do gills help fish maintain homeostasis? Quick Lab mb06se_bios01.qxd 5/18/07 10:37 AM Page 8 THE SCIENCE OF LIFE 9 Reproduction All organisms produce new organisms like themselves in a process called reproduction. Reproduction, unlike other characteristics, is not essential to the survival of an individual organism. However, because no organism lives forever, reproduction is essential for the continuation of a species. Glass frogs, as shown in Figure 1-4, lay many eggs in their lifetime. However, only a few of the frogs’ off- spring reach adulthood and successfully reproduce. During reproduction, organisms transmit hereditary informa- tion to their offspring. Hereditary information is encoded in a large molecule called deoxyribonucleic acid, or DNA. A short segment of DNA that contains the instructions for a single trait of an organism is called a gene. DNA is like a large library. It contains all the books—genes—that the cell will ever need for making all the struc- tures and chemicals necessary for life. Hereditary information is transferred to offspring during two kinds of reproduction. In sexual reproduction, hereditary information recombines from two organisms of the same species. The resulting offspring are similar but not identical to their parents. For example, a male frog’s sperm can fertilize a female’s egg and form a single fer- tilized egg cell. The fertilized egg then develops into a new frog. In asexual reproduction, hereditary information from different organisms is not combined; thus the original organism and the new organism are genetically the same. A bacterium, for example, reproduces asexually when it splits into two identical cells. Change Through Time Although individual organisms experience many changes during their lifetime, their basic genetic characteristics do not change. However, populations of living organisms evolve or change through time. The ability of populations of organisms to change over time is important for survival in a changing world. This factor is also impor- tant in explaining the diversity of life-forms we see on Earth today. 1. How does biology affect a person’s daily life? 2. How does biology affect society? 3. Name the characteristics shared by living things. 4. Summarize the hierarchy of organization found in complex multicellular organisms. 5. What are the different functions of homeostasis and metabolism in living organisms? 6. How does the growth among living and nonliv- ing things differ? 7. Why is reproduction an important characteristic of life? CRITICAL THINKING 8. Applying Information Crystals of salt grow and are highly organized. Why don’t biologists con- sider them to be alive? 9. Analyzing Models When a scientist designs a space probe to detect life on a distant planet, what kinds of things should it measure? 10. Making Comparisons Both cells and organisms share the characteristics of life. How are cells and organismsood supply will be like in the near future.EVOLUTION OF LIFE Individual organisms change during their lifetime, but their basic genetic characteristics do not change. However, populations of liv- ing organisms do change through time, or evolve. Evolution, or descent with modification, is the process in which the inherited characteristics within populations change over generations, such that genetically distinct populations and new species can develop. Evolution as a theme in biology helps us understand how the various branches of the “tree of life” came into existence and have changed over time. It also explains how organisms alive today are related to those that lived in the past. Finally, it helps us understand the mechanisms that underlie the way organisms look and behave. Natural Selection The ability of populations of organisms to change over time is important for survival in a changing world. According to the theory of evolution by natural selection, organisms that have certain favorable traits are better able to survive and reproduce success- fully than organisms that lack these traits. One product of natural selection is the adaptation of organisms to their environment. Adaptations are traits that improve an indi- vidual’s ability to survive and reproduce. For example, rabbits with white fur and short ears in a snowy place, such as the one in Figure 1-7a, may avoid predators and frostbitten ears more often than those with dark fur and long ears. Thus, the next generation of rabbits will have a greater percentage of animals carrying the genes for white fur and short ears. In contrast, the brown, long- eared rabbit, as shown in Figure 1-7b, would survive and reproduce more successfully in a hot desert environment. The survival and reproductive success of organisms with favor- able traits cause a change in populations of organisms over gener- ations. This descent with modification is an important factor in explaining the diversity of organisms we see on Earth today. 1. Name three unifying themes found in biology. 2. How is the unity and diversity in the living world represented? 3. Identify the three domains and the kingdoms found in each domain. 4. How are organisms interdependent? 5. Describe why evolution is important in explain- ing the diversity of life. 6. Distinguish between evolution and natural selection. CRITICAL THINKING 7. Applying Information Assign the various top- pings you put on pizza to the appropriate domains and kingdoms of life. 8. Analyzing Graphics According to the “tree” in Figure 1-5, which of these pairs are more closely related: Archaea:Bacteria or Archaea:Eukarya? 9. Making Hypotheses Fossil evidence shows that bats descended from shrewlike organisms that could not fly. Write a hypothesis for how natural selection might have led to flying bats. SECTION 2 REVIEW (a) This short-eared arctic hare, Lepus arcticus, is hidden from predators and protected from frostbite in a snowy environment. (b) The mottled brown coats of desert rabbits blend in with the dirt and dry grasses, and their long ears help them radiate excess heat and thus avoid overheating. FIGURE 1-7 (a) (b) Copyright © by Holt, Rinehart and Winston. All rights reserved. THE SCIENCE OF LIFE 13 TH E STUDY OF BIOLOGY Curiosity leads us to ask questions about life. Science provides a way of answering such questions about the natural world. Science is a systematic method that involves forming and testing hypotheses. More importantly, science relies on evidence, not beliefs, for drawing conclusions. SCIENCE AS A PROCESS Science is characterized by an organized approach, called the scientific method, to learn how the natural world works. The methods of science are based on two important principles. The first principle is that events in the natural world have natural causes. For example, the ancient Greeks believed that lightning and thunder occurred because a supernatural god Zeus hurled thunderbolts from the heavens. By contrast, a scientist considers lightning and thunder to result from electric charges in the atmos- phere. When trying to solve a puzzle from nature, all scientists, such as the one in Figure 1-8, accept that there is a natural cause to solve that puzzle. A second principle of science is uniformity. Uniformity is the idea that the fundamental laws of nature operate the same way at all places and at all times. For example, scientists assume that the law of gravity works the same way on Mars as it does on Earth. Steps of the Scientific Method Although there is no single method for doing science, scientific studies involve a series of common steps. 1. The process of science begins with an observation. An observation is the act of perceiving a natural occurrence that causes someone to pose a question. 2. One tries to answer the question by forming hypotheses (singular, hypothesis). A hypothesis is a proposed explanation for the way a particular aspect of the natural world functions. 3. A prediction is a statement that forecasts what would happen in a test situation if the hypothesis were true. A prediction is recorded for each hypothesis. 4. An experiment is used to test a hypothesis and its predictions. 5. Once the experiment has been concluded, the data are analyzed and used to draw conclusions. 6. After the data have been analyzed, the data and conclusions are communicated to scientific peers and to the public. This way oth- ers can verify, reject, or modify the researcher’s conclusions. SECTION 3 OBJECTIVES ● Outline the main steps in the scientific method. ● Summarize how observations are used to form hypotheses. ● List the elements of a controlled experiment. ● Describe how scientists use data to draw conclusions. ● Compare a scientific hypothesis and a scientific theory. ● State how communication in science helps prevent dishonesty and bias. VOCABULARY scientific method observation hypothesis prediction experiment control group experimental group independent variable dependent variable theory peer review All researchers, such as the one releasing an owl above, use the scientific method to answer the questions they have about nature. FIGURE 1-8 Copyright © by Holt, Rinehart and Winston. All rights reserved. 14 CHAPTER 1 OBSERVING AND ASKING QUESTIONS The scientific method generally begins with an unexplained observa- tion about nature. For example, people have noticed for thousands of years that owls can catch prey in near total darkness. As shown in steps and of Figure 1-9, an observation may then raise ques- tions. The owl observation raises the question: How does an owl detect prey in the dark? FORMING A HYPOTHESIS After stating a question, a biologist lists possible answers to a sci- entific question—hypotheses. Good hypotheses answer a question and are testable in the natural world. For example, as shown in step Figure 1-9, there are several possible hypotheses for the question of how owls hunt at night: (a) owls hunt by keen vision in the dark; (b) owls hunt by superb hearing; or (c) owls hunt by detecting the prey’s body heat. Predicting To test a hypothesis, scientists make a prediction that logically fol- lows from the hypothesis. A prediction is what is expected to hap- pen if each hypothesis were true. For example, if hypothesis (a) is true, (owls hunt by keen night vision) then one can predict that the owl will pounce only on the mouse in either a light or a dark room. If hypothesis (b) is true (owls hunt by hearing), then one can pre- dict that in a lighted room, the owl will pounce closer to the mouse’s head. But, in a dark room, the owl should pounce closer to a rustling leaf attached to the mouse. Finally, if hypothesis (c) is true (owls hunt by sensing body heat), then an owl would strike only the prey no matter the room conditions, because owls hunt by detecting the prey’s body heat. 3 1 2 Copyright © by Holt, Rinehart and Winston. All rights reserved. A scientific study includes observations, questions, hypotheses, predictions, experiments, data analysis, and conclu- sions. A biologist can use the scientific method to set up an experiment to learn how an owl captures prey at night. FIGURE 1-9 1 OBSERVATION Owls capture prey on dark nights. 2 QUESTION How do owls detect prey on dark nights? 3 HYPOTHESES a) Owls hunt in the dark by vision. b) Owls hunt in the dark by hearing. c) Owls hunt in the dark by sensing body heat. THE SCIENCE OF LIFE 15 Notice that these predictions make it difficult to distinguish be- tween the vision and body heat hypotheses. The reason is that both hypotheses predict that the owl could grab the mouse in a dark room. Also, these three hypotheses do not eliminate all other factors that could influence how the owl finds its prey. However, testing predictions can allow one to begin rejecting hypotheses and thus to get closer to determining the answer(s) to a question. DESIGNING AN EXPERIMENT Biologists often test hypotheses by setting up an experiment. Step in Figure 1-9 outlines an experiment to test the hypotheses about how an owl hunts at night. First, experimenters set up a room with an owl perch high on one side and a small trap door on the other side for releasing mice. Then, they tied a leaf to each mouse’s tail with a string and released each mouse into the room. Next, each mouse ran silently across the room, but the leaf trailed behind, making a rustling noise. During half of the trials, the lights were on. During the other half, the room was dark. Technicians videotaped all the action in the chamber with an infrared light, which owls cannot see. The researchers then viewed the videos and measured the position of the owl’s strike relative to each mouse’s head. Performing the Experiment Many scientists use a controlled experiment to test their hypotheses. A controlled experiment compares an experimental group and a control group and only has one variable. The control group pro- vides a normal standard against which the biologist can compare results of the experimental group. The experimental group is iden- tical to the control group except for one factor, the independent variable. The experimenter manipulates the independent variable, sometimes called the manipulated variable. 4 4 EXPERIMENT 5 DATA COLLECTION AND ANALYSIS Measure and compare the distance from the owl’s strike to the mouse and to the leaf in light and dark. 6 CONCLUSION Data supported the hearing hypothesis: Owls hunt in the dark by hearing. prey Test predictions of the three hypotheses. Control: In the light Experimental: In the dark 1 2 3 4 5 6 7 8 9 10 11 Predicting Results Materials 2 Petri dishes with agar, cellophane tape, wax pen Procedure 1. Open one of the Petri dishes, and streak your finger across the surface of the agar. 2. Replace the lid, and seal it with the tape. Label this Petri dish with your name and a number 1. 3. Seal the second Petri dish with- out removing the lid. Label this Petri dish with your name and the number 2. 4. Write a prediction about what will happen in each dish. Store your dishes as your teacher directs. Record your observations. Follow your teacher’s directions for disposal of your dishes. Analysis Was your prediction accurate? What evidence can you cite to support your prediction? If you did not obtain the results you predicted, would you change your testing method or your prediction? Explain. Evaluate the importance of obtaining a result that does not support your prediction. Quick Lab mb06se_bios03.qxd 5/18/07 10:40 AM Page 15 16 CHAPTER 1 The independent variable in the owl experiment is the presence or absence of light. In the owl experiment, the control group hunts in the light, and the experimental group hunts in the dark. In addi- tion to varying the independent variable, a scientist observes or measures another factor called the dependent variable, or respond- ing variable, because it is affected by the independent variable. In the owl experiment, the dependent variable is distance from the owl’s strike to the mouse’s head. Testing the Experiment Some controlled experiments are conducted “blind.” In other words, the biologist who scores the results is unaware of whether a given subject is part of the experimental or control group. This factor helps eliminate experimenter bias. Experiments should also be repeated, because living systems are variable. Moreover, scien- tists must collect enough data to find meaningful results. COLLECTING AND ANALYZING DATA Most experiments measure a variable—the dependent variable. This measurement provides quantitative data, data measured in numbers. For example, in the experiment above, scientists mea- sured the distance of an owl’s strike from the prey’s head in cen- timeters, as shown in step of Figure 1-9. An event’s duration in milliseconds is also an example of quantitative data. Biologists usually score the results of an experiment by using one of their senses. They might see or hear the results of an experiment. Scientists also extend their senses with a micro- scope for tiny objects or a microphone for soft sounds. In the owl experiment, biologists extended their vision with infrared cameras. Analyzing and Comparing Data After collecting data from a field study or an experiment and then organizing it, biologists then analyze the data. In analyzing data, the goal is to determine whether the data are reliable, and whether they support or fail to support the predictions of the hypothesis. To do so, scientists may use statistics to help determine relation- ships between the variables involved. They can then compare their data with other data that were obtained in other similar studies. It is also important at this time to determine possible sources of error in the experiment just per- formed. Scientists usually display their data in tables or graphs when analyzing it. For the owl study, biologists could have made a bar graph such as the one in Figure 1-10, which shows the average distance from the owl’s strike relative to the mouse’s head or the leaf in the light and in the dark. 5 5 0 10 15 20 25 In the light In the dark Average distance from strike (cm) Distance Between Owl Strike and a Mouse or From a Leaf Attached to Mouse 30 Mouse Leaf Mouse Leaf The data below are hypothetical results that might occur from the described owl experiment.The independent variable is the darkness of the room, and the dependent variable is how far the owl struck from the mouse’s head.The data show that the owl strikes more accurately at the mouse in the light but strikes more accurately at the leaf in the dark. FIGURE 1-10 Copyright © by Holt, Rinehart and Winston. All rights reserved. THE SCIENCE OF LIFE 17 DRAWING CONCLUSIONS Biologists analyze their tables, graphs, and charts to draw conclu- sions about whether or not a hypothesis is supported, as shown in step of Figure 1-9. The hypothetical owl data show that in the light, owls struck with greater accuracy at the mouse than at the leaf, but in the dark, owls struck with greater accuracy at the leaf than the mouse. Thus, the findings support the hearing hypothe- sis, but not the vision hypothesis. An experiment can only disprove, not prove, a hypothesis. For example, one cannot conclude from the results that the hearing hypothesis is proven to be true. Perhaps the owl uses an unknown smell to strike at the mouse. One can only reject the vision hypothe- sis because it did not predict the results of the experiment correctly. Acceptance of a hypothesis is always tentative in science. The scientific community revises its understanding of phenomena, based on new data. Having ruled out one hypothesis, a biologist will devise more tests to try to rule out any remaining hypotheses. Making Inferences Scientists often draw inferences from data gathered during a field study or experiment. An inference (IN-fuhr-uhns) is a conclusion made on the basis of facts and previous knowledge rather than on direct observations. Unlike a hypothesis, an inference is not directly testable. In the owl study, it is inferred that the owl detects prey from a distance rather than by direct touch. Applying Results and Building Models As shown in Figure 1-11, scientists often apply their findings to solve practical problems. They also build models to represent or describe things. For example in 1953, James Watson and Francis Crick used cardboard balls and wire bars to build physical models of atoms in an attempt to understand the structure of DNA. Mathematical models are sets of equations that describe how dif- ferent measurable items interact in a system. The experimenter can adjust variables to better model the real-world data. CONSTRUCTING A THEORY When a set of related hypotheses is confirmed to be true many times, and it can explain a great amount of data, scientists often reclassify it as a theory. Some examples include the quantum the- ory, the cell theory, or the theory of evolution. People commonly use the word “theory” in a different way than scientists use the word. People may say “It’s just a theory” suggesting that an idea is untested, but scientists view a theory as a highly tested, generally accepted principle that explains a vast number of observations and experimental data. 6 Copyright © by Holt, Rinehart and Winston. All rights reserved. Biologists often apply their knowledge of the natural world to practical problems. Studies on the owl’s keen ability to locate sounds in space despite background noise are helping biotechnologists and bioengineers develop better solutions for people with impaired hearing, such as the people shown in this picture. FIGURE 1-11 18 CHAPTER 1 COMMUNICATING IDEAS An essential aspect of scientific research is scientists working together. Scientists often work together in research teams or sim- ply share research results with other scientists. This is done by publishing findings in scientific journals or presenting them at sci- entific meetings, as shown in Figure 1-12. Sharing information allows others working independently to verify findings or to con- tinue work on established results. For example, Roger Payne pub- lished the results of his owl experiments in a journal in 1971. Then, other biologists could repeat it for verification or use it to study the mechanisms introduced by the paper. With the growing impor- tance of science in solving societal issues, it is becoming increas- ingly vital for scientists to be able to communicate with the public at large. Publishing a Paper Scientists submit research papers to scientific journals for publica- tion. A typical research paper has four sections. First, the Introduction poses the problem and hypotheses to be investigated. Next, the Materials and Methods describe how researchers proceeded with the experiment. Third, the Results state the findings the experiment presented, and finally, the Discussion gives the significance of the experiment and future directions the scientists will take. Job Description Forensic biolo- gists are scientists who study biological materials to investigate potential crimes and other legal issues against humans and animals. Forensic scientists have knowledge in areas of biology, such as DNA and blood pattern analysis, and work in private sector and public laboratories. Focus On a Forensic Biologist As a law enforcement forensic specialist for the Texas Parks and Wildlife Department, Beverly Villarreal assists the game warden in investigations of fish and wildlife violations, such as illegal hunting and fishing. Villarreal analyzes blood and tissue samples to identify species of animals such as fish, birds, and reptiles. Her work helps game wardens as they enforce state laws regarding hunting and fishing. Most people think of forensic scientists as the glamorous crime investigators on TV, but according to Villarreal real forensic scientists “spend a great deal of time at a lab bench running analysis after analysis.” Many of the methods used in animal forensics, such as DNA sequenc- ing, are also used in human forensics. Education and Skills • High school—three years of science courses and four years of math courses. • College—bachelor of science in biol- ogy, including course work in zoology and genetics, plus experience in per- forming DNA analyses. • Skills—patience, attention to detail, and ability to use fine tools. Careers in BIOLOGY Forensic Biologist For more about careers, visit go.hrw.com and type in the keyword HM6 Careers. www.scilinks.org Topic: Scientific Investigations Keyword: HM61358 mb06se_bios03.qxd 5/18/07 10:40 AM Page 18 THE SCIENCE OF LIFE 19 1. What two principles make the scientific method a unique process? 2. Define the roles of observations and hypotheses in science. 3. Summarize the parts of a controlled experiment. 4. Summarize how we make conclusions about the results of an experiment. 5. Why is the phrase, “it’s just a theory” misleading? 6. Give another example of a conflict of interest. CRITICAL THINKING 7. Making Hypotheses On a nocturnal owl’s skull, one ear points up, and the other ear points down. Suggest a hypothesis for this observation. 8. Designing Experiments Design an experiment to establish if owls hunt by keen sight or hunt by heat seeking. 9. Calculating Information What was the average distance between the owl’s strike and the mouse if the recorded differences in this experiment were 25, 22, 19, 19, and 15? SECTION 3 REVIEW After scientists submit their papers to a scientific journal, the editors of that journal will send the paper out for peer review. In a peer review, scientists who are experts in the field anonymously read and critique that research paper. They determine if a paper pro- vides enough information so that the experiment can be duplicated and if the author used good experimental controls and reached an accurate conclusion. They also check if the paper is written clearly enough for broad understanding. Careful analysis of each other’s research by fellow scientists is essential to making scientific progress and preventing scientific dishonesty. HONESTY AND BIAS The scientific community depends on both honesty and good sci- ence. While designing new studies, experimenters must be very careful to prevent previous ideas and biases from tainting both the experimental process and the conclusions. Scientists have to keep in mind that they are always trying to disprove their favorite ideas. Scientists repeat experiments to verify previous findings. This allows for science to have a method for self-correction and it also keeps researchers honest and credible to their peers in the field. Conflict of Interest For most scientists, maintaining a good reputation for collecting and presenting valid data is more important than temporary prestige or income. So, scientists try to avoid any potential conflicts of interest. For example, a scientist who owns a biotechnology company and manufactures a drug would not be the best researcher to critically test that drug’s safety and effectiveness. To avoid this potential con- flict of interest, the scientist allows an unaffected party, such as a research group, to test the drug’s effectiveness. The threat of a potential scandal based on misleading data or conclusions is a pow- erful force in science that helps keep scientists honest and fair. Scientists present their experiments in various forms. The scientists above are presenting their work in the form of a poster at a scientific meeting. FIGURE 1-12 Copyright © by Holt, Rinehart and Winston. All rights reserved. The Internet can provide a wealth of scientific information for a report, but the information may not always be credible or accurate. You can use the methods above to check the accuracy and credibility of your sources. SCIENCE TECHNOLOGY SOCIETY SCIENCE ON THE INTERNET: A New Information Age I n the past, students research- ing a science topic would typ- ically begin their research by visiting a library to use printed reference materials, such as encyclopedias. Today, most stu- dents research topics by using a computer and searching for information on the Internet. The Internet can provide students with a wealth of infor- mation. But which Web sites have accurate information, and which Web sites do not? Checking Web Addresses Students should use the Web address, or URL, to establish the Web site’s credibility. Usually, the domain name can suggest who has published the Web site. Web sites can be pub- lished by governmental agen- cies (ends in “dot gov” or .gov), by educational institutions (ends in “dot edu” or .edu), by organizations (ends in “dot org” or .org), or by commercial businesses (ends in “dot com” or .com). Government Web sites are usually reliable. Examples of credible governmental Web sites are the National Institutes of Health (NIH) and the Food and Drug Administration (FDA). University and medical school sites are also reliable sources of information. Many organiza- tions that research and teach the public about specific diseases and conditions can also provide reliable information. Examples of such organizations are the American Cancer Society and the American Heart Association. Evaluating Web Sites The credibility of the author of the Web site should also be checked. Make sure the author is not trying to sell anything and is established in his or her field. For example, a health Web site’s author should be a med- ical professional. It is also important to check the date that the information was posted on the Web to ensure that the information is current. Also, the Web site should provide ref- erences from valid sources, such as scientific journals or govern- ment publications. Finally, the student should always double-check informa- tion between several reliable Web sites. If two or three reliable sites provide the same informa- tion, the student can feel confi- dent in using that information. Web Sites for Students The Internet Connect boxes in this textbook have all been reviewed by professionals at the National Science Teachers Association (NSTA). Students can trust that these sites are reliable sources for science- or health-related topics. REVIEW 1. Which types of Web addresses are the most reliable? 2. List four important features to evaluate when using a Web site for research. 3. Supporting Reasoned Opinions Why do you think a Web site that is advertising a product may not offer accurate information? REVIEW 20 www.scilinks.org Topic: Using the Internet Keyword: HM61589 mb06se_biosts.qxd 5/18/07 10:42 AM Page 20 TOOLS AND TECHNIQUES With proper equipment and good methods, biologists can see, manipulate, and understand the natural world in new ways. Microscopes are one of many useful tools used to unlock nature’s biological secrets. MICROSCOPES AS TOOLS Tools are objects used to improve the performance of a task. Microscopes are tools that extend human vision by making enlarged images of objects. Biologists use microscopes to study organisms, cells, cell parts, and molecules. Microscopes reveal details that otherwise might be difficult or impossible to see. Light Microscopes To see small organisms and cells, biologists typically use a light microscope, such as the one shown in Figure 1-13. A compound light microscope is a microscope that shines light through a spec- imen and has two lenses to magnify an image. To use this micro- scope, one first mounts the specimen to be viewed on a glass slide. The specimen must be thin enough for light to pass through it. For tiny pond organisms, such as the single-celled paramecium, light passing through the organism is not a problem. For thick objects, such as plant stems, biologists must cut thin slices for viewing. There are four major parts of a compound light microscope. For further description of the parts of a micro- scope, see the Appendix. 1. Eyepiece The eyepiece (ocular (AHK-yoo-luhr) lens) magnifies the image, usually 10 times. 2. Objective Lens Light passes through the specimen and then through the objective lens, which is located directly above the specimen. The objective lens enlarges the image of the specimen. Scientists sometimes use stains to make the image easier to see. 3. Stage The stage is a platform that supports a slide holding the specimen. The slide is placed over the opening in the stage of the microscope. 4. Light Source The light source is a light bulb that provides light for viewing the image. It can be either light reflected with a mirror or an incandescent light from a small lamp. SECTION 4 OBJECTIVES ● List the function of each of the major parts of a compound light microscope. ● Compare two kinds of electron microscopes. ● Describe the importance of having the SI system of measurement. ● State some examples of good laboratory practice. VOCABULARY compound light microscope eyepiece (ocular lens) objective lens stage light source magnification nosepiece resolution scanning electron microscope transmission electron microscope metric system base unit Compound light microscopes open the human eye to an interesting world including tiny pond organisms, healthy and diseased cells, and the functioning of cell parts. FIGURE 1-13 Objective lens Eyepiece (ocular lens) Stage Light THE SCIENCE OF LIFE 21 Copyright © by Holt, Rinehart and Winston. All rights reserved. 22 CHAPTER 1 Magnification and Resolution Microscopes vary in powers of magnification and resolution. Magnification is the increase of an object’s apparent size. Revolving the nosepiece, the structure that holds the set of objective lens, rotates these lenses into place above the specimen. In a typical com- pound light microscope, the most powerful objective lens produces an image up to 100 times (100) the specimen’s actual size. The degree of enlargement is called the power of magnification of the lens. The standard ocular lens magnifies a specimen 10 times (10). To compute the power of magnification of a microscope, the power of magnification of the strongest objective lens (in this case, 100) is multiplied by the power of magnification of the ocular lens (10). The result is a total power of magnification of 1000. Resolution (REZ-uh-LOO-shuhn) is the power to show details clearly in an image. The physical properties of light limit the ability of light microscopes to resolve images, as shown in Figure 1-14a. At pow- ers of magnification beyond about 2,000, the image of the speci- men becomes fuzzy. For this reason, scientists use other microscopes to view very small cellsIons Ions are charged substances that have formed through the gain or loss of electrons. Cations form from the loss of electrons and have a positive charge while anions form through the gain of electrons and have a negative charge. Cation Formation Cations are the positive ions formed by the loss of one or more electrons. The most commonly formed cations of the representative elements are those that involve the loss of all of the valence electrons. Consider the alkali metal sodium (Na) . It has one valence electron in the n=3 energy level. Upon losing that electron, the sodiu ion now has an octet of electrons from the second energy level and a charge of 1+ . The electron arrangement of the sodium ion is now the same as that of the noble gas neon. Consider a similar process with magnesium and aluminum. In this case, the magnesium atom loses its two valence electrons in order to achieve the same arrangement as the noble gas neon and a charge of 2+ . The aluminum atom loses its three valence electrons to have the same electron arrangement as neon and a charge of 3+ . For representative elements under typical conditions, three electrons is usually the maximum number that will be los. Representative elements will not lose electrons beyond their valence because they would have to "break" the octet of the previous energy level which provides stability to the ion. Anions Anions are the negative ions formed from the gain of one or more electrons. When nonmetal atoms gain elections, they often do so until their outermost principal energy level achieves an octet. For fluorine, which has an electron arrangement of (2, 7), it only needs to gain one electron to have the same electron arrangement as neon. Forming an octet (eight electrons in the outer shell) provides stability to the atom. Fluorine will gain one electron and have a charge of 1− . The electron arrangement of the fluoride ion (2, 8) will also change to reflect the gain of an electron. Oxygen has an electron arrangement of (2, 6) and needs to gain two electrons to fill the n=2 energy level and achieve an octet of electrons in the outermost shell. The oxide ion will have a charge of 2− as a result of gaining two electrons. Under typical conditions, three electrons is the maximum that will be gained in the formation of anions. Subatomic Particles in an Ion Since ions form from the gain or loss of electrons, we can also look at the number of subatomic particles (protons, neutrons, and electrons) found in an ion. Remember that the number of protons determines the identity of the element and will not change in a chemical process. Example 2.5.1 How many protons, neutrons, and electrons in a single oxide (O2−) ion? Solution Oxygen has the atomic number 8 so both the atom and the ion will have 8 protons. The average atomic mass of oxygen is 16. Therefore, there will be 8 neutrons (atomic mass−atomic number=neutrons) . A neutral oxygen atom would have 8 electrons. However, the anion has gained two electrons so O2− has 10 electrons. We can also use information about the subatomic particles to determine the identity of an ion. Example 2.5.2 An ion with a 2+ charge has 18 electrons. Determine the identity of the ion. Solution If an ion has a 2+ charge then it must have lost electrons to form the cation. If the ion has 18 electrons and the atom lost 2 to form the ion, then the neutral atom contained 20 electrons. Since it was neutral, it must also have had 20 protons. Therefore the element is calcium. Polyatomic Ions A polyatomic ion is an ion composed of two or more atoms that have a charge as a group (poly = many). The ammonium ion (see figure below) consists of one nitrogen atom and four hydrogen atoms. Together, they comprise a single ion with a 1+ charge and a formula of NH+4 . The hydroxide ion (see figure below) contains one hydrogen atom and one oxygen atom with an overall charge of 1− . The carbonate ion (see figure below) consists of one carbon atom and three oxygen atoms and carries an overall charge of 2− . The formula of the carbonate ion is CO2−3 . The atoms of a polyatomic ion are tightly bonded together and so the entire ion behaves as a single unit. The figures below show several examples. Soult Screenshot 2-2-1.png Figure 2.5.1 : The ammonium ion (NH+4) is a nitrogen atom (blue) bonded to four hydrogen atoms (white). Soult Screenshot 2-2-2.png Figure 2.5.2 : The hydroxide ion (OH−) is an oxygen atom (red) bonded to a hydrogen atom. Soult Screenshot 2-2-3.png Figure 2.5.3 : The carbonate ion (CO2−3) is a carbon atom (black) bonded to three oxygen atoms. The table below lists a number of polyatomic ions by name and by structure. The heading for each column indicates the charge on the polyatomic ions in that group. Note that the vast majority of the ions listed are anions - there are very few polyatomic cations. 1− 2− 3− 1+ Table 2.5.1 : Common Polyatomic Ions acetate, CH3COO− carbonate, CO2−3 arsenate, AsO3−3 ammonium, NH+4 bromate, BrO−3 chromate, CrO2−4 phosphite, PO3−3 chlorate, ClO−3 dichromate, Cr2O2−7 phosphate, PO3−4 chlorite, ClO−2 hydrogen phosphate, HPO2−4 cyanide, CN− oxalate, C2O2−4 dihydrogen phosphate, H2PO−4 peroxide, O2−2 hydrogen carbonate, HCO−3 silicate, SiO2−3 hydrogen sulfate, HSO−4 sulfate, SO2−4 hydrogen sulfide, HS− sulfite, SO2−3 hydroxide, OH− hypochlorite, ClO− nitrate, NO−3 nitrite, NO−2 perchlorate, ClO−4 permanganate, MnO−4 The vast majority of polyatomic ions are anions, many of which end in -ate or -ite. Notice that in some cases such as nitrate (NO−3) and nitrite (NO−2) , there are multiple anions that consist of the same two elements. In these cases, the difference between the ions is the number of oxygen atoms present, while the overall charge is the same. As a class, these are called oxyanions. When there are two oxyanions for a particular element, the one with the greater number of oxygen atoms gets the -ate suffix, while the one with the fewer number of oxygen atoms gets the -ite suffix. The four oxyanions of chlorine are shown below, which also includes the use of the prefixes hypo- and per-. ClO− , hypochlorite ClO−2 , chlorite ClO−3 , chlorate ClO−4 , perchlorate Not your usual ion Soult Screenshot 2-2-4.png "Drink you milk. It's good for your bones." We're told this from early childhood, and with good reason. Milk contains a good supply of calcium, part of the structure of bone. However, there are two other ionic components of hydroxyapatite, the mineral component. Phosphate ion and hydroxide ion make up the remainder of the inorganic material in bone. News You Can Use Bone is a very complex structure. It is composed of protein (mainly collagen), hydroxyapatite (a calcium-phosphate-hydroxide mixture), some other minerals, and contains 10 - 20% water. The calcium/phosphate ratios are not stoichiometric, but vary somewhat from one portion of bone to the next. Bones are very strong but will break under enough stress. Regular exercise and proper nutrition help to increase bone strength. Watch a video about bone structure at http://www.youtube.com/watch?v=d9owEvYdouk Nitrate is an anion with a complex bonding structure. Major sources for this ion in drinking water are runoff from fertilizer, septic tank leakage, sewage, and natural deposits. High concentrations of nitrates represent a significant health hazard, especially to infants. The nitrate in the body is converted to nitrite, which then binds to hemoglobin. This binding decreases the ability of hemoglobin to transport oxygen, thus depriving the cells of the O2 needed for proper functioning. Cyanide production is widespread throughout nature. Forest fires will produce significant amounts of cyanide. Many plants contain cyanide, and it is produced by a number of bacteria, algae, and fungi. Cyanide is used industrially in metal finishing, iron and steel mills, and in organic synthesis processes. This material is also an important component for the refining of precious metals. Formation of a complex between cyanide and gold allows extraction of this metal from a mixture. Received: 26 November 2019 Revised: 10 January 2020 Accepted: 19 January 2020 DOI: 10.1111/obr.13005 PEDIATRICS/PHYSIOLOGY Adipokines: A gear shift in puberty Desirée Nieuwenhuis | Natàlia Pujol-Gualdo Amanda J. Kiliaan Department of Anatomy, Radboud university medical center, Donders Institute for Brain, Cognition and Behaviour, Preclinical Imaging Center PRIME, Nijmegen, The Netherlands Correspondence Amanda J. Kiliaan, PhD, Associate Professor, Department of Anatomy, Donders Institute for Brain, Cognition, and Behaviour, Preclinical Imaging Center PRIME, Radboud university medical center, 6500 HB Nijmegen, Geert Grooteplein 21N 6525 EZ Nijmegen, The Netherlands. Email: amanda.kiliaan@radboudumc.nl Funding information Europees Fonds voor Regionale Ontwikkeling (EFRO), Grant/Award Number: BriteN 2016 1 | INTRODUCTION The prevalence of obesity in adolescents and children is increasing in | Ilse A.C. Arnoldussen | Summary In this review, we discuss the role of adipokines in the onset of puberty in children with obesity during adrenarche and gonadarche and provide a clear and detailed overview of the biological processes of two major players, leptin and adiponectin. Adipokines, especially leptin and adiponectin, seem to induce an early onset of puberty in girls and boys with obesity by affecting the hypothalamic-pituitary- gonadal (HPG) axis. Moreover, adipokines and their receptors are expressed in the gonads, suggesting a role in sexual maturation and reproduction. All in all, adipokines may be a clue in understanding mechanisms underlying the onset of puberty in child- hood obesity and puberty onset variability. KEYWORDS adipokines, obesity, puberty 1,2 the age of 5 years were overweight or were with obesity in 2016, and 3 Obesity is defined by an excessive accumulation of white adipose tissue (WAT), and it is often indicated by a body mass index (BMI) 4 above 30. Two main types of adipose tissue were described: WAT and brown adipose tissue (BAT), which differ in morphology and func- 5-7 Ilse A.C. Arnoldussen and Amanda J. Kiliaan contributed equally to this work. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. © 2020 The Authors. Obesity Reviews published by John Wiley & Sons Ltd on behalf of World Obesity Federation Obesity Reviews. 2020;21:e13005. wileyonlinelibrary.com/journal/obr 1 of 10 https://doi.org/10.1111/obr.13005 alarming rates. Specifically, worldwide, 41 million children below this number is expected to increase to 70 million in 2025. obesity is associated with various severe health complications, includ- ing increased risk of diabetes mellitus type 2, hypertension, heart dis- eases, and disturbances in sex hormone levels. 5,6 and mitochondria and plays a role in thermogenesis. Adipocytes in tion. BAT consists of adipocytes containing multiple lipid droplets WAT contain only a few mitochondria and a single lipid droplet. Adipose tissue has several functions including the storage of energy, thermogenesis, and the production and secretion of adipokines Generally, two physiological processes, adrenarche and gonadarche, 11,24 Childhood 5,7,8 a key role in puberty onset. Puberty is known as a period through which the body changes physically, being a physiological process resulting in the maturation of children, i.e. they develop sexual characteristics and obtain reproduc- 9,11 Adipokines are involved in a number of physiological processes including blood pressure, metabo- lism, glucose, and vascular homeostasis and may play amongst others 8-10 (hormones, cytokines, and peptides). tive functions. between obesity and puberty,2,12-23 the biological mechanisms under- lying obesity and puberty onset remain unclear. Hereafter, we review in detail the role of adipokines in the onset of puberty in childhood obesity. Although many studies have shown associations 2 | INITIATION OF PUBERTY PHYSIOLOGICAL PROCESSES IN THE interact to regulate the onset of puberty. During adrenarche, the adrenal cortex secretes steroid hormones (including 2 of 10 NIEUWENHUIS ET AL. androstenedione, dehydroepiandrosterone, dehydroepiandrosterone sulfate (DHEAS), androstenedione, and cortisol), insulin-like growth factor, and growth hormone, which contribute to the pubertal insights on new genetic loci (e.g. melanocortin-4 receptor, mitochon- drial carrier 2, and mitogen-activated protein kinase 13) and on sev- eral pathways that regulate the timing of puberty; however, it partly 34 9,24,25 Both adrenarche and gonadarche are involved in the development growth spurt, body odor, skin oiliness, and skeletal maturation. explains puberty timing variation. Thereby, defining the role of 25 adipokines is of importance in elucidating the variability in puberty as the expression of adipokines is sex-specific and is altered with body composition, adiposity, and during growth spurts. Moreover, adipokines and their receptors are expressed in gonads and several brain regions suggesting involvement in the onset of puberty and sex- ual maturation. Lastly, adipokines interfere in processes regulating timing and duration of puberty, for instance in the HPA and HPG axes which are both key players during adrenarche and gonadarche. Involvement of adipokines in the onset of puberty and specifically in individuals with obesity will be further reviewed in the next 2,24 3 | Puberty onset in girls is assessed using different markers, such as thelarche (breast development), menarche (the start of of pubic hair. pituitary-gonadal (HPG) axis is activated,2,26 and several hormones have been identified to participate in the activation of the HPG axis During gonadarche (Figure 1), the hypothalamic- 2,27 Kisspeptin, neurokinin B, and dynorphin are released by specialized including kisspeptin, neurokinin B, dynorphin, leptin, and ghrelin. 28 key regulator of the pulsatile secretion of gonadotropin releasing neurons, the KNDy neurons in the hypothalamus. Kisspeptin is a 29,30 B stimulates, and dynorphin inhibits the release of kisspeptin, which hormone (GnRH) from the hypothalamus. In addition, neurokinin implies that both coordinate a pulsatile release of kisspeptin. 31 Sub- sections. sequently, the activated HPG axis induces the pituitary gland to secrete luteinising hormone (LH) and follicle stimulating hormone (FSH). As a result, gametogenesis occurs, and the gonads will release sex hormones. Consequently, secondary sex characteristics develop including breast development in girls and an increased testicular vol- 2,26,32 is possibly due to differences in levels of body fat, hypothalamic-pitui- THE ONSET OF PUBERTY IN GIRLS ume in boys. The age at puberty onset varies greatly among individuals, which 19 35 menstruation), and pubic hair development. 33 genome-wide association studies have provided important new tary-adrenal (HPA) axis activity, and genetic background. Recent The average age of However, this age differs between cultures and ethnicities, and since 1980, age at menarche is girls at start of menarche is 12.4 years. 36 significantly decreasing. 36-39 F I G U R E 1 Hormonal regulation in the initiation of puberty in boys and girls. The secretion of kisspeptin, neurokinin B, and dynorphin from KNDy neurons initiate the release of gonadotropin releasing hormone (GnRH) from the hypothalamus. This activates the pituitary gland to produce and secrete luteinising hormone (LH) and follicle stimulating hormone (FSH), which in turn stimulate the gonads to produce estrogen and testosterone in girls and boys, respectively 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License NIEUWENHUIS ET AL. 3 of 10 T A B L E 1 Summary of included studies Authors Year Country Study Design Primary Outcome Sex Sample Size (n) Age (y) Data Collection Lian et al21 2019 China Cross-sectional Puberty starts earlier in Chinese Han girls with obesity compared with Chinese Han girls with normal weight. Girls 2996 9-19 2012 and 2013 Biro et al12 Lazzeri et al20 2018 USA 2018 Italy Longitudinal Cross-sectional Body mass index had a greater effect on age at menarche than did race and ethnicity. Girls 946 6-16 2004-2014 Li et al23 2018 China Longitudinal For both, boys and girls, a higher BMI (ie, overweight and obese) is associated with earlier onset of puberty Girls Girls Boys Girls 542 Deng et al22 Flom et al15 2017 China Cross-sectional Increased BMI is associated with early timing spermarche and menarche. Boys Girls Girls 1278258 9-15 2005-2012 He et al24 Holmgren et al17 2017 China 2017 Sweden Cross-sectional Longitudinal Onset of puberty is not related to obesity in boys. Boys Boys Girls Girls 782 7-17 972 929 5839 Kelly et al19 2017 UK 2016 Brazil 2016 USA Longitudinal prospective cohort Higher BMI in girls is associated with the onset of menstruation at an earlier age. 11 10-18 11-17 Barcellos Gemelli et al25 Cross-sectional Longitudinal Excess weight is associated with early age of menarche. Girls 727 2014 2003-2009 Glass et al16 Lee et al26 In girls, but not in boys, greater adiposity is associated with the earlier onset of puberty. Boys Girls 135 Cabrera et al27 Leonibus et al14 2014 USA 2013 Italy Cross-sectional Longitudinal Thelarche occurred earlier than recently reported, while age of menarche remained unchanged. Girls 610 3-17.9 2007 2005-2012 Currie et al13 2012 Europe, USA, Canada Cross-sectional Overweight/obesity during childhood predicts the early onset of puberty in girls. Girls 20410 11, 13, 15 2005-2006 2017 USA Prospective birth cohort Overweight/obese status at the age of 7 ye was associated with increased risk of early menarche 788 From birth to menarche occurred Pregnancies 1959-1966 2016 USA Cross-sectional Boys with overweight enter puberty earlier compared with boys with normal weight or obesity, while puberty starts later in boys with obesity compared with boys with normal weight and overweight. Boys 3872 6-16 2005-2010 Overweight during childhood shows a relation with the early onset of puberty in girls. 6535 4259 695 11 15 5.8-12.2 2009/2010 2013/2014 2014-2017 Higher BMI during childhood is associated with early puberty. 2008 and 2009 2000-2002 Obesity during childhood is related to the earlier onset of puberty. Boys Girls 84 123 71 (Continues) 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 4 of 10 NIEUWENHUIS ET AL. 3.1 | Fat storage For the initiation of puberty, the timing of stimulation and/or inhibi- tion of different hormones is important, and additionally, a certain amount and distribution of body fat is needed in order to start menar- che, which emphasizes the importance of body fat. From an evolution- ary point of view, body fat increases in mammalian females during puberty onset, and it highlights the need to guarantee a healthy preg- 40 women with anorexia nervosa. particularly body fat localized predominantly on the gluteofemoral fat depots, is profoundly associated with start of menarche, more than nancy, offspring, and maternal survival. fat, sex-hormones, and neuroendocrine alterations can evolve in men- strual dysfunction, for instance, in women with severe obesity or in 41-43 44-46 to gluteofemoral fat depots suggesting that leptin may convey infor- amount of total body fat. mation on body fat distribution to the hypothalamus during puberty. An improper level of body Importantly, body fat distribution, Blood leptin levels are strongly related 45 3.2 | HPG axis The HPG axis is activated by the release of kisspeptin resulting in the release of GnRH from the hypothalamus, and LH and FSH from the pituitary gland. In girls, FSH is involved in the development of the folli- cles in the ovaries, and it promotes the secretion of estrogen. LH stim- ulates the production of androgen hormones and induces ovulation 48 9,47 the release of kisspeptin and neurokinin B, and kisspeptin thereby (Figure 1). The secretion of estrogen has an inhibitory effect on inhibits the GnRH release from the hypothalamus. pattern of GnRH is important for the regulation of the menstrual cycle. This roughly 28-day-cycle comprises several phases, including the follicular phase and luteal phase. During the follicular phase, increasing levels of FSH stimulate the maturation of follicles and the production of estrogen from the ovaries. This in turn inhibits the release of FSH from the pituitary gland. A high level of estrogen will induce the production of LH by the pituitary gland, resulting in ovula- tion. The matured follicle secretes progesterone thereby inhibiting the release of GnRH. When the corpus luteum is demolished, there is less 48 3.3 | Adipokines According to results from studies reported in Table 1, girls with obe- sity enter puberty earlier compared with girls with normal higher leptin concentrations inhibit the intake of food and increases inhibition of GnRH. As a consequence, the cycle will start again. whole process, starting from the activated HPG axis, results in the development of the secondary sex characteristics in girls including 9,47 thelarche and menarche. 13,14,16-23,49-51 weight. these girls might be found in the secretion of adipokines. For instance, leptin is positively associated with the amount of body fat. Generally, energy expenditure. 9,52-54 An explanation for the early onset of puberty in The expression This TABLE 1 (Continued) Authors Year Country Study Design Primary Outcome Sample Sex Size (n) Age (y) Data Collection Herman-Giddens et al28 2012 USA Cross-sectional Observed mean ages of beginning genital and pubic hair growth and early testicular volumes were earlier than in past studies, depending on the characteristic and race/ethnicity. Boys 4131 6-16 2005-2010 Sorensen et al29 Aksglaede et al30 2010 2009 Denmark Denmark Cross-sectional/longitudinal Longitudinal Puberty onset at earlier ages was associated with an increased BMI in boys. Boys 1528 5.8-19.9 1991-1993/2006-2008 1930-1969 Juul et al31 Ribeiro et al32 2007 2006 Denmark Portugal Retrospective cohort Cross-sectional Higher BMI is associated with early voice break. 11-15 10-15 1990-1999 Kaplowitz et al18 Abbreviation: BMI, body mass USA Cross-sectional The early onset of puberty in Caucasian girls is likely related to an increased BMI. 5-12 1992-1993 2001 index. The higher BMI in boys and girls at 7 y of age, the earlier they enter puberty. Boys 21 612 Girls 135 223 Boys 463 Boys 382 Girls 437 Girls 10 750 Early sexual maturation in boys and girls is associated with overweight. 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License NIEUWENHUIS ET AL. 5 of 10 Leptin may possibly play a role in adrenarche as its plasma level increases with higher levels of body fat and as it can modulate both girls. 33 ing adrenarche. In coherence, in children with obesity, the androgen These findings suggested that lower reproductive status was associated with higher total adiponectin concentrations and that a higher reproductive status was related to higher HMW adiponectin the HPA and HPG axes. These axes are functionally integrated dur- DHEAS was positively associated with leptin levels. Nevertheless, concentrations in girls. In addition, individuals with obesity often another study showed that enhanced adrenal androgen secretion in girls with premature adrenarche was not explained by leptin or BMI 55 ated with androgen levels in girls ; however, it was not related to levels. and IL-6. TNF-α alters, and IL-6 inhibits the expression of 56 8 In addition, the adipokine adiponectin was negatively associ- 57 differences of adiponectin seem to develop during the progression of 56 adiponectin (Figure 2). Thereby, a low level of total adiponectin and/or high levels of inflammatory cytokines in individuals with obe- sity can promote the onset of puberty. Many more adipokines are secreted by WAT including omentin, 52,65-67 9,36,62,68 adrenarche in girls with Prader-Willi syndrome. Interestingly, sex puberty. adrenarche; however, both are not required factors. Thus, leptin and adiponectin might be able to influence In gonadarche, leptin can stimulate the secretion of kisspeptin, and subsequently activation of the HPG axis, which eventually increases the expression of estrogen and androstenedione in the ova- 58 2,60 65-67 The expression of these ries (Figure 2). Ob gene in WAT, resulting in the synthesis and secretion of leptin. Thus, high levels of leptin promote onset of puberty in girls via secre- tion of kisspeptin, and estrogen stimulates leptin secretion addition- ally. Moreover, adiponectin can affect the HPG axis due to the expression of adiponectin receptors in the hypothalamus, pituitary In return, estrogen stimulates the expression of the 59 gland, and gonads. onset as it inhibits the secretion of kisspeptin and GnRH in the hypo- thalamus and the release of GH and LH in the pituitary gland, and 2,60-62 52,60 63 girls with central precocious puberty (CPP). Moreover, total adiponectin had negative correlations with progression of puberty in girls (defined by Tanner stages), whereas HMW adiponectin had FIGURE 2 Adipokinesaffectingthe initiation of puberty in girls. Leptin stimulates the release of kisspeptin in KNDy neurons, which activates the hypothalamus to produce gonadotropin releasing hormone (GnRH). In response to the release of GnRH, the pituitary gland secretes follicle stimulating hormone (FSH) and luteinising hormone (LH), which stimulates the ovaries to release estrogen resulting in the formation of secondary sex characteristics in girls. Estrogen stimulates the production of leptin. Adiponectin inhibits GnRH release resulting in reduced levels of GnRH and thereby a delayed onset of puberty. TNF- α and IL-6 inhibit the production of adiponectin and therefore stimulate the onset of puberty In detail, adiponectin is a regulator of puberty thereby inhibiting the onset of puberty (Figure 2). with obesity often have low levels of adiponectin. et al. showed that total adiponectin was significantly lower, whereas high molecular weight (HMW) adiponectin was significantly higher in ment. 55 63 develop a chronic low-grade inflammatory state, which can be indi- cated by a high level of circulating inflammatory cytokines like TNF-α 64 Individuals Sitticharoon positive associations with LH levels and the progression of puberty in 63 visfatin, resistin, and chemerin. and visfatin are expressed in the ovaries. adipokines in the ovaries suggests a role within the reproductive sys- tem; however, the exact biological processes have to be examined. Thus, specifically leptin, adiponectin, and inflammatory cytokines pro- duced by WAT could be permissive key players during an early onset of puberty in girls with obesity. As an exception, HMW adiponectin seems to have a stimulatory effect on peripheral repro- ductive function as HMW is not able to cross the blood brain 63 barrier. 4 | Markers that are used to assess puberty onset in boys are THE ONSET OF PUBERTY IN BOYS spermarche, voice break, testicular volume, and pubic hair develop- 35 spermarche develop in the early stages of puberty onset, voice In women, omentin, chemerin, While pubic hair development, larger testicular volume, and 69 testicular volume increases, which occurs at an average age of break usually appears in later stages of puberty. Generally, first 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 6 of 10 NIEUWENHUIS ET AL. 11.9 years, followed by the development of pubic hair at 12.2 years of average, and lastly, boys experience spermarche around an aver- 55 related with leptin levels. Thereby, leptin plausibly has a minor impact in adrenarche in boys. Since leptin receptors are found in the hypothalamus, pituitary gland, and testes, they might be involved in the onset of puberty by affecting the HPG axis during gonadarche. Leptin stimulates the release of kisspeptin and GnRH, and as a consequence, it accelerates the onset of puberty (Table 1, Figure 3). In contrast, adiponectin inhibits the secretion of GnRH, GH, LH, and FSH therewith delaying the onset of puberty. However, adiponectin levels are generally lower in men compared with women and even lower in men with obe- age age of 13.4 years. 70 4.1 | Fat storage Many aspects of the reproductive physiology are energetically demanding,71 and therefore, an adequate energy level is necessary. In boys, a dynamic change in body composition occurs around the age of 10 to 13 years, in which they gain approximately 40% of sity. culating inflammatory cytokines. levels can stimulate the HPG axis and therewith an early onset of puberty in boys. Nevertheless, leptin can inhibit the production of tes- 72 mostly consisting of lean mass, which causes exhaustion of most of fat. Subsequently, a growth spurt follows in which they gain tissue 72 in boys, an adequate amount of body fat is important in the onset of their body fat. These alterations in amount of body fat indicate that 4.2 | Puberty in boys is initiated by the release of kisspeptin. As mentioned before, this activates the HPG axis, resulting in the release of GnRH from the hypothalamus, and consequently the release of LH and FSH 9,74 puberty. tosterone from the testes, to estrogen (Figure 3). of the development of secondary sex characteristics in boys. Additionally, leptin can affect fertility in men as it can modulate the nutritional support of spermatogenesis, and moreover, dysfunction of spermatogenesis is associated with an increased leptin level and 73 58 2,60-62 HPG axis from the pituitary gland (Figure 1). and LH stimulates the secretion of testosterone from the testes, which inhibits the release of kisspeptin from the KNDy neurons and 9,48 in men, the release of kisspeptin is more consistent, causing a con- 29,48 subsequently GnRH from the hypothalamus. receptors expressed on KNDy neurons. In humans, KNDy neurons Contrarily to women, LH-induced testosterone levels lead to the stant release of LH. development of secondary sex characteristics in boys. differences between sexes in kisspeptin release are related to a sex- specific and sex steroid-dependent kisspeptin system as estrogen and progesterone modulate kisspeptin activity through the sex-steroid 48 in the infundibular nucleus are involved in negative and positive sex- 48 tal exposure to sex steroids and result in sex-specific differences in steroid feedbacks. kisspeptin release. These sexual dimorphisms are induced by perina- 75,76 4.3 | Adipokines The association between obesity and puberty onset in boys is rather controversial compared with findings in girls. Most studies reported an early onset of puberty in boys associated with increased ate adipose tissue from actual breast tissue. stages are more difficult to assess than female stages as boys lack a more determined marker such as menarche. Thirdly, puberty onset can be indicated by the activation of the HPG axis, and the presence of these secondary sex characteristics is the result of hormonal 2 14,17,22,23,50,51,77,78 BMI, 20,49 all while others reported no associations at Current markers used 79 16,80 or a delayed onset of puberty (Table 1). The presence of excessive adipose tissue can be involved in puberty onset in boys as the secretion of adipokines can modulate both adrenarche and gonadarche. Leptin can affect adrenarche by modulating both the HPG and HPA axes,33 and moreover, androgen levels were positively 55 nal androgen secretion in boys with premature adrenarche was not associated with plasma leptin levels. Nevertheless, enhanced adre- 9 In more detail, 61,62 adiponectin, and individuals with obesity often have high levels of cir- Moreover, inflammatory cytokines, TNF-α, and IL-6, inhibit expression of the leptin receptor in the testis. FSH induces spermatogenesis, too. function and role still have to be examined. 64 High leptin and low adiponectin and fat tissue can convert testosterone Both processes might result in the delay 29,61,79 81,82 In men, other adipokines like chemerin are found in the gonads 65 Thus, particularly high leptin and low adiponectin levels stimulate the HPG axis and thereby accelerate the onset of puberty in boys. Additionally, leptin can dysregulate the development of secondary sex characteristics and spermatogenesis by affecting testosterone levels and nutritional sup- port of spermatogenesis. 5 | LIMITATIONS AND FUTURE RESEARCH DIRECTIONS Even though multiple epidemiological studies have shown the link between puberty onset and obesity, there are some important limita- tions. Firstly, determining both the onset and stage of puberty is rather difficult. For instance, assessing the stage of breast develop- ment in girls with obesity is complicated as clinicians should differenti- 2 changes in response to the activated HPG axis. to determine the onset of puberty refer to secondary sex characteris- tics, such as testicular volume in boys and breast development in girls. A more accurate measurement of puberty onset would be to combine secondary sex characteristics with plasma or serum hormone level measurements such as LH, FSH, adipokines, e.g. leptin. Thereby, differences in puberty measurements could explain variations in the age of puberty onset between boys and girls within different Thereby, resistin is expressed in the testes of rats, but its exact 83 Secondly, male pubertal 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License NIEUWENHUIS ET AL. 7 of 10 FIGURE 3 Adipokines affecting the initiation of puberty in boys. Leptin activates kisspeptin secretion in KNDy neurons, this activates the production of gonadotropin releasing hormone (GnRH) from the hypothalamus. GnRH stimulates the pituitary gland to secrete follicle stimulating hormone (FSH) and luteinising hormone (LH), activating the production of testosterone from the testes allowing the development of secondary sex characteristics. Leptin also inhibits the production of testosterone, which may cause a delayed onset of puberty. Adiponectin inhibits GnRH release. Low levels of adiponectin, as a result of TNF-α and IL-6 expression, lead to a reduced inhibition of GnRH. In response to GnRH release, the pituitary gland will secrete FSH and LH, and the testes will produce testosterone resulting in the development of secondary sex characteristics in boys countries, and In addition, the inclusion of a of puberty. ferent time points is complicated, as subjects examined several decades ago presented pronounced differences concerning lifestyle patterns such as nutrition and exercise habits. Lastly, obesity or over- weight is often determined by BMI, a classification based on weight and height measurements. Additionally, it is important that all studies studies or across continents, ethnicities proper age range (8-16 years) is important when assessing the onset (Figure 4). 12-15,17,20-23,49,77-79,84,85 30,47 Furthermore, comparison between studies from dif- 86 Specifically in children, BMI is often dependent on age and growth use the same anthropometric standards and sex-specific cut-offs. 13,14,16-23,49-51,77-80 fat and would represent a more accurate measurement in its regard. Based on this review, several suggestions can be made for further research. Firstly, the roles of adipokines like resistin, chemerin, visfatin, and omentin in puberty onset, fertility, and sexual maturation should be examined in detail. Secondly, future research examining the onset of puberty should combine indicators of puberty onset (e.g. breast development or testicular volume) with plasma or serum hor- mone measurements such as LH, FSH, sex-steroids, adipokines (e.g. spurts. ment in case of growth spurts. distribution of body fat should be taken into account in determining puberty and obesity in children. For instance, the body adiposity index (BAI), which was introduced in 2011 by Bergman et al.,87 uses hip cir- cumference and height in order to estimate the percentage of body 87 Thereby, BMI is a less accurate measure- F I G U R E 4 87,88 Therefore, both percentage and Average age of puberty onset in Europe, China, and the United States according to several studies from Table 1. Age of puberty onset ranges from 8.47 to 13.33 years in girls and from 8.63 leptin), and body fat distribution (e.g. BAI,87 waist-hip ratio's and/or dual-energy X-ray absorptiometry (DXA)2). Additionally, defining con- sistent and general measurements of puberty in both boys and girls, combined with a proper age range (8-16 years), would facilitate the comparisons between different studies and their results. 12-15, 17, 20-23, 25-29, 31 to 13.7 years in boys. included if average age of markers used to assess puberty was not reported. Pink: girls. Blue: boys Studies (Table 1) were not 39, 56 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are governed by the applicable Creative Commons License 8 of 10 NIEUWENHUIS ET AL. 6 | CONCLUSION In conclusion, epidemiological data regarding obesity and puberty onset in girls show similar outcomes as adiposity results in the early onset of puberty in girls. The majority of the studies examining boys with obesity indicate an early onset of puberty, while not all reported an earlier onset of puberty. In detail, high leptin, TNF-α, and IL-6 levels combined with low adiponectin levels stimulate the activation of the HPG axis in girls and boys with obesity, and 5, 45, 50, 51 REFERENCES 1. Kumar S, Kelly AS. Review of childhood obesity: from epidemiology, etiology, and comorbidities to clinical assessment and treatment. May- o Clin Proc. 2017;92(2):251-265. 2. Reinehr T, Roth CL. Is there a causal relationship between obesity and puberty? The Lancet Child & adolescent health. 2019;3(1):44-54. 3. WorldHealthOrganization. Facts and figures on childhood obesity. 2017. 4. Guglielmi V, Sbraccia P. Obesity phenotypes: depot-differences in adipose tissue and their clinical implications. Eat Weight Disord. 2018; 23(1):3-14. 5. Gomez-Hernandez A, Beneit N, Diaz-Castroverde S. Escribano O. 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FUNDING INFORMATION This research was funded by Europees Fonds voor Regionale Ontwikkeling (EFRO), project BriteN 2016. ORCID Ilse A.C. Arnoldussen Amanda J. Kiliaan https://orcid.org/0000-0002-7395-5284 https://orcid.org/0000-0002-2158-6210 13, 14, 16-26, 29-32 Furthermore, several receptors Nevertheless, We conclude Search strategy We searched PubMed for articles published before Novem- ber 15th, 2019 using relevant keywords, including ‘onset of puberty and adiposity/obesity’, ‘onset of puberty’, ‘children with obesity’, ‘adipose tissue’, ‘childhood obesity’, ‘adiposity’, ‘obesity’, ‘adipokine(s)’, ‘HPG axis’, ‘adipokines ovary/ova- ries’, or ‘adipokines testes’, either alone or in combination. 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Secondary sexual characteristics in boys: data from the Pediatric Research in Office Settings Network. Pediatrics. 2012;130(5):e1058-e1068. 86. WHO. Physical status: the use and interpretation of anthropometry. Report of a WHO Expert Committee. World Health Organ Tech Rep Ser. 1995;854:1-452. 87. Akin I, Tolg R, Hochadel M, et al. No evidence of “obesity paradox” after treatment with drug-eluting stents in a routine clinical practice: results from the prospective multicenter German DES.DE (German Drug-Eluting Stent) Registry. JACC Cardiovasc Interv. 2012;5(2): 162-169. 88. Marcovecchio ML, Chiarelli F. Obesity and growth during childhood and puberty. World Rev Nutr Diet. 2013;106:135-141. How to cite this article: Nieuwenhuis D, Pujol-Gualdo N, Arnoldussen IAC, Kiliaan AJ. Adipokines: A gear shift in puberty. Obesity Reviews. 2020;21:e13005. https://doi.org/ 10.1111/obr.13005 1467789x, 2020, 6, Downloaded from https://onlinelibrary.wiley.com/doi/10.1111/obr.13005, Wiley Online Library on [10/03/2024]. See the Terms and Conditions (https://onlinelibrary.wiley.com/terms-and-conditions) on Wiley Online Library for rules of use; OA articles are goverPlant cells have three kinds of structures that are not found in animal cells and that are extremely important to plant survival: plastids, central vacuoles, and cell walls. PLANT CELLS Most of the organelles and other parts of the cell just described are common to all eukaryotic cells. However, plant cells have three additional kinds of structures that are extremely important to plant function: cell walls, large central vacuoles, and plastids. To understand why plant cells have structures not found in ani- mal cells, consider how a plant’s lifestyle differs from an animal’s. Plants make their own carbon-containing molecules directly from carbon taken in from the environment. Plant cells take carbon diox- ide gas from the air, and in a process called photosynthesis, they convert carbon dioxide and water into sugars. The organelles and structures in plant cells are shown in Figure 4-21. SECTION 4 OBJECTIVES ● List three structures that are present in plant cells but not in animal cells. ● Compare the plasma membrane, the primary cell wall, and the secondary cell wall. ● Explain the role of the central vacuole. ● Describe the roles of plastids in the life of a plant. ● Identify features that distinguish prokaryotes, eukaryotes, plant cells, and animal cells. VOCABULARY cell wall central vacuole plastid chloroplast thylakoid chlorophyll Chloroplast Golgi apparatus Mitochondrion Cell membrane Nucleolus Nucleus Cytoskeleton Rough endoplasmic reticulum Pore Smooth endoplasmic reticulum Central vacuole Ribosome Cell wall In addition to containing almost all of the types of organelles that animal cells contain, plant cells contain three unique features. Those features are the cell wall, the central vacuole, and plastids, such as chloroplasts. FIGURE 4-21 Copyright © by Holt, Rinehart and Winston. All rights reserved. 88 CHAPTER 4 CELL WALL The cell wall is a rigid layer that lies outside the cell’s plasma membrane. Plant cell walls contain a carbohydrate called cellulose. Cellulose is embedded in a matrix of proteins and other carbohy- drates that form a stiff box around each cell. Pores in the cell wall allow water, ions, and some molecules to enter and exit the cell. Primary and Secondary Cell Walls The main component of the cell wall, cellulose, is made directly on the surface of the plasma membrane by enzymes that travel along the membrane. These enzymes are guided by microtubules inside the plasma membrane. Growth of the primary cell wall occurs in one direction, based on the orientation of the microtubules. Other components of the cell wall are made in the ER. These materials move in vesicles to the Golgi and then to the cell surface. Some plants also produce a secondary cell wall. When the cell stops growing, it secretes the secondary cell wall between the plasma membrane and the primary cell wall. The secondary cell wall is very strong but can no longer expand. The wood in desks and tabletops is made of billions of secondary cell walls. The cells inside the walls have died and disintegrated. CENTRAL VACUOLE Plant cells may contain a reservoir that stores large amounts of water. The central vacuole is a large, fluid-filled organelle that stores not only water but also enzymes, metabolic wastes, and other materials. The central vacuole, shown in Figure 4-22, forms as other smaller vacuoles fuse together. Central vacuoles can make up 90 percent of the plant cell’s volume and can push all of the other organelles into a thin layer against the plasma membrane. When water is plentiful, it fills a plant’s vacuoles. The cells expand and the plant stands upright. In a dry period, the vacuoles lose water, the cells shrink, and the plant wilts. Other Vacuoles Some vacuoles store toxic materials. The vacuoles of acacia trees, for example, store poisons that provide a defense against plant-eating ani- mals. Tobacco plant cells store the toxin nicotine in a storage vacuole. Other vacuoles store plant pigments, such as the colorful pigments found in rose petals. The central vacuole occupies up to 90 percent of the volume of some plant cells. The central vacuole stores water and helps keep plant tissue firm. FIGURE 4-22 Central vacuole Nucleus Chloroplast Copyright © by Holt, Rinehart and Winston. All rights reserved. CELL STRUCTURE AND FUNCTION 89 PLASTIDS Plastids are another unique feature of plant cells. Plastids are organelles that, like mitochondria, are surrounded by a double mem- brane and contain their own DNA. There are several types of plastids, including chloroplasts, chromoplasts, and leucoplasts. Chloroplasts Chloroplasts use light energy to make carbohydrates from carbon dioxide and water. As Figure 4-23 shows, each chloroplast contains a system of flattened, membranous sacs called thylakoids. Thylakoids contain the green pigment chlorophyll, the main mole- cule that absorbs light and captures light energy for the cell. Chloroplasts can be found not only in plant cells but also in a wide variety of eukaryotic algae, such as seaweed. Chloroplast DNA is very similar to the DNA of certain photosyn- thetic bacteria. Plant cell chloroplasts can arise only by the divi- sion of preexisting chloroplasts. These facts may suggest that chloroplasts are descendants of ancient prokaryotic cells. Like mitochondria, chloroplasts are also thought to be the descendants of ancient prokaryotic cells that were incorporated into plant cells through a process called endosymbiosis. Chromoplasts Chromoplasts are plastids that contain colorful pigments and that may or may not take part in photosynthesis. Carrot root cells, for example, contain chromoplasts filled with the orange pigment carotene. Chromoplasts in flower petal cells contain red, purple, yellow, or white pigments. Other Plastids Several other types of plastids share the general features of chloro- plasts but differ in content. For example, amyloplasts store starch. Chloroplasts, chromoplasts, and amyloplasts arise from a common precursor, called a proplastid. Thylakoid Inner membrane Outer membrane chloroplast from the Greek chloros, meaning “pale green,” and plastos, meaning “formed” Word Roots and Origins A chloroplast captures energy from sunlight and uses that energy to convert carbon dioxide and water into sugar and other carbohydrates. FIGURE 4-23 Copyright © by Holt, Rinehart and Winston. All rights reserved. 90 CHAPTER 4 COMPARING CELLS All cells share common features, such as a cell membrane, cyto- plasm, ribosomes, and genetic material. But there is a high level of diversity among cells, as shown in Figure 4-24. There are signifi- cant differences between prokaryotes and eukaryotes. In addition, plant cells have features that are not found in animal cells. Prokaryotes Versus Eukaryotes Prokaryotes differ from eukaryotes in that prokaryotes lack a nucleus and membrane-bound organelles. Prokaryotes have a region, called a nucleoid, in which their genetic material is concen- trated. However, prokaryotes lack an internal membrane system. Plant Cells Versus Animal Cells Three unique features distinguish plant cells from animal cells. One is the production of a cell wall by plant cells. Plant cells contain a large central vacuole. Third, plant cells contain a variety of plastids, which are not found in animal cells. Cell walls, central vacuoles, and plastids are unique features that are important to plant function. 1. Identify three unique features of plant cells. 2. List the differences between the plasma mem- brane, the primary cell wall, and the secondary cell wall. 3. Identify three functions of plastids. 4. Name three things that may be stored in vacuoles. 5. Describe the features that distinguish prokary- otes from eukaryotes and plant cells from animal cells. CRITICAL THINKINGSure! Here's a solid list of **AP English Literature vocabulary**—terms that often come up in class, essays, and the AP exam. I'll break it down into categories to make it easier to study. --- ### 📚 **Literary Devices & Techniques** 1. **Alliteration** – Repetition of initial consonant sounds 2. **Allusion** – A reference to another text, event, or figure 3. **Anaphora** – Repetition of a word or phrase at the beginning of successive clauses 4. **Antithesis** – Contrast of ideas in a balanced or parallel construction 5. **Apostrophe** – Addressing someone absent, dead, or nonhuman as if present and able to respond 6. **Assonance** – Repetition of vowel sounds within nearby words 7. **Asyndeton** – Omission of conjunctions between parts of a sentence 8. **Consonance** – Repetition of consonant sounds, often at the end of words 9. **Diction** – Word choice (formal, informal, colloquial, etc.) 10. **Enjambment** – Continuation of a sentence without pause beyond the end of a line in poetry --- ### 🧠 **Figurative Language** 1. **Hyperbole** – Extreme exaggeration 2. **Imagery** – Descriptive language that appeals to the senses 3. **Irony** - *Verbal*: Saying the opposite of what’s meant - *Situational*: When the outcome is the opposite of what's expected - *Dramatic*: Audience knows something characters don’t 4. **Metaphor** – A direct comparison without using "like" or "as" 5. **Metonymy** – Substituting the name of one thing with something closely related (e.g. "The crown" for royalty) 6. **Synecdoche** – A part representing the whole (e.g. "All hands on deck") 7. **Personification** – Giving human traits to nonhuman things 8. **Simile** – A comparison using "like" or "as" 9. **Symbol** – An object, character, or color that represents something beyond itself --- ### ✍️ **Poetic & Rhetorical Terms** 1. **Caesura** – A pause in a line of poetry, often marked by punctuation 2. **Couplet** – Two lines of poetry that usually rhyme 3. **Iambic Pentameter** – A line with five iambs (unstressed-stressed syllables) 4. **Blank Verse** – Unrhymed iambic pentameter 5. **Free Verse** – Poetry with no fixed meter or rhyme 6. **Elegy** – A mournful poem, often for the dead 7. **Ode** – A lyric poem expressing emotion, often in honor of something 8. **Sonnet** – A 14-line poem with a specific rhyme scheme (Shakespearean or Petrarchan) --- ### 📖 **Narrative & Structure Terms** 1. **Tone** – The author's attitude toward the subject 2. **Mood** – The feeling or atmosphere the reader experiences 3. **Theme** – The central idea or message in a work 4. **Motif** – A recurring element that has symbolic significance 5. **Foil** – A character who contrasts with another character to highlight traits 6. **Foreshadowing** – Clues or hints about what will happen later 7. **Juxtaposition** – Placing two elements side by side to present a contrast 8. **Point of View** – Perspective from which the story is told (1st, 2nd, 3rd person) 9. **Stream of Consciousness** – Narrative style that mimics thoughts and feelings 10. **Frame Narrative** – A story within a story --- Want me to make flashcards, a quiz, or a PDF study guide with these? Or need help using them in a literary analysis essay?Giulio cesare nacque nel centro avanti cristo e morì nel senso che fu ucciso nel 44 avanti cristo in una vita relativamente breve 56 anni che si colloca nel momento di passaggio drammatico da un sistema politico ad un altro dall'ordinamento tradizionale della città stato di roma ad un tipo di potere personale fortemente personale quindi la vita è di cesare proprio nel cuore di questo periodo quando affiora alla adolescenza all'uso della ragione egli è già un personaggio un personaggio malvisto perché si è legato per ragioni familiari tradizionali di schieramento anche politico alla famiglia di cinna fedele alleato e seguace di caio mario quindi sotto la dittatura di sila sicuramente un nemico ed e noi abbiamo su di lui varie biografie ma un elemento che ricorre in queste biografie e che silla uomo indubbiamente di grande capacità politica di grande intuito e di grande ferocia era convinto che andasse eliminato questo giovanotto perché pericoloso perché è talmente in gamba lo si vedeva già da giovanissimo da costituire un pericolo per l'ordinamento che sigla aveva instaurato secondo la tradizione roma viene fondata nel 753 avanti cristo da romolo che diventa anche il primo re della città per oltre due secoli roma è retta da una monarchia fino a quando tarquinio il superbo il settimo e ultimo re viene cacciato e viene instaurata la repubblica l'ordinamento repubblicano rimarrà in vigore per quasi cinque secoli durante i quali i romani porranno le basi del loro imponente impero la prima fase di questa espansione è dedicata alla conquista della penisola italiana tra il iii e il ii secolo avanti cristo roma allarga il proprio dominio su tutto il bacino del mediterraneo giungendo fino all'asia minore a farne le spese sono i cartaginesi di annibale ambientati nel corso delle tre guerre puniche e i macedoni la cui sconfitta segna la fine della potenza greca in questo lungo periodo lo stato romano si trova ad affrontare numerosi conflitti interni le lotte tra patrizi e plebei le rivolte popolari i durissimi scontri tra fazioni avverse tra il 133 121 i tribuni della plebe tiberio e gaio gracco propongono alcune riforme agrarie che mirano a una ridistribuzione delle terre in favore dei piccoli proprietari osteggiate da gli strati sociali più ricchi le riforme dei due fratelli vengono abolite ma segnano l'inizio di un lunghissimo periodo di scontri interni noto col nome di guerre civili che dura esattamente un secolo quando nasce cesare nel 100 avanti cristo la repubblica romana sta vivendo perciò una profonda crisi che ne mina le fondamenta al principio del primo secolo esplode un'insurrezione nelle province italiane i cui abitanti chiedono maggiori diritti è la cosiddetta guerra sociale repressa duramente dall'esercito romano capeggiato dal generale lucio cornelio silla silla sarà protagonista anche di una guerra civile che vede contrapposta la passione degli optimates gli aristocratici difensori dello status quo e i popularis i nobili favorevoli al cambiamento istituzionale il sanguinoso scontro vedrà la vittoria degli optimates di silla che nell 82 viene nominato dittatore con il compito di tentare una difficile pacificazione politica sia stato il trionfatore di una guerra civile ferocissima contro i mariani seguaci di caio mario ci dice in maniera sommaria e grossomodo ha un senso che capeggia uno schieramento conservatore sorretto da gli optimates e contrasta uno schieramento popularis di cui mario è l'uomo simbolo alla fine silla trionfare in questo conflitto i suoi avversari vengono fisicamente liquidati e la sua dittatura la dittatura esiste nell'ordinamento romano ma a termine cioè non può essere una carica vitalizia la sua dittatura stabilisce un un riordino un nuovo ordine della repubblica romana quindi si crea una situazione paradossale il potere personale fortissimo riordino dell'ordinamento tradizionale è fatta quest'opera di riordino costituzionale abbandono del potere si la compie un gesto indubbiamente straordinario a suo modo le ragioni per cui l'ha compiuto sono molteplici lascia la dittatura abbandona questo potere lo dismette per assistere da vecchio ormai morirà nell'anno 78 quindi poco dopo l'abbandono realizza tour a 78 avanti cristo per assistere al funzionamento del riordino che egli stesso aveva creato illusione ottica perché nell'anno 70 quindi appena pochi anni dopo cesare nel frattempo ha raggiunto i 30 anni i due consoli dell'anno 70 che sono niente meno che marco licinio crasso l'uomo più ricco di roma e che neo pompeo figlio di pompeo strabone pur rappresentando sostanzialmente forze sociali e politiche che consiglia erano state strettamente alleate abrogano l'ordinamento silano quindi con l'anno 70 si determinano una alleanza saldissima parrebbe dei due più potenti personaggi che sono sulla scena della politica romana un'alleanza personale politica e istituzionale attraverso la copertura del consolato che sembra stabilire un ritorno all ordinamento repubblicano tradizionale ivi compresa la restituzione ai tribuni della plebe delle loro importanti prerogative al tempo di giulio cesare la repubblica romana a oltre 400 anni di vita un lungo periodo durante il quale ha sviluppato un efficace organizzazione amministrativa l'esercizio del potere si fonda sulla suddivisione dei compiti tra il senato le assemblee popolari e i funzionari pubblici i cosiddetti magistrati il senato è l'istituzione che prende le decisioni più importanti in politica interna ed estera le assemblee popolari rappresentano l'elemento democratico della repubblica vi partecipano tutti i cittadini romani liberi escluse le donne e hanno come compito principale l'elezione dei magistrati i magistrati costituiscono il cardine della vita quotidiana ad essi infatti è affidata la gestione concreta dello stato ci sono innanzitutto i questori che si occupano delle finanze dell'erario gli edili che sovrintendono ai lavori pubblici al controllo dei prezzi e all'organizzazione dei giochi e i pretori a cui è demandata l'amministrazione della giustizia una figura di grande responsabilità è quella del tribuno della plebe istituita in seguito agli aspri conflitti sociali dei primi anni della repubblica i tribuni della plebe tutelano i diritti delle classi popolari e sono considerati sacri e inviolabili tra i magistrati la massima carica è quella dei consoli coloro che detengono il governo della città i consoli sono due esercitano il potere esecutivo e militare convocano e presiedono al senato comandano l'esercito in caso di guerra tutti questi incarichi hanno la durata di un anno e vengono ricoperti collegialmente una maniera questa per evitare le possibili tendenze dispotiche o monarchiche solo in caso di grave pericolo viene eletto il dittatore un governatore straordinario che assume in sé tutti i poteri fino alla conclusione dello stato di emergenza questa carica comunque può durare al massimo sei mesi vi è infine una carica religiosa che riveste un ruolo determinante nella vita di roma è il pontefice massimo che vigila sull'ortodossia del culto e presenzia alle manifestazioni pubbliche più importanti una figura influente anche dal punto di vista politico come comprende acutamente giulio cesare che per aiutare la sua scalata al potere riuscirà ad ottenere il pontificato nel 63 avanti cristo che accade nel frattempo del non più giovanissimo cesare caio giulio cesare appartiene a una famiglia la famiglia giulia di antichissima 1 bildt a patrizia però una famiglia economicamente non florida difficoltà economiche da un po di tempo non ci sono in famiglia personalità che hanno ricoperto il consolato che la carica più alta e più potente della repubblica quindi è un personaggio indubbiamente ragguardevole con una straordinaria capacità politica letteraria di influenza di clientele eccetera ma in difficoltà sicuramente di troppo rispetto ad una scena in cui ben due potentati si sono fatti avanti e per ora vanno d'accordo ma potrebbero prima o poi collidere il celebre aneddoto di cesare che quando è pro magistrato in spagna dice all'età mia alessandro magno aveva già conquistato il mondo e io per ora non ho ancora fatto lunedì memorabile è un aneddoto probabilmente verifico che però indica in maniera molto efficace il disagio in cui quest'uomo è venuto a trovarsi dando per assodato che l'aspirazione al primato ad una posizione di primato nella repubblica è per lui un dato acquisito dato dal quale non può prescindere quindi assistiamo riconsideriamo quanto abbiamo detto fino a ora ad un fenomeno significativo è a suo modo allarmante dentro l'ordinamento costituzionale repubblicano alternanza delle magistrature il ruolo egemonico del senato eccetera ci sono però figure tali vitale spicco e di tale ambizione che puntano ad avere una posizione egemonica le due cose non vanno senz'altro d'accordo e non è una storia recente soltanto è perché già nel secolo precedente alla fine del terzo e tutto il secondo avanti cristo c'era stata un'altra grande famiglia quella degli scipioni che aveva avuto grandi meriti naturalmente nell'espansione imperiale di roma nel mediterraneo basti pensare al vittoria contro annibale e poi la conquista durissima della spagna e poi le guerre verso la parte orientale del mediterraneo insomma una famiglia che ha contato moltissimo dell'espansione imperiale è però ingombra un po tanto nella repubblica quindi già con gli scipioni si è determinato questo attrito fra aspirazioni personali molto forti di famiglie che contano anche per la ricchezza anche per le clientele anche per il prestigio militare e l'ordinamento senatorio il senato è un organo collettivo senatori non possono essere un numero infinito e quindi appartenere a questa élite dominanti di gente che ha grande carriera alle spalle che ha grandi ricchezze alle spalle appartenere a questo collegio significa guidare la repubblica collegialmente ecco perché l'attrito con le figure emergenti che debordano rispetto ai limiti ordinati dalla costituzione ecco l'attrito come si determina e dopo l'esperienza silana dopo il gesto di silva di lasciare la dittatura e vedere come funziona il sistema ecco nuove grandi figure sulla scena cesare desidera di essere una di esse è la sua direttiva non fu chiarissima dall'inizio e è vero apparteneva per legami familiari all'ambiente mariano l'ambiente di cina però come ebbe poi a dire avrebbe potuto anche scegliere un orientamento diverso quindi rappresenta questo è molto interessante per noi che cerchiamo di capire la politica romana fuori degli schemi rappresenta la tipica figura che sceglie la parte con cui schierarsi in funzione della propria carriera della propria egemonia dove può avere una base sufficientemente forte per affermarsi li crea i suoi legami politici il senato è sicuramente è l'organo di governo principale dell'antica roma la sua creazione è attribuita allo stesso romolo e in origine designava l'assemblea degli anziani il nome senato infatti deriva dal latino seneca che significa appunto vecchio anziano in un primo tempo solo i patrizi ovvero l'elite aristocratica romana potevano accedere a questa carica in seguito la missione verrà allargata a tutti i cittadini più ricchi anche il numero di senatori subirà un sensibile aumento passando dai cento delle origini fino ai 900 del periodo di giulio cesare il requisito fondamentale per entrare in senato è l'aver esercitato una magistratura cioè una carica pubblica ma occorre anche l'approvazione dei censori sono infatti questi funzionari deputati al controllo della condotta civica a stilare ogni cinque anni la lista ufficiale dei membri del senato è nella fase repubblicana che il senato diventa il fulcro della vita pubblica assumendo di fatto il controllo dello stato e della sua stabilità i suoi compiti vanno dal controllo delle cariche pubbliche alla discussione dei progetti di legge dalla gestione finanziaria alla disciplina del culto religioso anche l'attività militare e la politica estera sono sotto la sua autorità i senatori organizzano le operazioni belliche stipulano gli accordi di pace e ratificano le dichiarazioni di guerra durante le guerre civili del primo secolo avanti cristo il senato perde il ruolo di garante dell'unita e della concordia politica da espressione degli interessi comuni diventerà un baluardo della fazione conservatrice gli optimates segnando così il proprio declino e quello della repubblica qual è lo scenario sociale in cui queste figure si muovono ormai è uno scenario profondamente modificato rispetto alla città stato dei tempi degli scipioni da una parte c'è stato un fenomeno di dilatazione geografica del concetto città di roma noi pensiamo a roma come a una città stato come in origine essa è effettivamente ma con la gestione e sapiente della cittadinanza della dell'allargamento della cittadinanza roma lentamente si dilata cittadinanza romana cittadinanza di diritto latino poi il lazio assume la cittadinanza romana gli italici che sono stati conquistati con guerre durissime con cui roma ha lentamente conquistato la penisola premono per avere la cittadinanza anch'essi i greci avevano posto la questione il tribuno druso l'aveva anche drammaticamente riproposta e ci aveva lasciato la vita sila ha sconfitto sul campo gli italici però al termine della guerra sociale nell 88 la cittadinanza romana è stata concessa a tutti gli italici a sud del po quindi roma è l'italia a sud del po fino alla punta estrema quando si devono fare le elezioni politiche le chiamiamo così si eleggono i magistrati più importanti consoli di edili i pretori si va a votare a roma ed a tutti i vari municipi d'italia ci si muove a proprie spese insediandosi temporaneamente nella capitale per esercitare il diritto di voto conquistare il consenso di questo grande elettorato non è un problema da poco la plebe urbana di roma però conta moltissimo mentre gli italici possono decidere di non scomodarsi ogni volta a venire a votare a roma la plebe urbana come viene chiamata base sociale dei capi populares sta lì e vive sostanzialmente con sussidi elargizioni di grano feste insomma non è che sia un ceto particolarmente produttivo è un ceto abbastanza parassitario che pesa moltissimo proprio come una massa critica addosso alle istituzioni anche potremmo dire addosso al senato allearsi con la plebe urbana significa avere una massa di manovra elettorale molto importante da mettere a frutto magari anche con fortissimo intervento economico le elezioni costano moltissimo a roma è un modello che si è protratto nei millenni questo è il quadro diciamo così politico elettorale poi c'è un altro elemento c'è un altro elemento del quale cesare capisce presto l'importanza studiando il suo futuro avversario ma per ora interlocutore più forte cioè pompeo quest altro elemento sono le legioni la massa militare a massa militare è costituita da dei cittadini che chiamati alle armi ci restano per anni e anni un servizio che dura moltissimo che diventano gli interlocutori diretti dei capi militari i capi militari che li portano alla conquista per esempio il caso di pompeo dell'oriente il favoloso oriente che sia aperto a roma grazie a questo grande capo militare per ciò detto pompeo magno le legioni vedono questi capi in particolare nel caso di pompeo il loro interlocutore diretto quello che con terminologia moderna giustamente famosa potremmo chiamare il capo carismatico la mediazione politica conta di meno per loro conta il rapporto diretto con il capo questa massa militare che è fatta di cittadini soldati naturalmente diventa col tempo sempre più importante rispetto alla plebe urbana più o meno parassitaria che sta nella capitale ed è la massa di manovra elettorale usata spregiudicatamente da dalle varie parti politiche ma tendenzialmente legata ai populares e che vede in alcuni dei tribuni della plebe i propri naturali rappresentanti cesare capisce presto che accanto alla consuetudine alla routine elettorale in cui è bravissimo straordinariamente capace c'è poi quell altro soggetto quello della massa militare con cui stabilire un contatto tale da creare una propria autonoma forza all'interno dello scontro politico e quindi quando si reca in spagna da pro magistrato dopo aver ricoperto la pretura lui cercherà di stabilire questo rapporto con le truppe che gli saranno affidate che sono poca cosa rispetto alle legioni pompeiane naturalmente ma anche cerca di stabilire dei contatti di carattere clientelare cioè con le élite locali ciò che pompeo aveva saputo fare magnificamente il suo colpo di genio potremmo dire quando si trova in una posizione minore rispetto ai due potentati crasso il pompeo suo colpo di genio e di puntare ad una carica l'unica carica vitalizia ma elettiva della repubblica romana che è pontefice massimo pontefice massimo viene eletto con una battaglia elettorale estremamente dura ed è tale finché non muore quindi conquistare la carica il pontefice massimo significa avere una posizione stabile nell'ordinamento cesare era considerato e certamente lo era molto vicino simpatizzante per la filosofia epicurea a roma l'epicureismo si era diffuso come li racconta cicerone nelle tuscolane in maniera straordinariamente pervasiva grazie a dei divulgatori del pensiero di epicuro che conosciamo di nome di tale si chiamava ma fini o di lui abbiamo soltanto questo cenno che fa cicerone o poco più cicerone dice hanno invaso tutta l'italia dp curerà un po allarmista ma indubbiamente era un orientamento filosofico incongruo rispetto alla centralità della politica caratteristica della repubblica romana per un personaggio che ha aspirazioni anche modeste la politica e l'attività principale è il mestiere fondamentale quindi bisogna avere anche quattrini una base economica legami quella è il mestiere quello è il mestiere di un vero cittadino romano è la l'epicureismo suggerisce di tenersi lontano dalla politica di non impegnarsi vivi nascosto il celebre motto attribuito ad epicuro e oltre a ciò ritiene che gli dei probabilmente esistono anzi ci saranno certamente da qualche parte ma si disinteressano delle cose umane la vicenda umana e un fluire di atomi materialisticamente aggregati disgregati l'anima è essa stessa mortale lui se c'è una filosofia che è lontana dal mestiere di cittadino ed al mestiere di pontefice massimo è certamente l'epicureismo per cui è interessante osservare questa sorta di scissione nella persona per cui il cesare intellettuale raffinatissimo forse il più grande scrittore di lingua latina che sia mai stato e di simpatia e figure e e al tempo stesso in quanto votato alla politica ha votato a contare nella repubblica possibilmente in una posizione egemonica egli e politico di mestiere e pontefici massimo poteva fermarsi per la più alta carica religiosa che non è soltanto strettamente religiosa perché il pontefice massimo come sappiamo da tantissime testimonianze episodi eccetera può paralizzare la vita politica può mettere dei veti fondamentali rispetto all'attività dei consoli ha un peso estremamente significativo direttamente politico quando egli si presentò alle elezioni per il pontificato massimo raccontano le biografie su e si congedò da sua madre dicendo o torno pontefice o torno in galera nel senso che si era talmente indebitato per la campagna elettorale da doversi poi difendere da accuse gravi per debiti non pagati vinse le elezioni d'altra parte le elezioni si vincono a tavolino prima di farle per cui egli aveva molte carte nelle sue mani c'è un romanzo bellissimo purtroppo incompiuto di uberto express three gli affari del signor giulio cesare che racconta molto da vicino e senza peli sulla lingua esattamente questo aspetto della figura di cesare all'opera come politico l'epicureismo è una delle grandi scuole filosofiche dell'età ellenistica nasce in grecia verso la fine del iv secolo avanti cristo pochi anni dopo la morte di alessandro magno secondo il suo fondatore epicuro di samo la filosofia ha come unico scopo liberare l'uomo dai suoi mali e condurlo verso la felicità [Musica] per raggiungere questo obiettivo è necessario sconfiggere le quattro grandi paure che affliggono l'animo umano la paura degli dei della morte del dolore e dell'infelicità la medicina di epicuro il cosiddetto quadri farmaco è basata su una concezione materialista dell'universo in cui le divinità sono indifferenti alle vicende terrene il timore della morte è irrazionale e la felicità è semplicemente la mancanza di dolore il compito dell'uomo saggio è giungere a una condizione di serenità detta atarassia in cui vi è assenza di ogni turbamento un atteggiamento che si traduce in un'esistenza appartata lontana dalle forti passioni ed alla partecipazione politica [Musica] questa visione individualistica e isolata della vita non sembra in armonia con gli ideali della roma repubblicana nella quale l'impegno politico e l'interesse comune sono tenuti in grande considerazione tuttavia nel corso del primo secolo avanti cristo il pensiero epicureo si diffonde con successo nella capitale ciò avviene grazie all'opera di alcuni studiosi greci seguaci di epicuro che si trasferiscono in italia portando con loro le idee del maestro l'affermazione delle pitture ismo si deve soprattutto all'opera di lucrezio il suo poema de rerum natura traduce in raffinati versi il pensiero del filosofo greco e costituisce il punto più alto della scuola epicurea latina cosa accade quando lui è pontefice massimo e pretore designato anno 63 ormai avanti cristo sono passati vari anni come vediamo dall'anno 70 in cui crasso il pompeo sono i padroni della repubblica nell'anno 63 e console marco tullio cicerone suo collega si chiama antonio non è ovviamente il futuro trio come è riuscito cicerone a farsi eleggere in una campagna elettorale combattutissima appoggiato da uno schieramento di optimates è riuscito contro lucio sergio catilina catilina e un nobile decaduto che ha in mente qualcosa che potrebbero assomigliare a una rivoluzione degli ordinamenti politico sociali romani di lui sappiamo quello che ci dicono i suoi nemici i suoi avversari sappiamo quello che dice cicerone nelle catilinarie per l'appunto che sono conservate l'idea che ci si può fare di questo personaggio finito tragicamente come diremo è che metteva insieme i resti di una serie di esperimenti falliti un po di avanzi di legionari sillani scontenti avanzi della guerra sociale reduci da campagne persino di quelle connesse alla guerra contro spartaco come suoi seguaci diciamo così retro scenici ma naturalmente il suo impegno era vincere le elezioni diventare console dopo un primo insuccesso egli si attrezza meglio e nell'anno 64 si vota per eleggere i consoli dell'anno 63 la sua candidatura è molto forte dietro le quinte è probabile che lo appoggiasse anche crasso anche cesare poi essere ha fatto scrivere in lungo in largo che lui con la congiura non c'entrava per nulla però prima del fallimento della congiura uno si chiede sempre potrebbe vincere quindi non è male restare da quella parte siccome è pericoloso come candidato e gli italici sono venuti in massa a votare per lui gli auguri ruolo religioso importantissimo subordinato a quello di pontefice massimo fanno sapere che gli auspici non sono favorevoli quindi non si può votare quindi tu questi italici se ne tornano nelle loro sedi le lezioni sono rinviate e quando si vota da capo non c'è più questa massa di sostenitori pronta a rifare il viaggio per venire a roma a votare per caterina cicerone vince caterina e sconfitto ancora una volta quando ormai era convinto di farcela e a quel punto secondo le narrazioni a noi giunte concepisce una presa del potere per via violenta rivoluzionaria creandosi delle alleanze retroscena che anche cerca di allearsi con dei galli che poi lo tradiranno ammazzare i consoli in carica ammazzando i consoli in carica si decapita lo stato a repubblica è in quel disordine si prende il potere grosso modo questo è il suo disegno questo disegno fallisce cicerone verso la fine dell'anno 63 alle prove abilmente catturate della congiura aggredisce in senato ex abrupto catilina con la famosa prima catilinaria pronunciata di getto con un oratorio irruenta efficacissima in parte riprodotta in quella che noi chiamiamo la prima catilinaria da lui ovviamente riscritta dopo porterà in tour e avendo le prove di questo reato gravissimo che è stato concepito e stava per essere perpetrato ha avuto l'accortezza di emettere guardie davanti a casa sua per impedire che l'attentato nei suoi confronti venisse attuato avendo le prove in mano porta davanti al senato l'accusa di eversione e la proposta di esecuzione immediata dei congiurati cioè senato siede in quel momento in una funzione giudiziaria cosa rarissima dinanzi all'alto tradimento che è appunto la uccisione dei magistrati più importanti della repubblica questa procedura può avere un senso e id i pareri sono divisi noi abbiamo notizie di questo drammatico dibattito che si è svolto in senato in cui cesare è intervenuto in cui è intervenuto catone un po più giovane di cesare di età interviene cicerone stesso è la proposta di esecuzione capitale immediata sta per essere approvata a cesare si oppone si oppone e del pontefice massimo mentre parla in senato contro le esecuzioni capitali dei cattivi nari e ha svolto una tesi piuttosto originale che cioè visto che probabilmente l'anima immortale come alcuni ritengono quella eco della sua adesione diciamo così filosofica l'epicureismo uccidere e meno punitivo che mettere in carcere a vita e quindi teniamo lì in vita perché così veramente li puniamo e sta per ottenere il consenso con questa formulazione non perché ci fosse una setta epicurea diffusa ma perché arretrare dinanzi a un passo così grave tutto sommato attrae la maggioranza dei senatori interviene a quel punto catone con un discorso fortissimo sprezzante nei confronti di cesare dei confronti dei senatori che sono titubanti la maggioranza approva l'esecuzione capitale e per ordine del console in carica i congiurati vengono strangolati nel carcere mamertino e cicerone dall'annuncio al popolo con la famosa espressione vix erunt sono morti erano vivi lavoro sono morti cesare n'è uscito con qualche ammaccatura da questa vicenda crasso non ha problemi perché classe l'uomo più ricco di roma tutti sanno che ha flirtato con i catilinari ma nessuno mai oserebbe toccarlo pompeo fuori del pomerio cia le sue legioni che vogliono i premi adeguati per essere stati i conquistatori dell'oriente cicerone trionfa cile non è il salvatore della patria e daccapo acclamato pater patrie secondo romolo ha rifondato roma se un po montato la testa perché naturalmente capeggiava uno schieramento non avere tutto merito suo uno schieramento che ha ritenuto che schiacciando catilina si dava un colpo mortale ai populares e quindi allo stesso cesare fosse o meno coinvolto nella congiura lui una situazione termina di lì a poco con la battaglia di pistoia perché catilina è fuggito da roma con i suoi seguaci e l'altro console antonio lo ha affrontato sul campo di battaglia a pistoia sono stati ammazzati tutti catilina muore in battaglia dopo questa vicenda si può dire la parte degli orti mates si considera al sicuro sorpresa nell'anno 60 quindi siamo a ridosso 62 si è svolta la battaglia di pistoia nell'anno 60 pompeo insoddisfatto di come il senato non dà soddisfazione alle sue legioni crasso desideroso di mettersi al sicuro dopo la rischiosa parte che ha giocato nella vicenda catilinaria cesare che tutto sommato ha avuto un ruolo protagonistico nella battaglia in senato si mettono d'accordo privatamente quindi è un patto privato chiamato volgarmente primo triunvirato e stabiliscono di allearsi anziché farsi la guerra il primo triunvirato cosiddetto patto privato tra tre potentati nell'anno 60 decide quali saranno i consoli dell'anno prossimo come sarà gestito il potere quali province saranno date ai contraenti quali leggi e consoni eletti faranno passare uno potrebbe dire ma prima delle elezioni già si sa come andrà a finire già si sa come era finita nel senso che è la forza diciamo suadente della manipolazione del consenso è un fatto centrale nella repubblica ormai potremmo dire agonizzante e viene eletto cesare consone per il 59 suo collega perché non si può avere tutti e due i posti e marco calpurnio bibulo uomo legatissimo a gli ottimati un po ottuso essere un genio non solo politico ma anche intellettualmente versatile più diversi campi bibulo è un vecchio signore tradizionalista abituato a dire no ogni volta che i popolare si dicono sì piume quindi una convivenza difficile il compito di cesare la console è innanzitutto di varare delle leggi leggi agrarie che favoriranno i veterani di pompeo con assegnazioni di terre deducendo colonie di veterani pompeiani è un'operazione di destra o di sinistra nel popolare o ultimate è un'operazione a favore del grande soggetto sociale che sono le regioni cioè se lei ha capito benissimo che è quello l'interlocutore contro la sua legislazione agraria bibulo mette il veto cioè si crea una situazione paradossale dal punto di vista costituzionale cioè un console mette il veto all'attività dell'altro console come sappiamo chi con soli sono due perché devono agire collegialmente no quando fu istituito il consolato alla fine della monarchia cacciato tarquinio si disse lo racconta tito livio efficacemente che il potere dei consoli era analogo a quello dei re però durava un anno soltanto e perché fosse minore il peso erano due e quindi la collegialità delle decisioni dei due massimi magistrati è un cardine della repubblica cesare si disinteressa di questo contrasto che bibulo scatena in tutte le forme auspici negativi il diritto di veto bibulo si ritira in casa e non collabora più quasi a creare una situazione di crisi istituzionale irreparabile cioè sarebbe avanti lo stesso quindi il consolato di cesare l'anno 59 è già ai limiti della legalità se non oltre la legalità ma alle spalle c'è l'appoggio degli altri due i quali hanno un soggetto potentissimo dietro di sé cioè le regioni e quindi senato abbozza come si suol dire deve accettare una situazione di paradosso costituzionale e istituzionale ai limiti della tollerabilità per giunta e qui essere diventa un certo senso l'uomo che vince la partita rispetto agli altri due si fa attribuire come governo provinciale per cinque anni la gallia abbiamo la gallia cisalpina alla gallia narbonese che sono già province romane è però c'è la gallia cometa cioè la gallia non ancora sotto il controllo romano che egli si propone di conquistare quindi qual è il colpo di genio che cinicamente lui mette in atto determinare una situazione di conflitto prolungato che richiede una grande massa militare per essere attuato che gli determinerà l'analogo di ciò che pompeo ha avuto nella sua lunghissima carriera di conquistatore a oriente e deve creare il casus belli quindi entra in scena potremmo dire come pro magistrato delle gallie col proposito di determinare un conflitto per prolungare una guerra durissima con le tribù galli che ed estendere la presenza romana sull'intera gallia anche quella cometa anche quella belgica che appunto di fronte alle isole britanniche noi abbiamo un libro straordinario dei più bei libri della letteratura latina cioè de bello gallico che tempo si leggeva a scuola nelle scuole medie inferiori senza apprezzarne l'importanza e rilievo la lingua raffinata e soprattutto la falsità potrebbero dire la falsità nel senso del carattere spiccatamente propagandistico dalla prima all'ultima pagina di quest'opera che mira sostanzialmente a convincere l'elite dirigente che legge della necessità di quel conflitto delle ragioni di esso della giustezza della condotta bellica di cesare e quindi è un'arma così come lo sono le legioni come nascono questi commentari de bello gallico sono lo sviluppo letterario delle relazioni al senato che in quanto pro magistrato e gli invia regolarmente per spiegare i successi che via via sta conseguendo e chiedendo a ridosso le suppliche azione cioè delle solenni forme di riconoscimento da parte del senato dei successi che lui ha ottenuto si ricorda spesso il fatto che gli eletti sono stati all'inizio lo strumento dello scatenamento di questa guerra meno si ricorda il fatto che l'operazione ha comportato vere e proprie forme di genocidio popolazioni centinaia di migliaia di persone sono state massacrate gli usi petite teri al punto tale da determinare a roma una reazione contro cesare da parte dei suoi avversari quel personaggio abbiamo ricordato prima catone che continua la sua azione di contrasto rispetto ai tre e in particolare rispetto cesare buone davanti al senato la questione bisogna consegnare cesare ai galli come criminale perché ha commesso un ingiustificabile genocidio che determina anche una conflittualità sempre più aspra è un'immagine della repubblica romana tutt'altro che limpida è una richiesta estrema che non passa in senato però un campanello d'allarme i cinque anni trascorrono nel frattempo i rapporti tra i tre si deteriorano c'è stato un patto che poteva entrare in crisi questo patto viene rinnovato negli anno 55 59 era console cesare il 58 è andato in gallia come pro magistrato come proconsole 55 si riuniscono a lucca cesare e pompeo e crasso e rinnovano il patto il patto di lucca comporta un alleanza familiare tra cesare e pompeo pompeo sposa la figlia di cesare l'unica figlia che lui aveva e si stabilisce che saranno da capo consoli pompeo e crasso insieme il secondo consolato di pompeo e crasso che puntualmente vengono eletti tutti e due sancisce potremmo dire il patto di lucca lo corona lo completa crasso si rende conto che non basta dominare a roma oa vere ricchezze sterminate bisogna avere anche un potere militare e mentre pompeo ha già fatto la sua parte il conquistatore dell'oriente cesare sta facendo del suo meglio in gallia e gli si fa attribuire come pro magistratura dopo la conclusione del consolato della provincia di siria la provincia di siria era stata inventata da pompeo nell'anno 67 la dinastia dei seleucidi era finita nel nulla lui aveva conquistato senza colpo ferire antiochia era entrato a gerusalemme aveva violato il tempio cosa ragione per cui gli ebrei lo hanno sempre detestato è però la siria era l'avamposto militare della repubblica imperiale romana nei confronti di un regno l'ultimo grande legno e le nistico regno ellenistico potremmo dire cioè quello dei party che incombono sul confine orientale in particolare sulla provincia di siria per che incombono non vogliamo dilungarci su questo ma è certamente importante dire che lo strato e legni zato delle città nella provincia di siria e molto superficiale la popolazione indigena diciamo così subisce la presenza romana e guarda ai party come possibili liberatori i parti lo sanno benissimo e quindi le loro incursioni nella provincia sono spesso micidiali quindi è il punto più debole della linea difensiva romana quel confine grasso si avventura da pro magistrato in una impresa che si risolverà in una catastrofe la battaglia di carrè lui si inoltra nella mesopotamia con le legioni un suo ufficiale e quel caio cassio longino che poi ammazzerà cesare nel 44 brillante ufficiale bravissimo che si salva in questo disastro crasso viene catturato decapitato e diciamo la fine tremenda che lui fa è anche il segno potremmo dire che ormai la scena politica si riduce a due grandi rivali che sono cesare e pompeo cesare capisce benissimo che la scomparsa di crasso non gli giova perché accelera lo scontro è si avvicina al momento dello scontro sapendo di essere una posizione difficile ha rinnovato il comando galli così di è stato protratto ma per salvarsi dai suoi avversari i quali sono pronti non appena uscirà di carica a portarlo in tribunale per le malversazioni al tempo del suo primo consolato che lo vorrebbero portare in tribunale per tante altre ragioni tra l'altro quelle che catone ha portato davanti al senato per sottrarsi a questa probabilissima persecuzione giudiziaria deve farsi eleggere console la magistratura lo metterebbe al riparo e però se lascia la pro magistratura per venire a farsi la campagna elettorale a roma e vulnerabile cioè nello spazio tra l'uscita di scena come pro magistrato e l'entrata in carica eventuale come console c'è un interstizio un intervallo in cui i suoi avversari lo porteranno in tribunale e lui sa che va a finire male perché i tribunali sono funzionano con altrettanta oggettività quanto le elezioni il corpo elettorale e quindi la sua battaglia col senato attraverso una trattativa che conosciamo abbastanza bene e mirante ad ottenere la possibilità ha la facoltà di farsi eleggere in assenza cioè lui sta in gallia continua a serbare la posizione di pro magistrato e si candida alle elezioni protetto dalla posizione di pro magistrato quindi chiede di essere candidato pur non essendo a roma pur non avendo deposto la carica diplomati strato esponendosi quindi al rischio che abbiamo appena detto e su questa battaglia che si arriva alla guerra civile in questa battaglia pompeo naturalmente non si sporca le mani ai suoi uomini in senato la potente famiglia dei cloud di marcelli naturalmente catone più tutta una serie di figure di secondo piano il senato è dominato dai grandi personaggi però poi ha anche le masse di manovra naturalmente e sigla il suo tempo lo aveva decimato ma poi si è riformato è un organismo duro a morire il senato e ha uno spirito di corpo fortissimo quindi quando si nel 49 cesare ci rende conto che la situazione gli è sfavorevole perché questa sua richiesta di poter essere candidato in assenza non avrà sbocchi si vede per giunta richiedere due legioni da pompeo il quale fa deliberare dal senato che gli venga affidata alla campagna contro i party per vendicare l'uccisione di crasso per riprendere la campagna partica quindi ha bisogno di legioni e quindi la campagna gallica ormai è finita ci essere riuscito a schiacciare tutte le varie rivolte vercingetorige l'assedio di alesia e quindi gli può dare due regioni cioè se ne sta al gioco gli molla due regioni quindi si trova in una posizione anche militarmente inferiore il punto di non ritorno dopo che questa estenuante trattativa è arrivata sul binario morto e all'inizio di gennaio di primi giorni di gennaio dell'anno 49 avanti cristo il senato si riunisce decide la spartizione delle province attribuisce le provincia ai magistrati dell'anno 50 cesare l'utente escluso completamente da questa operazione è caccia brutalmente antonio che in quel momento e tribuno della plebe dalla possibilità esclude completamente dalla possibilità di contestare queste decisioni del senato noi abbiamo un racconto splendido faziosissimo naturalmente ma molto efficace di questa vicenda nel primo commentario cesariano della guerra civile e un testo memorabile dove c'è se le descrive questa furia questa visita dei dirigenti dei dei leader della pars attivate mentre pompe non sta a guardare che si spartiscono le cariche il potere le legioni le regioni le province e descrivere tutto questo subito dopo aggiunge la fuga da roma dei tribuni della plebe che sono stati maltrattati fisicamente aggrediti che lui esibisce davanti alle legioni mentre sta sul confine tra la provincia della galles alpina e l'italia il comizio che lui fa mostrando alle truppe i due tribuni ancora coperti di sangue di lividi di tunica e strappate una scena di grande efficacia patetica e quindi la decisione che emerge per acclamazione e invadere l'italia il passaggio del rubicone confine tra la provincia nella gallia cisalpina e roma cioè l'italia rimini è la prima tappa a quel punto la guerra civile a inizio è scoppiata nel momento stesso in cui cesare ha violato con le sue truppe la città di roma cioè rimini la situazione che si determina è velocissima nelle sue dinamiche e anche a suo modo sconcertante la decisione che prende la parte senatoria su suggerimento di pompeo e di lasciare roma perché si immagina che la bravura tattica di cesare la velocità che ci sarà sempre avuto nei suoi spostamenti quando durante le campagne galli che è tale che in brevissimo può essere a roma e pompeo non gradisce di rifare la scena da sillana per cui la guerra civile si combatte sul suolo della città di roma e sceglie una linea che a lui sembra sicura ma in realtà è avventuristica trasportare il senato le magistrature le regioni se stesso in oriente imbarcarsi a brindisi sbarcare in epiro rafforzarsi lì e lì aspettare cesare per uno scontro militare definitivo perché lo ha fatto ma tanto perché evidentemente non riteneva agevole affrontare e cesare con le legioni di cui disponeva in quel momento in italia probabilmente in una posizione per dente o comunque problematica e poi perché era sicurissimo della sua forza in oriente dove aveva le sue clientele da sempre dove non soltanto il tessuto diciamo così militare ma anche quello clientelare delle élite locali era a suo favore senato e sconcertato naturalmente perché senato la prima volta nella storia di roma che si sposta lascia la sua sede naturale va un'altra parte della repubblica imperiale e in gran parte lo segue gran parte lo segue alcuni restano a roma a cui cicerone cicerone e angoscia tissimo cicerone ha sognato di essere l'uomo chiave in questa vicenda della guerra civile ma in realtà è stato una pedina da un certo punto in avanti è una testa politica lucidissima non ha alcuna stima di pompeo in lettere private che noi abbiamo di lui scrive chiaramente questo uomo cioè pompeo vuol fare come sigla vuol fare anche lui le sue prescrizioni quindi non è il garante della legalità repubblicana è un potentato militare che vuole fare il princeps in repubblica cioè vuole essere quello che era stato sigla e che ha sempre desiderato durante tutta la sua carriera di è chiarissimo però non si fida neanche di cesare cesare invece per parte sua loeb adulando dimostra grande considerazione gli manda lettere cesare gliele ha fatte recapitare con l'aiuto di due suoi fedelissimi occhio e balbo si chiamavano erano i suoi agente singh regole su come si diceva manutengoli fedelissimi abilissimi a roma durante i molti anni della sua assenza in gallia e occhio e balbo trasmettono questo messaggio a cicerone gli dicono senza il tuo consiglio lui non farà una mossa aspettano qui a roma perché sarà il suo consigliere cosa naturalmente inverosimile alla fine anche cicerone decide ricattato un po da amici izzy persone che gli erano sempre state vicine si decide anche lui e imbarcarsi a brindisi è unirsi al campo di pompeo cesare al seguito da grandissimo stratega qual era una linea politica militare assolutamente imprevedibile per tutti tutti si aspettavano che abboccasse corresse anche lui in epiro a combattere invece va in spagna che apparentemente una mossa sconcertante non senza essere passato da roma impadronendosi dell'erario quindi si rafforza economicamente prendendosi il tesoro dello stato si fa nominare dittatore dictator che la una carica come abbiamo detto prevista dall'ordinamento romano non c'è il console che dovrebbe nominare che cosa che se ne sono andati con pompeo allora si fa nominare il suo magistero equity comunque è dictator e come tale al diritto di mettere le mani sul denaro dello stato si reca a marsiglia e poi in spagna per liquidare le forze pompeiane che stanno lì perché lui sa benissimo e questo era il disegno di pompeo che se si imbarca brindisi sarà stretto in una tenaglia fra i pompeiani che vengono dalla spagna ai pompeiani che stanno in grecia quindi passa mesi a sistemare l'occidente dopo di che ritorna a roma si imbarca brindisi e nel momento più favorevole possibile anche dal punto di vista climatico quando nessuno si aspettava che sbarcasse sulla costa adriatica antistante a rischio anche di naufragi di danni irreparabili piomba in epiro e poi in tessaglia e lì la battaglia campale di farsalo agosto nell'anno 48 è uno dei modelli potremmo dire della sua capacità militare di capovolgere una situazione sfavorevole tale sul campo e diventa lui favorevole tanto che i morti della parte avversa sono infinitamente di più dei pochi delle sue truppe qui siamo a 4 anni dalla sua morte che avverrà il 15 marzo del 44 questi anni sono quasi per intero dedicati ad una guerra civile interminabile una lunghissima guerra civile di solito la si racconta sapendo come va a finire purtroppo nel raccontare la storia è inevitabile a questo punto di vista perché il narratore saggia l'epilogo poteva andare a finire diversamente prima tappa dagli esiti imprevedibili pompeo fugge in egitto perché sa che l'egitto è roba sua i sovrani che si sono succeduti sono stati tutti i suoi clienti lui ha fatto riportare sul trono un certo tolomeo ha un'età che era un soggetto piuttosto deplorevole ma comunque nulla a farlo ha piazzato le sue truppe sono rimaste in egitto per sorreggere quest'uomo quindi è convinto di andare al sicuro nonostante la sconfitta tremenda farsalo la sorpresa qual è che approda in un egitto dilaniato da un conflitto dinastico e i notabili che contano intorno al re che un bambino un fanciullo pensano che ammazzando pompeo possono avere con cesare un buon rapporto o comunque un rapporto privilegiato e quindi attraggono catturano pompeo che sbarcano in una barchetta al porto di alessandria lo trafiggono lode capitano e quando cesare che si è lanciata all'inseguimento di proprio con pochissime truppe approda ad alessandria gli mostrano la testa di pompeo e cesare mostra di essere commosso ma crisi di pianto mi è stata rimproverata questa scena come di estrema falsità ipocrisia forse c'è anche un elemento di verità di patetismo vero in questa scena tutto sommato quest'uomo non era stato anche a lui molto vicino e soprattutto cesare capisce che se questi re clienti sono capaci di questo sono capaci anche nei suoi confronti di colpi di mano o di pugnalate alla schiena cosa che puntualmente accade lui viene imbottigliato nel palazzo reale di alessandria dallo stesso gruppo intorno al tolomeo tredicesimo fanciullo che ha liquidato pompeo e soltanto con tutta la sua abilità tattica a rischio di perdere completamente la sua flotta incendiandola dall'altro del palazzo e provocando l'incendio di una parte della città di alessandria in attesa di aiuti che tardano ad arrivare lui riesce a cavarsela da un assedio che poteva essere mortale alessandria è stato un punto di non ritorno napoleone ha scritto quando era ormai a sant'elena un breve libro sulle guerre di giulio cesare in cui rimprovera cesare l'imprudenza di alessandria dopo quella inopinata vittoria che poteva essere la sua tomba cesare aiutato dagli ebrei che sono accorsi in suo aiuto amici di cesare nemici di pompeo risale l'oriente rientra in italia sbarca brindisi e si trova da capo davanti una sedizione repubblicana quel catone che abbiamo più volte rubinato si è spostato nel nord africa e li organizza con l'aiuto dei re locali clienti dei romani l'ultima resistenza repubblicana la battaglia di thapsos e siamo nell'anno 46 cioè cesare passa da roma si fa a rinnovare la dittatura ma è costretto a proseguire la guerra civile che come vediamo si svolge in tutto il teatro mediterraneo a base di thapsos è stata durissima catone dopo la sconfitta si è suicidato cesare ha riordinato i rapporti diciamo così di clientela e di dipendenza da roma in quell'area geografica ma quando torna a roma si trova dinanzi all'ultima è durissima secessione quella dei figli di pompeo che in spagna avevano una base formidabile di clientele e che hanno organizzato la guerriglia quindi ancora una volta si mette in strada barca i pirenei arriva a monda e nella battaglia di monda si gioca tutto la materia di monda sta per volgere a suo sfavore e siamo nell'aprile del 45 si impegna personalmente prende in mano le insegne le aquile eccetera e la battaglia volgere a suo favore torna finalmente e questa volta non ci sono più teatri di guerra fa l'errore di trionfare anche sui pompeiani sconfitti in spagna cosa che non gli verrà mai perdonata dai suoi avversari dove a trionfare sui galli sull'egitto ma non su i suoi concittadini questo è il punto critico un errore tattico non da poco che lui commette la vicenda finale è presto detta è una vicenda in cui dal suo stesso entourage in particolare da antonio parte l'iniziativa pericolosissima di proporgli di assumere il ruolo di rex la corona durante i lupercali antonio gli offre questa corona lui la respinge la fa mettere sulla statua di giove però ormai nonostante la sua abile capacità clemenza cesaris di catturare gli avversari perdono politicamente generosissimo nonostante tutto questo serpeggia ormai nel elite senatorio in una parte di essa l'idea che quest'uomo facendosi attribuire dopo monda la dittatura a vita e quindi ormai una forma di potere monarchico diventa colui che affosserà anzi ha già affossato la repubblica ecco come nasce dal suo stesso ambiente la congiura marco giunio bruto suo figlioccio suo protetto e caio casio longino che abbiamo ricordato prima nella campagna di crasso contro i parti sono i protagonisti di una congiura per la quale più o meno i leader cicerone incluso sono coinvolti congiura che ha un risultato immediato quasi prevedibile tante pugnalate sullo stesso corpo una almeno è mortale e cesare rotola ai piedi della statua di pompeo ma il problema politico rimane tutto aperto i congiurati hanno perso il giorno dopo quando si sono accorti che la gran parte è l'opinione pubblica della plebe urbana che le legioni che una parte consistente del senato non accettano il nuovo stato di cose ea quel punto si apre un nuovo ciclo di guerre civili che si concluderà nel 31 avanti cristo è stato detto che cesare non ha fondato la monarchia uno storico inglese ha scritto ha fatto tutto ma certo non ha liquidato la repubblica questa fase sembra un po strana se uno considera che l'attentato è stato fatto perché gli si rimproverava di voler essere dittatore a vita ma l'errore compiuto sul piano istituzionale da cesare è stato di dilatare all'infinito una magistratura straordinaria alla dittatura cioè non ha saputo scegliere la forma costituzionale per far uscire dalla crisi repubblicana ci mette molto tempo la repubblica romana per diventare principato cioè governo del princeps il capolavoro lo farà ottaviano il quale fingerà di restaurare la repubblica nell'anno 27 avanti cristo arrogando a sé il ruolo di princeps realizzando dunque quel potere personale fondato sulle regioni che ci essere ha intuito ma dandogli una veste repubblicanaComprehension Test: Superbook – Revelation Theme: Forgiveness, Salvation, and God’s Victory 🔹 Remembering (Items 1–4) What mistake did Chris make at the beginning of the episode? A. He lied to his parents B. He started a fire accidentally C. He broke a valuable item D. He skipped school Answer: B Who are the three main characters in the episode? A. Peter, Mary, and John B. Chris, Joy, and Gizmo C. David, Sarah, and Eli D. Paul, Ruth, and Micah Answer: B Where does Superbook transport the children? A. To the Garden of Eden B. To the time of Jesus’ birth C. To the end times D. To the Exodus Answer: C Who receives visions in heaven during the episode? A. Moses B. Apostle Paul C. Apostle John D. King Solomon Answer: C 🔹 Understanding (Items 5–8) Why does Chris believe his mistake is unforgivable? A. He was punished severely B. He hurt someone intentionally C. He fears the consequences are too great D. He doesn’t understand forgiveness Answer: C What does the descent of the New Jerusalem symbolize? A. The end of all prophecy B. The beginning of war C. Eternal life and God’s promise D. The destruction of the earth Answer: C What lesson does Chris learn from his father? A. That mistakes are permanent B. That forgiveness is earned C. That God’s mercy is unconditional D. That punishment is necessary Answer: C What theme is emphasized throughout the episode? A. Justice and revenge B. Power and control C. Forgiveness and salvation D. Wealth and prosperity Answer: C 🔹 Applying (Items 9–12) If a student feels guilty for a mistake, what lesson from the episode could help them? A. Avoid responsibility B. Seek revenge C. Trust in God’s forgiveness D. Hide the truth Answer: C How might Joy’s experience in heaven help her understand God’s plan? A. She sees the consequences of sin B. She learns about ancient history C. She witnesses God’s ultimate victory D. She meets famous prophets Answer: C What action could reflect the message of salvation in daily life? A. Ignoring others’ mistakes B. Offering forgiveness to someone who hurt you C. Avoiding difficult conversations D. Seeking personal gain Answer: B How could Gizmo’s role support the group’s understanding of Revelation? A. By distracting them with jokes B. By translating the visions C. By guiding them through spiritual truths D. By fixing technical problems Answer: C 🔹 Analyzing (Items 13–16) What contrast is shown between Chris’s fear and the final message of the episode? A. Fear leads to punishment; forgiveness leads to peace B. Fear is stronger than faith C. Mistakes are never forgiven D. God ignores human emotions Answer: A Why is the battle between angels and Satan significant? A. It shows the power of war B. It represents the struggle for heaven C. It symbolizes the victory of good over evil D. It predicts future disasters Answer: C What does the episode suggest about the nature of sin? A. It is always punished B. It can be forgiven through God’s mercy C. It is inherited D. It defines a person’s worth Answer: B How do Chris’s emotions evolve throughout the episode? A. From joy to anger B. From guilt to hope C. From confusion to pride D. From fear to rebellion Answer: B 🔹 Evaluating (Items 17–18) Do you agree that no sin is too great for God’s forgiveness? Why might this be important for viewers? A. Yes, it teaches hope and redemption B. No, some sins are unforgivable C. Yes, but only for believers D. No, forgiveness must be earned Answer: A How effective is the use of apocalyptic imagery in teaching moral lessons? A. It confuses young viewers B. It dramatizes the message of salvation C. It distracts from the core message D. It promotes fear Answer: B 🔹 Creating (Items 19–20) If you were to write a reflection based on this episode, what theme would you focus on? A. The importance of punishment B. The beauty of heaven C. The power of forgiveness D. The fear of judgment Answer: C What title would best capture the message of the episode? A. “The Fire Within” B. “Mercy Wins” C. “The End of Days” D. “Judgment and Justice” Answer: BMake a test, with answers best on the following: Conduct an investigation to provide evidence that living things are made of cells; either one cell or many different numbers and types of cells. Supporting Content LS1.A: Structure and Function • All living things are made up of cells, which is the smallest unit that can be said to be alive. An organism may consist of one single cell (unicellular) or many different numbers and types of cells (multicellular). (MS-LS-1.1) Further Explanation: Emphasis is on developing evidence that living things are made of cells, distinguishing between living and non-living things, and understanding that living things may be made of one cell or many and varied cells. In multicellular organisms, the body is a system of multiple interacting subsystems. These subsystems are groups of cells that work together to form tissues and organs that are specialized for particular body functions. (MS-LS-1.3) Further Explanation: Emphasis is on the conceptual understanding that cells form tissues and tissues form organs specialized for particular body functions. Examples could include the interaction of subsystems within a system and the normal functioning of those systems. Organisms reproduce, either sexually or asexually, and transfer their genetic information to their offspring. (MS-LS-1.4) • Living things share certain characteristics. (These include response to environment, reproduction, energy use, growth and development, life cycles, made of cells, etc.) (MS-LS1.4) Further Explanation: Examples should include both biotic and abiotic items, and should be defended using accepted characteristics of life. Plants, algae (including phytoplankton), and many microorganisms use the energy from light to make sugars (food) from carbon dioxide from the atmosphere and water through the process of photosynthesis, which also releases oxygen. These sugars can be used immediately or stored for growth or later use. (MS-LS-1.5) Further Explanation: Emphasis is on tracing movement of matter and flow of energy. Supporting Content LS1.C: Organization for Matter and Energy Flow in Organisms • Within individual organisms, food moves through a series of chemical reactions (cellular respiration) in which it is broken down and rearranged to form new molecules, to support growth, or to release energy. (MS-LS-1.6) Further Explanation: Emphasis is on describing that molecules are broken apart and put back together and that in this process, energy is released and on understanding that the elements in the products are the same as the elements in the reactants. Organisms, and populations of organisms, are dependent on their environmental interactions both with other living things and with nonliving factors. (MS-LS-2.1) • In any ecosystem, organisms and populations with similar requirements for food, water, oxygen, or other resources may compete with each other for limited resources, access to which consequently constrains their growth and reproduction. (MS-LS-2.1) • Growth of organisms and population increases are limited by access to resources. (MS-LS-2.1) Further Explanation: Emphasis is on cause and effect relationships between resources and growth of individual organisms and the numbers of organisms in ecosystems during periods of abundant and scarce resources. Similarly, predatory interactions may reduce the number of organisms or eliminate whole populations of organisms. Mutually beneficial interactions, in contrast, may become so interdependent that each organism requires the other for survival. Although the species involved in these competitive, predatory, and mutually beneficial interactions vary across ecosystems, the patterns of interactions of organisms with their environments, both living and nonliving, are shared. (MS-LS-2.2) Further Explanation: Emphasis is on predicting consistent patterns of interactions in different ecosystems in terms of the relationships among and between organisms and abiotic components of ecosystems. Examples of types of interactions could include competitive, predatory, and mutually beneficial. Food webs are models that demonstrate how matter and energy is transferred between producers, consumers, and decomposers as the three groups interact within an ecosystem. Transfers of matter into and out of the physical environment occur at every level. Decomposers recycle nutrients from dead plant or animal matter back to the soil in terrestrial environments or to the water in aquatic environments. The atoms that make up the organisms in an ecosystem are cycled repeatedly between the living and nonliving parts of the ecosystem. (MS-LS-2.3) Further Explanation: Emphasis is on describing the conservation of matter and flow of energy into and out of various ecosystems, and on defining the boundaries of the system. Ecosystems are dynamic in nature; their characteristics can vary over time. Disruptions to any physical or biological component of an ecosystem can lead to shifts in all its populations. (MSLS-2.5) Further Explanation: Emphasis is on recognizing patterns in data and making warranted inferences about changes in populations, and on evaluating empirical evidence supporting arguments about changes to ecosystems. Biodiversity describes the variety of species found in Earth’s terrestrial and oceanic ecosystems. The completeness or integrity of an ecosystem’s biodiversity is often used as a measure of its health. (MS-LS-2.6) Supporting Content LS4.D: Biodiversity • Changes in biodiversity can influence humans’ resources, such as food, energy, and medicines, as well as ecosystem services that humans rely on—for example, water purification and recycling. (MS-LS-2.6) Supporting Content ETS1.B: Developing Possible Solutions • There are systematic processes for evaluating solutions with respect to how well they meet the criteria and constraints of a problem. (MS-LS-2.6) Further Explanation: Examples of ecosystem services could include water purification, nutrient recycling, and prevention of soil erosion. Examples of design solution constraints could include scientific, economic, and social considerations. Genes are located in the chromosomes of cells, with each chromosome pair containing two variants of each of many distinct genes. Each distinct gene chiefly controls the production of specific proteins, which in turn affects the traits of the individual. Structural changes to genes (mutations) can result in changes to proteins, which can affect the structures and functions of the organism and thereby change traits. (MS-LS-3.1) Supporting Content LS3.B: Variation of Traits • In addition to variations that arise from sexual reproduction, genetic information can be altered because of mutations. Though rare, mutations may result in significant changes to the structure and function of proteins. Changes can be beneficial, harmful, or neutral to the organism. (MS-LS-3.1) Further Explanation: Emphasis is on conceptual understanding that changes in genetic material may result in making different proteins. Organisms reproduce, either sexually or asexually, and transfer their genetic information to their offspring. (MS-LS-3.2) Supporting Content LS3.A: Inheritance of Traits • Variations of inherited traits between parent and offspring arise from genetic differences that result from the subset of chromosomes (and therefore genes) inherited. (MS-LS-3.2) Supporting Content LS3.B: Variation of Traits • In sexually reproducing organisms, each parent contributes half of the genes acquired (at random) by the offspring. Individuals have two of each chromosome and hence two alleles of each gene, one acquired from each parent. These versions may be identical or may differ from each other. (MS-LS-3.2) Further Explanation: Emphasis is on using models such as simple Punnett squares and pedigrees, diagrams, and simulations to describe the cause and effect relationship of gene transmission from parent(s) to offspring and resulting genetic variation. The collection of fossils and their placement in chronological order is known as the fossil record and documents the change of many life forms throughout the history of the Earth. Anatomical similarities and differences between various organisms living today and between living and once living organisms in the fossil record enable the classification of living things. (MS-LS-4.1, MS-LS-4.2) Further Explanation: Emphasis is on finding patterns of changes in the level of complexity of anatomical structures in organisms and the chronological order of fossil appearance in the rock layers. The collection of fossils and their placement in chronological order is known as the fossil record and documents the change of many life forms throughout the history of the Earth. Anatomical similarities and differences between various organisms living today and between living and once living organisms in the fossil record enable the classification of living things. (MS-LS-4.1, MS-LS-4.2) Further Explanation: Emphasis is on explanations of the relationships among organisms in terms of similarity or differences of the gross appearance of anatomical structures. Scientific genus and species level names indicate a degree of relationship. (MS-LS-4.3) Further Explanation: Emphasis is on inferring general patterns of relatedness among structures of different organisms by comparing diagrams, pictures, specimens, or fossils. Natural selection leads to the predominance of certain traits in a population, and the suppression of others. (MS-LS-4.4) Further Explanation: Emphasis is on using concepts of natural selection, including overproduction of offspring, passage of time, variation in a population, selection of favorable traits, and heritability of traits. In artificial selection, humans have the capacity to influence certain characteristics of organisms by selective breeding. One can choose desired parental traits determined by genes, which are then passed to offspring. (MS-LS-4.5) Further Explanation: Emphasis is on identifying and communicating information from reliable sources about the influence of humans on genetic outcomes in artificial selection (such as genetic modification, animal husbandry, gene therapy), and on the influence these technologies have on society as well as the technologies leading to these scientific discoveries. Adaptation by natural selection acting over generations is one important process by which species change over time in response to changes in environmental conditions. Traits that support successful survival and reproduction in the new environment become more common; those that do not become less common. Thus, the distribution of traits in a population changes. (MS-LS-4.6) Further Explanation: Emphasis is on using mathematical models, probability statements, and proportional reasoning to support explanations of trends in changes to populations over time. Examples could include Peppered Moth population changes before and after the industrial revolution.