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Lide 1: Introduction to Bioreactor A bioreactor is a vessel used for growing microorganisms, plant or animal cells Provides controlled conditions for biological reactions Maintains optimum pH, temperature, oxygen, and nutrients Widely used in fermentation, enzyme, vaccine, and antibiotic production Ensures sterile and aseptic environment Scale ranges from laboratory to industrial production Slide 2: Basic Design Requirements of a Bioreactor Must be constructed with non-toxic, corrosion-resistant materials Should allow effective mixing and mass transfer Provision for sterilization (in situ sterilization) Must maintain uniform temperature and pH Easy sampling without contamination Should support scalability and automation Slide 3: Materials Used in Bioreactor Construction Stainless steel (SS-316) for industrial bioreactors Glass for laboratory-scale bioreactors Plastic (polycarbonate) for disposable bioreactors Materials must withstand heat and pressure Should be smooth to prevent microbial attachment Resistant to chemicals and cleaning agents Slide 4: Main Parts of a Bioreactor Vessel: holds the culture medium and microorganisms Agitator (impeller): provides mixing Sparger: supplies sterile air Baffles: prevent vortex formation Sensors: monitor pH, temperature, dissolved oxygen Ports: used for inoculation, sampling, and feeding Slide 5: Agitation System Ensures uniform mixing of nutrients and cells Improves oxygen transfer rate Common impellers: Rushton turbine, marine propeller Speed controlled by motor Prevents settling of cells Affects shear stress on cells Slide 6: Aeration System Supplies oxygen for aerobic fermentation Air introduced through sparger Types of spargers: ring, nozzle, sintered Maintains dissolved oxygen concentration Air is filtered for sterility Essential for high cell density cultures Slide 7: Temperature and pH Control Temperature controlled by heating/cooling jackets pH maintained using acid or alkali addition Sensors continuously monitor parameters Automated control systems used Ensures optimal microbial growth Prevents enzyme denaturation Slide 8: Foam Control System Foam formed due to protein and agitation Excess foam reduces oxygen transfer Mechanical foam breakers used Chemical antifoam agents added Foam sensor detects foam formation Maintains efficient fermentation Slide 9: Types of Bioreactors – Based on Mode of Operation Batch bioreactor Fed-batch bioreactor Continuous bioreactor Choice depends on product type Widely used in industrial fermentation Controls productivity and yield Slide 10: Batch Bioreactor All nutrients added at the beginning No addition or removal during process Simple and easy to operate Low risk of contamination Used for antibiotics and enzymes Limited control over nutrient depletion Slide 11: Fed-Batch Bioreactor Nutrients added during fermentation Prevents substrate inhibition High product yield Widely used in industrial fermentation Allows better control of growth rate Used in insulin and enzyme production Slide 12: Continuous Bioreactor Fresh medium continuously added Culture removed at same rate Maintains steady-state conditions High productivity Risk of contamination is high Used in wastewater treatment and SCP production Slide 13: Types of Bioreactors – Based on Design Stirred tank bioreactor Airlift bioreactor Bubble column bioreactor Packed bed bioreactor Fluidized bed bioreactor Photobioreactor Slide 14: Stirred Tank Bioreactor (STR) Most commonly used bioreactor Mechanical agitation using impellers Suitable for aerobic fermentation Excellent mixing and oxygen transfer Used for bacteria and fungi Easy scale-up Slide 15: Airlift Bioreactor Mixing achieved by air circulation No mechanical agitator Low shear stress Energy efficient Suitable for shear-sensitive cells Used in wastewater treatment Slide 16: Bubble Column Bioreactor Air bubbles provide mixing Simple design and low cost No moving parts Limited mixing efficiency Used for microbial fermentation Suitable for large-scale operations Slide 17: Packed Bed Bioreactor Contains immobilized cells or enzymes Substrate flows through packed matrix High cell density Used in continuous processes Limited oxygen transfer Used in enzyme and wastewater treatment Slide 18: Fluidized Bed Bioreactor Immobilized particles kept in suspension Better mass transfer than packed bed Reduced clogging Suitable for continuous operation Used in biotransformations Higher operational complexity Slide 19: Photobioreactor Designed for photosynthetic organisms Provides light source Used for algae and cyanobacteria Controls light, CO₂, and temperature Used in biofuel and pigment production Can be tubular or flat-plate design Slide 20: Applications of Bioreactors Production of antibiotics and vaccines Enzyme and organic acid production Single cell protein production Wastewater treatment Biofertilizer and biopesticide production Biopharmaceutical manufacturing

Use the questions and answers below to make a 10 question quiz: Which principle of interactive media is most impacted by its ‘ubiquity’? A) Restricted access control B) Global market presence and integration C) Limited reach to specific user demographics D) Single-channel broadcasting Answer: B Which feature of interactive media ensures that users can actively control and manipulate the content they access? A) Multimedia integration B) UI simplification C) User interactivity D) System automation Answer: C How does globalisation enhance user experience in interactive media systems, according to 1.1.1? A) By reducing content to a single cultural standard B) By supporting diverse user needs through varied, accessible content C) By enforcing a common global pricing structure D) By allowing one-way communication only Answer: B Why is copyright compliance critical in the context of interactive media? A) It allows users to freely distribute content B) It supports ethical use and protects creators from unauthorized distribution C) It restricts all users from accessing online media D) It focuses solely on preventing duplication of digital games Answer: B In interactive media, 'UX' primarily focuses on what aspect of user engagement? A) Monitoring users’ online activities B) Enabling dynamic user experiences tailored to user intent and satisfaction C) Simplifying multimedia formats to save storage D) Reducing user interaction to maintain control Answer: B What is a key distinction between ‘social issues’ and ‘ethical issues’ in interactive media development? A) Social issues focus on technical concerns, while ethical issues are user-centered B) Social issues relate to user interactions, while ethical issues focus on moral responsibilities C) Social issues address individual rights, whereas ethical issues involve systemic improvements D) Social issues are about compliance, whereas ethical issues concern legal standards Answer: B Which component is crucial to creating an accessible interactive media system for users with disabilities? A) High-end processing units B) Customizable UI elements, such as voice and visual aids C) Limited content based on user demographics D) Exclusive copyright protections Answer: B How does data quality contribute to an interactive media system's success? A) By maximizing data storage for multimedia content B) By ensuring content is relevant, current, and accurate for the intended audience C) By focusing on visual appeal over functionality D) By reducing interaction requirements to enhance performance Answer: B Which factor in interactive media systems enhances global engagement through a seamless user experience? A) Interactivity B) Ubiquity C) Restricted Access D) Content Redundancy Answer: B Why might legal implications arise in interactive media systems despite ethical intentions? A) Compliance with global standards B) Misinterpretations of user intent in a diverse cultural context C) Excessive content validation D) Limited user feedback channels Answer: B

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Make a multiple choice test with the follwo1. Causation 13. Abetting 25. Restitution 2. Direct Evidence 14. Private Law 26. Writ of Summons 3. Automatism 15. Prejudice 27. Actus Reus 4. Self-Defense 16. Contributory Negligence 28. Net Family Property 5. Reasonable Limits Clause 17. Duress 29. Poisoned Environment 6. Standard of Care 18. Circumstantial Evidence 30. Mediation 7. Habeus Corpus 19.. Public Law 31. Act 8. Oakes Test 20. Accessory After the Fact 32. Prima Facie 9. Pecuniary 21. Hybrid Offence 33. Opinion Evidence 10. Mens Rea 22. Aiding 34. Indictable Offence 11. Marriage Breakdown 23. Manslaughter 35.Unintentional Discrimination 12. Crown 24. Empanellinging terms:

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