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Ch3 Part 1 Eukaryotic cell structure and cell fractionation
Quiz by Mr R Horn
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What scale is used to measure the concentration of H+ ions in a solution (acidity/alkalinity)
Before beginning the process of cell fractionation the tissue is placed in a buffered solution. Explain why.
Before beginning the process of cell fractionation the tissue is placed in an ice cold solution. Explain why.
Before beginning the process of cell fractionation the tissue is placed in an isotonic solution. Explain why.
Name the first stage of cell fractionation, in which organelles are released from cells (by breaking them open using a blender)
During cell fractionation, after homogenation the resulting fluid (homogenate) must be filtered. Why?
Identify which organelles are found in the 1st, 2nd, 3rd, 4th and 5th pellets
What term is used to refer to the sediment that forms at the bottom of a centrifuge tube following centrifugation?
What term is used to refer to the fluid at the top of a centrifuge tube following centrifugation?
True or false? Once the 1st pellet has formed the supernatant is poured into another centrifuge tube and spun at a higher speed to form the second pellet.
Name the foldings of the inner mitochondrial membrane
Name the region in the centre of mitochondria, containing enzymes, DNA and ribosomes