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Correct the mistakes pres perfect and other
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Correct the mistakesObjective 2 Correct the mistakes Revision 3: Correct the mistakesResearch: Scientific Attitudes These are the traits that scientists and researchers practice to ensure reliable results and good research work: Curiosity – Desire to ask questions and seek answers. Drives exploration and discovery. Example: Wondering why leaves change color in autumn. Intellectual Honesty – Reporting observations and results truthfully, even if they don’t support your hypothesis. Open-Mindedness – Willingness to accept new ideas and consider other perspectives. Perseverance – Continuing research despite difficulties or failures. Objectivity – Avoiding bias; basing conclusions only on evidence and facts. Positive Attitude Towards Failure – Viewing mistakes as opportunities to learn and improve. Skepticism – Questioning results and not accepting claims without sufficient evidence. Observation and Inference Observation – Using the senses (or tools) to gather information. Qualitative Observation → Describes qualities (color, shape, texture). Quantitative Observation → Uses numbers or measurements (height, mass, temperature). Inference – Logical explanation or conclusion based on observations and prior knowledge. Example: Seeing smoke and inferring there is fire. 🔎 Science Process Skills These are steps used in scientific investigations: Observing – Using senses and instruments to gather data. Inferring – Making explanations based on observations. Predicting – Stating what you think will happen based on patterns or evidence. Communicating – Sharing results through words, graphs, charts, or reports. Classifying – Grouping objects or data according to similarities and differences. Ordering/Sequencing – Arranging objects or events in correct order (time, size, importance). Measuring – Using standard units and instruments to describe length, mass, volume, time, etc. 📏 Measurement and Measuring Instruments Measurement – The process of comparing an unknown quantity with a standard unit. Common Quantities and Instruments: Length/Distance – Ruler, Meter Stick, Vernier Caliper, Tape Measure. Mass – Balance (triple beam, electronic). Volume – Graduated Cylinder, Measuring Cup, Pipette, Burette. Temperature – Thermometer. Time – Stopwatch, Clock. Electric Current – Ammeter. Voltage – Voltmeter. Key Idea: Accurate measurement requires using the correct instrument and unit (SI Units).DNA polymerase III A prokaryotic enzyme with 5’ to 3’ Polymerase activity. The Hydrolysis of pyrophosphate
(PPi) drives the energy required to create the covalent
phosphodiester bond.A prokaryotic enzyme with 5’ to 3’ Polymerase activity.DNA polymerase is a multisubunit enzyme where the subunits of the enzyme form a ring that encircles and
moves along the template strand of the DNA, thus serving
as a sliding DNA clamp. Therefore the enzyme cannot
diffuse away from its task.DNA polymerase III has proofreading capabilities and therefore the error rate of RNA transcription
is 1 in 10 million bases (1X107).To ensure DNA replication fidelity, DNA polymerase III can edit its mistakes. DNA polymerase III has 3’ to 5’ Exonuclease activity.The 5’ to 3’ polymerase
activity then replaces it with the correct nucleotide.Kindly create a 30 items multiple choice test from this laboratory activity entitled laboratory do's and donts: LABORATORY SAFETY Dos: Wear Appropriate Attire: Wear lab coats, safety goggles, gloves, and any other required personal protective equipment (PPE) at all times in the lab. Follow Protocols: Adhere strictly to established protocols and procedures for all experiments and tasks. Label Everything: Clearly label all containers, tubes, vials, and equipment with relevant information, including date, contents, and your initials. Calibrate Instruments: Regularly calibrate and maintain all lab equipment according to manufacturer guidelines to ensure accurate measurements. Keep Workspace Organized: Maintain a clean and organized workspace to prevent contamination and ensure efficient work. Dispose of Waste Properly: Follow the correct disposal procedures for hazardous waste, sharps, and non-hazardous materials in accordance with local regulations. Use Pipette Aids: Always use pipette aids or bulb fillers to avoid mouth pipetting and potential exposure to hazardous substances. Record Observations: Keep detailed and accurate records of your experiments, observations, procedures, and results. Label Samples Clearly: Label all samples with accurate and descriptive information to avoid mix-ups and confusion. Communicate: Maintain clear communication with colleagues and supervisors about your work, findings, and any potential issues. Follow Safety Guidelines: Adhere to all safety guidelines, emergency procedures, and evacuation plans in case of accidents or incidents. Report Accidents and Incidents: Report any accidents, spills, or incidents to your supervisor immediately, no matter how minor they may seem. Don'ts: Don't Eat, Drink, or Smoke: Never consume food, drinks, or smoke inside the laboratory to prevent contamination and chemical exposure. Don't Pipette by Mouth: Avoid mouth pipetting to prevent the risk of inhaling or ingesting hazardous substances. Don't Use Chipped Glassware: Do not use chipped, cracked, or compromised glassware, as they can lead to leaks and contamination. Don't Work Alone: Avoid working in the lab alone, especially with hazardous materials or equipment. Don't Ignore Safety Procedures: Never disregard safety procedures or skip steps, even if you're experienced with a particular task. Don't Contaminate Reagents: Avoid contaminating reagents by using clean tools, pipettes, and containers. Don't Rush: Take your time and follow protocols accurately. Rushing can lead to mistakes and unsafe conditions. Don't Block Emergency Equipment: Keep emergency equipment, such as eyewash stations, fire extinguishers, and safety showers, unobstructed and easily accessible. Don't Pour Chemicals into Sinks: Do not pour chemicals down sinks unless you are certain they are safe to do so, as this can lead to environmental contamination. Don't Use Unlabeled Chemicals: Never use unlabeled or improperly labeled chemicals. Always know what you're working with. Don't Wear Loose Clothing or Jewelry: Avoid wearing loose clothing, open-toed shoes, and excessive jewelry that could get caught in equipment or chemicals. Don't Assume, Ask: If you're unsure about something, never assume. Always ask for guidance from your supervisor or colleagueHow is personal data collected? There are several ways that an unauthorised person can try and collect your data. These include: •phishing •smishing •vishing •pharming. Phishing Phishing is when a person sends a legitimate looking email to a user. The email contains a link to a website that also looks legitimate. The user is encouraged to click the link and to input personal data into a form on the website. The email could also simply ask the user to reply to the email with their personal data. The user is tricked into giving their personal data to a source that they believe is legitimate. However, both the email and the linked website are from a fake unauthorised source. The personal data that is input is then collected by an unauthorised person. This person can then use this data for criminal acts, for example, to commit fraud or steal the person's identity. Intimidation has become a common feature of phishing emails, threatening the user that they must click the link and rectify a situation immediately, or there will be a further issue. The aim of a phishing attack is to steal the user's personal data. Figure 5.1: Phishing. A real-life example of phishing PayPal have been the subject of several different phishing emails. Users receive an email that looks as though it has been sent from PayPal, as it has the PayPal branding. The email normally warns of an issue such as unexpected activity on their account, or that some kind of verification of their account is required. The user is then asked to click a link to log into their account and resolve the issue. The link takes them to a webpage that looks like the PayPal login page. If the user inputs their login details into this page, they will not be taken to their account. It is often at this stage that the user may realise that the email and webpage are fake. However, they have already given the unauthorised person their PayPal login details. Figure 5.2: An example of a phishing email claiming to be from PayPal. How to recognise phishing There are several guidelines to be aware of regarding emails to avoid being subjected to phishing. These include: •Don't even open an email that is not from a sender that you recognise or a trusted source. •Legitimate companies will never ask you for your personal data using email. Be immediately suspicious of any email that requests your personal data. •Legitimate companies will normally address you by your name. Be suspicious of any email that addresses you as ‘Dear Member' or ‘Dear Customer'. •Legitimate companies will send an email that uses their domain name. If you hover your mouse over the sender's name, it will show the email address that the email is sent from. If this does not look legitimate, for example, does not contain the correct domain name, then it is probably fake. For example, if the sender's email is user@paypal1.com rather than user@paypal.com, this is from an incorrect domain name. •Legitimate companies are protective of their professional reputation and thoroughly check any communications. They will make sure that all information given is grammatically and correctly spelt. Be suspicious of any email that contains bad grammar or spelling mistakes. •A link in an email from a legitimate company will also normally contain the domain name of the company. You can sometimes hover over the link, or right click and inspect the link, to see the address of the URL that is attached. If the URL does not contain the domain name, or also contains typical errors such as spelling mistakes, then be suspicious of this. PRACTICAL ACTIVITY 5.02 Ask a friend or a member of your family if they have ever received an email that they believed was a phishing email. Ask them how they identified it was phishing. Ask them if they know all of the given guidelines for identifying phishing emails. Smishing Smishing (or SMS phishing) is a variant of phishing that uses SMS text messages to lure the user into providing their personal details. The user is sent an SMS text message that either contains a link to a website, in the same way that phishing does, or it will ask the user to call a telephone number to resolve an urgent issue. The same advice can be followed for smishing as given for phishing. The user must question at all times any links that are sent from an unknown or suspicious user. It is advisable that if a user believes the message may be legitimate, to type in the domain name for the legitimate company website into their web browser, rather than following the link in the message. Users should block any numbers that they believe are suspicious to prevent any further risk of smishing from that number. Figure 5.3: Smishing. Vishing Vishing (or voice phishing) has the same aim as phishing, to obtain a user's personal details. The user receives a telephone call that could either be an automated system or could be a real person. An automated voice could speak to the user and advise them that an issue has occurred, such as there has been suspicious activity regarding their bank account. The user may then be asked to call another number, or just to simply press a digit and be directed to another automated system. This system will ask them to provide their bank account details to resolve the issue. The bank account details have then been obtained by the unauthorised user and can be used to commit a crime against the user. The automated system could be replaced by a real person who will try to do the same thing. They will try to convince the user that there has been an issue with an account they have and to provide the log-in details or PIN for the account to verify who they are so the issue can be resolved. The precaution to take for vishing is that no company will ever call you and ask you to provide any log-in details or PIN details over the telephone. They may ask you to provide other personal information, and if you are in doubt that the person on the other end of the phone is legitimate, it is always advisable to put the phone down and call the company back on a legitimate number that you may already know or can obtain. Figure 5.4: Vishing. Pharming Pharming is when an unauthorised user installs malicious code on a person's hard drive or server. The malicious code is designed to redirect a user to a fake website when they type in the address of a legitimate one. The fake website is designed to look like the legitimate one, to trick the user and make sure they are not aware that their request has been redirected. The user will then enter their personal details into the fake website, believing it is the legitimate one, and the unauthorised person will now have their personal data. A common technique used in pharming is called domain name server (DNS) cache poisoning. This technique exploits vulnerabilities in the DNS and diverts the internet traffic intended for a legitimate server toward a fake one instead. The unauthorised user needs to find a way to install the malicious code on the computer. They often hide the malicious code in an email attachment or link. When the user opens the email attachment or clicks the link, the malicious code is downloaded also. Figure 5.5: Pharming. The aim of a pharming attack is also to steal a user's personal data. A real-life example of pharming In 2007 50 different companies all over the world were subject to a pharming attack, these included PayPal, eBay, Barclays bank and American Express. Over a three-day period, hackers managed to infect over 1000 PCs a day with a malicious pharming code. When users who had been infected visited the websites of the different companies, they were redirected to a legitimate-looking version of the site that was designed to steal their personal data. The original email, containing the malicious code, was set up to look like a shocking news story. Users were encouraged to click a link in the email to find out more information. The code was downloaded when the user clicked the link. This was quite a sophisticated attack that required legitimate looking websites to be set up for a large number of companies. It is not known how much money the hackers were able to retrieve as a result. How to prevent pharming All of the guidelines to avoid being subjected to phishing are also relevant for recognising pharming. There are also several other precautions that can be taken to check for pharming attacks. These include: •Have a firewall installed and operational. A firewall monitors incoming and outgoing traffic from your computer. It checks this traffic against set criteria and will flag and stop any traffic that does not meet the criteria. A firewall could detect and block suspicious traffic, such as a malicious code trying to enter your system. •Have an anti-virus program installed that is designed to detect malicious pharming code. You need to scan your computer on a regular basis to check for any malicious code. It is advisable to set up an automatic scan on a daily basis at a time when your computer will normally be switched on. •Be aware when using public Wi-Fi connections. A hacker could look to directly access your computer and install the malicious code if you are connected to a public Wi-Fi connection. It is often advisable to use a VPN when using public Wi-Fi. This will help shield your internet activity and personal details from a hacker, making it more difficult for them to access your computer. Smishing can also be used as a form of pharming. A user is sent a link, that when they click is designed to download malware onto their mobile device. Therefore, it is advisable to have security software installed on your mobile and also scan it regularly to detect any presence of malware.Question,Correct Answer,Option 2,Option 3,Option 4 You just saw your crush trip, but you imagine it in slow‑motion as if in a movie. What are you doing?,Visualizing,Ignoring,Running away,Screaming Your best friend says your outfit is “trash” but you think it’s ICONIC! This shows —,Perception,Precision,Symmetry,Association A butterfly’s wings are perfectly matching on both sides. They are —,Symmetrical,Random,Loud,Vague You always think of pizza when you hear “Friday night.” This is a(n) —,Association,Obsession,Illustration,Symmetry You carefully measure 10 candies for lunch. This shows —,Precision,Obsession,Symmetry,Association A teacher draws a big volcano on the board to explain it. She is —,Illustrating,Guessing,Visualizing,Ignoring Two students react differently to the same horror movie. This shows —,Perception,Symmetry,Precision,Obsession You measure your eyeliner perfectly on both eyes. This is —,Symmetry,Visualizing,Association,Individualized A student redoes ONE sentence 17 times all night. She is —,Obsessive,Relaxed,Careless,Confused A loud, clear knock on the door is —,Distinct,Vague,Random,Individualized You picture your future life as a superhero saving the world. You are —,Visualizing,Associating,Obsessing,Measuring A quiz made just for ONE student is —,Individualized,General,Random,Symmetrical Two students get the same task but react differently. This shows differences in —,Perception,Symmetry,Association,Obsession Your friend always folds napkins perfectly before dinner. That is —,Obsessive,Relaxed,Visualizing,Association A student triple‑checks a ruler, erases tiny mistakes, and won’t submit. Which TWO describe her? (Choose best single answer),Obsessive and Precise,Relaxed and Calm,Confused and Random,Symmetrical and Vague Which shows BOTH perception AND association?,Thinking rain is sad because you cried in it,Measuring carefully,Folding paper,Drawing a volcano The **funniest way to show symmetry** in class would be —,Balancing a pencil on your nose randomly,Throwing paper randomly,Screaming at the board,Balancing a pencil perfectly on your nose You hear a scary clear knock and spill your water. That knock is —,Distinct,Vague,Individualized,Symmetry Which is NOT individualized?,Standard school uniform,Custom shoes,Test for one student,Personalized notebook Two students measure carefully. One is calm and exact, the other keeps freaking out. The calm student shows —,Precision,Obsession,Association,Illustration The same scenario: The freaking‑out student shows —,Obsession,Symmetry,Visualizing,Precision