¿Cuál de las siguientes afirmaciones sobre CRISPR en bacterias es correcta?

CRISPR actúa como un sistema inmunológico adaptativo en bacterias

CRISPR es un tipo de proteína que destruye el ADN de las bacterias

CRISPR solo puede ser utilizado en organismos multicelulares

CRISPR es un tipo de virus que infecta bacterias

¿Cuál de las siguientes aplicaciones NO se asocia típicamente con el uso de CRISPR en bacterias?

Producción de proteínas humanas

Estudio de la función de genes específicos

Desarrollo de cepas bacterianas resistentes a antibióticos

¿Cuál es la función principal de la proteína Cas9 en el sistema CRISPR de las bacterias?

Cortar el ADN en sitios específicos

¿Cuál es una limitación del uso de CRISPR en bacterias?

Posibilidad de efectos fuera del objetivo (off-target)

Solo funciona en bacterias grampositivas

El tiempo de edición es demasiado largo

¿Qué rol juega el RNA de molécula CRISPR en el sistema de defensa bacteriano?

Sirve como guía para la proteína Cas9

Fomenta la replicación del ADN

¿Cuál es un uso potencial de CRISPR en el ámbito de la biotecnología?

Desarrollo de cultivos resistentes a enfermedades

Fusión de diferentes especies de plantas

Producción de materiales plásticos

¿Qué proporciona el sistema CRISPR a las bacterias que les permite recordar infecciones virales pasadas?

Secuencias de RNA de las infecciones anteriores

Memoria a largo plazo en el ADN

Anticuerpos producidos por la bacteria

Proteínas específicas para cada virus

¿Qué técnica se utiliza comúnmente junto con CRISPR para confirmar ediciones genéticas en bacterias?

Reacción en cadena de la polimerasa (PCR)

Análisis de microscopía electrónica

CRISPR en Bacterias

CRISPR in Bacteria

CRISPR bei Bakterien

CRISPR chez les bactéries

Advantages and disadvantages of CRISPR technique

Which cellular mechanism is utilized by CRISPR-Cas9 to perform a gene knockout?

Allele variation of a specific gene Artificial Insemination (AI) collecting and preserving semen from sires and using artificial means to introduce it to the dam’s reproductive tract Body Cells make up the organs and tissue of an animal and have chromosomes in pairs, called diploids Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene editing technology Codominance heterozygous individual expresses the phenotype of both alleles simultaneously Complete Dominance heterozygous gene pair is expressed the same as a homozygous dominant gene pair Crossbreeding sire from one breed and a dam from another, with each breed excelling in a certain characteristic to benefit the producer and the offspring Deoxyribonucleic Acid (DNA) stores genetic information and acts as a blueprint for all genetic material in the organism in two strands arranged in a double helix Dominant Alleles represent a dominant phenotype and are expressed as uppercase letters Embryo Transfer eggs are collected from a desirable female, fertilized and then implanted in several other females Expected Progeny Differences (EPDs) measure of the heritability of breeding values and traits Gametes fulfill the purpose of sexual reproduction, passing on half of the genetic code in the form of sperm and ovum and are also called haploids or sex cells Genotype organism's genetic composition, which determines its heredity potential and limitations Grading Up using a purebred sire to breed grade (unregistered or commercial) females Heritability degree to which offspring resemble their parent for a particular trait Heterosis (Hybrid Vigor) ability of crossbred animals to have the best traits from each parent Heterozygosity phenomenon of inheriting a different version of an allele from each biological parent Homozygosity phenomenon of inheriting the same version of an allele from each biological parent Inbreeding breeding of closely related animals with the goal of concentrating traits from a superior individual Incomplete Dominance dominant allele does not completely overcome the recessive Law of Dominance states genes will express themselves with the dominant gene appearing in the phenotype Law of Independent Assortment states unlinked or distantly linked gene pairs separate independently of other genes Law of Segregation states paired genes must segregate equally into gametes in a way so offspring have an equal likelihood of inheriting either factor Locus place on a chromosome where a gene is found Meiosis process of cellular reproduction of gametes and results in four genetically different daughter cells Mitosis process of cellular reproduction of body cells which creates two genetically identical daughter cells Outcrossing breeding highly unrelated individuals within a breed Phenotype all the observable characteristics of an organism resulting from the interaction of its genotype with its environment Polygenic Traits controlled by many gene pairs Punnett Square graphical representation of the possible genotypes of an offspring arising from a particular breeding, using letters to represent the genes Recessive Alleles non-dominant phenotypes which can still affect the appearance of an animal, but not as commonly, and are expressed as lowercase letters Ribonucleic Acid (RNA) replicates genetic information found in DNA to build proteins in processes known as transcription and translation Simply Inherited Traits typically controlled by one gene pair

crisps - work p. 42

Introducción a CRISP-DM

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