Question 1

During DNA replication, which enzyme is responsible for sealing the gaps between Okazaki fragments?

Accepted Answer

DNA ligase

Answer

DNA helicase

Answer

Primase

Answer

DNA polymerase

Question 2

What direction does DNA polymerase synthesize new DNA strands during replication?

Accepted Answer

5' to 3'

Answer

3' to 5'

Answer

Random direction

Answer

Left to right

Question 3

During DNA replication, which enzyme is primarily responsible for unwinding the DNA strands?

Accepted Answer

Helicase

Answer

Primase

Answer

Ligase

Answer

Polymerase

Question 4

What is the significance of RNA primers in DNA replication?

Accepted Answer

They provide a starting point for DNA synthesis

Answer

They unwind the DNA strands

Answer

They degrade the old DNA strands

Answer

They seal the gaps between Okazaki fragments

Question 5

What is the purpose of the leading strand during DNA replication?

Accepted Answer

To be synthesized continuously

Answer

To unwind the DNA strands

Answer

To be synthesized in fragments

Answer

To act as a template for RNA synthesis

Question 6

Which of the following proteins is responsible for sealing nicks in the DNA backbone during replication?

Accepted Answer

DNA ligase

Answer

DNA helicase

Answer

Topoisomerase

Answer

DNA primase

Question 7

What role does topoisomerase play during DNA replication?

Accepted Answer

Relieving torsional strain ahead of the replication fork

Answer

Sealing nicks in the DNA strands

Answer

Slicing open the DNA strands

Answer

Synthesizing RNA primers

Question 8

What determines the sequence of nucleotides in a newly synthesized DNA strand during replication?

Accepted Answer

The template strand of DNA

Answer

The Okazaki fragments

Answer

The enzyme DNA polymerase

Answer

The RNA primers

Question 9

What effect does the presence of telomeres have on DNA replication?

Accepted Answer

They protect chromosome ends from degradation

Answer

They enable replication to occur continuously

Answer

They facilitate the binding of DNA polymerase

Answer

They increase the speed of replication

Question 10

Which protein prevents torsion of the double helix by "nicking" the backbone of DNA?

Accepted Answer

Topoisomerase

Answer

Helicase

Answer

Single stranded binding protein

Answer

Ligase

Question 11

During DNA replication, which strand is synthesized continuously?

Accepted Answer

Leading strand

Answer

Okazaki strand

Answer

Lagging strand

Answer

Discontinuous strand

Question 12

Which is responsible for removing primers

Accepted Answer

DNA polymerase I

Answer

Ligase

Answer

DNA polymerase III

Answer

Primase

Question 13

Which of the following replaces RNA nucleotides with DNA nucleotides?

Accepted Answer

DNA polymerase I (U to T)

Answer

DNA polymerase III (T to U)

Answer

DNA polymerase I (T to U)

Answer

DNA polymerase III (U to T)

Question 14

_____ reads the template and synthesizes a new DNA strand

Accepted Answer

DNA polymerase III

Answer

DNA polymerase I

Answer

Topoisomerase

Answer

Primase

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