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DNA Transcription and DNA Translation
Quiz by Kaleb Hudlow
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SB2. Obtain, evaluate, and communicate information to analyze how genetic information is expressed in cells. a. Construct an explanation of how the structures of DNA and RNA lead to the expression of information within the cell via the processes of replication, transcription, and translation. Learning Targets _______Identify the structural components of DNA and RNA Success Criteria _______Can accurately identify the key structural components of DNA (deoxyribose sugar, phosphate group, nitrogenous bases: adenine, thymine, cytosine, guanine). _______Can identify the key structural components of RNA (ribose sugar, phosphate group, nitrogenous bases: adenine, uracil, cytosine, guanine). _______Can describe the differences in the sugar backbone of DNA and RNA (deoxyribose vs. ribose). _______Can identify the double-stranded structure of DNA and the single-stranded structure of RNA. _______Identify the parts of protein synthesis and the location of each process Success Criteria _______Can identify and describe the two main processes of protein synthesis: transcription and translation. _______Can correctly explain that transcription occurs in the nucleus where DNA is transcribed into mRNA. _______Can explain that translation occurs in the cytoplasm at ribosomes where mRNA is translated into amino acid sequences to form proteins. _______Compare and Contrast DNA to RNA Success Criteria _______Can clearly identify similarities between DNA and RNA, such as both being nucleic acids and containing nucleotide structures. _______Can explain differences in DNA and RNA, including sugar types (deoxyribose vs. ribose), strand number (double-stranded DNA vs. single-stranded RNA), and nitrogenous base usage (thymine in DNA vs. uracil in RNA). _______Can describe the function of DNA as genetic storage and the function of RNA in protein synthesis (mRNA, tRNA, rRNA). _______Analyze the reasoning for enzymes usage in both DNA replication and protein synthesis. Success Criteria _______Can identify key enzymes involved in DNA replication (e.g., helicase, DNA polymerase, ligase) and explain their functions (e.g., helicase-unwinding DNA, DNA polymerase-synthesizing new DNA strands, ligase-sealing nicks in the DNA backbone). _______Can identify enzymes involved in protein synthesis (e.g., RNA polymerase) and explain their role in transcribing DNA into mRNA. _______Can analyze why enzymes are essential for speeding up chemical reactions…(ensuring accuracy, and catalyzing steps in replication and protein synthesis) _______Can provide specific examples of how enzyme malfunction can impact genetic replication or protein synthesis. _______Perform the steps of DNA replication and protein synthesis in order to demonstrate their understanding of how the structure of DNA supports the genetic expression in successive generations. Success Criteria _______Can demonstrate a step-by-step understanding of DNA replication, including unwinding, complementary base pairing, and proofreading. _______Can demonstrate the steps of transcription (formation of mRNA from DNA) and translation (conversion of mRNA into a polypeptide). _______Can show how DNA's structure (double helix, base pairing) ensures accurate replication for passing genetic information to offspring. _______Can illustrate how changes in DNA sequence can lead to changes in protein structure and function, thus affecting traits in successive generations.B.6C Transcription and Translation for DNA/RNARNA translation. The genetic information in DNA is used to synthesize proteins through the processes of RNA Transcription and RNA Translation. The sequence of three-letter code words in the gene determines the sequence of amino acids in a polypeptide chain. RNA Transcription DNA serves as a template for the synthesis of RNA. There are 3 major types of RNA that participate in the process of proteins synthesis. 1.5% of the genome encodes for proteins via mRNA, but an unknown % of the genome codes for noncoding RNA (ncRNA)History and structure of DNA, DNA Replication, Transcription and translation. What is DNA? What are genes? DNA structure.Allele variation of a specific gene Artificial Insemination (AI) collecting and preserving semen from sires and using artificial means to introduce it to the dam’s reproductive tract Body Cells make up the organs and tissue of an animal and have chromosomes in pairs, called diploids Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) gene editing technology Codominance heterozygous individual expresses the phenotype of both alleles simultaneously Complete Dominance heterozygous gene pair is expressed the same as a homozygous dominant gene pair Crossbreeding sire from one breed and a dam from another, with each breed excelling in a certain characteristic to benefit the producer and the offspring Deoxyribonucleic Acid (DNA) stores genetic information and acts as a blueprint for all genetic material in the organism in two strands arranged in a double helix Dominant Alleles represent a dominant phenotype and are expressed as uppercase letters Embryo Transfer eggs are collected from a desirable female, fertilized and then implanted in several other females Expected Progeny Differences (EPDs) measure of the heritability of breeding values and traits Gametes fulfill the purpose of sexual reproduction, passing on half of the genetic code in the form of sperm and ovum and are also called haploids or sex cells Genotype organism's genetic composition, which determines its heredity potential and limitations Grading Up using a purebred sire to breed grade (unregistered or commercial) females Heritability degree to which offspring resemble their parent for a particular trait Heterosis (Hybrid Vigor) ability of crossbred animals to have the best traits from each parent Heterozygosity phenomenon of inheriting a different version of an allele from each biological parent Homozygosity phenomenon of inheriting the same version of an allele from each biological parent Inbreeding breeding of closely related animals with the goal of concentrating traits from a superior individual Incomplete Dominance dominant allele does not completely overcome the recessive Law of Dominance states genes will express themselves with the dominant gene appearing in the phenotype Law of Independent Assortment states unlinked or distantly linked gene pairs separate independently of other genes Law of Segregation states paired genes must segregate equally into gametes in a way so offspring have an equal likelihood of inheriting either factor Locus place on a chromosome where a gene is found Meiosis process of cellular reproduction of gametes and results in four genetically different daughter cells Mitosis process of cellular reproduction of body cells which creates two genetically identical daughter cells Outcrossing breeding highly unrelated individuals within a breed Phenotype all the observable characteristics of an organism resulting from the interaction of its genotype with its environment Polygenic Traits controlled by many gene pairs Punnett Square graphical representation of the possible genotypes of an offspring arising from a particular breeding, using letters to represent the genes Recessive Alleles non-dominant phenotypes which can still affect the appearance of an animal, but not as commonly, and are expressed as lowercase letters Ribonucleic Acid (RNA) replicates genetic information found in DNA to build proteins in processes known as transcription and translation Simply Inherited Traits typically controlled by one gene pairDNA replication and transcriptionThe Promoter Region is a region of DNA upstream where transcription is initiated for control of gene transcription Promoters are solely important for gene expression Pribnow Box in Prokaryotes. CAAT and Hogness box (TATA) in Eukaryotes They control the binding of RNA polymerases and Transcription factors (if Eukaryotic) Now RNA polymerase can transcribe DNA to RNA to further achieve the goal to mRNA to eventual protein formation Prokaryotic promoter are recognized by RNA polymerase and sigma factor (Sigma Subunit) Eukaryotic promoter is recognized and bounded by General Transcription Factors (GTF’s) and RNA Polymerase IIDNA polymerase III A prokaryotic enzyme with 5’ to 3’ Polymerase activity. The Hydrolysis of pyrophosphate
(PPi) drives the energy required to create the covalent
phosphodiester bond.A prokaryotic enzyme with 5’ to 3’ Polymerase activity.DNA polymerase is a multisubunit enzyme where the subunits of the enzyme form a ring that encircles and
moves along the template strand of the DNA, thus serving
as a sliding DNA clamp. Therefore the enzyme cannot
diffuse away from its task.DNA polymerase III has proofreading capabilities and therefore the error rate of RNA transcription
is 1 in 10 million bases (1X107).To ensure DNA replication fidelity, DNA polymerase III can edit its mistakes. DNA polymerase III has 3’ to 5’ Exonuclease activity.The 5’ to 3’ polymerase
activity then replaces it with the correct nucleotide.