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- Q1
Which DNA polymerase is mentioned in the text as having proofreading activity and being more thermostable than Taq polymerase?
Pfu polymerase
Escherichia coli polymerase
Taq polymerase
Reverse transcriptase
45s - Q2
In what type of studies is PCR routinely used to amplify genes from various sources, and commercially made primers are designed to base-pair with conserved regions of the gene?
Comparative or phylogenetic studies
Gene cloning studies
Forensic science studies
Clinical microbiology studies
45s - Q3
In which extension of the standard PCR procedure is reverse transcription PCR (RT-PCR) employed?
Forensic science
Cloning
Making cDNA from mRNA
Gene sequencing
45s - Q4
Why is PCR considered valuable in medical diagnostics in clinical microbiology laboratories?
It enables fast diagnosis of infectious diseases
It links crime scene evidence to suspects
It amplifies genes for cloning purposes
It detects gene expression
30s - Q5
What are single-stranded nucleic acids used in hybridization called?
Cloning vectors
Restriction enzymes
Probes
Primer
45s - Q6
Which technique uses probes of known sequence to hybridize with target DNA fragments separated by gel electrophoresis?
Recombineering
Northern blotting
FISH
Southern blotting
45s - Q7
What is the purpose of the multiple cloning site (MCS) in the plasmid pUC19?
To disrupt the lacZ gene
To linearize the vector
To facilitate ligation with DNA ligase
To provide resistance against ampicillin
45s - Q8
What is the role of a reporter gene in gene fusions?
To introduce mutations at specific sites.
To replace entire sections of a gene.
To disrupt the coding sequence of another gene.
To encode a protein that is easy to detect and assay.
45s - Q9
What is the key property of a reporter gene?
It is resistant to antibiotics.
It is easy to detect and assay.
It disrupts gene function.
It encodes a stable protein.
45s - Q10
What is the purpose of gene fusions in studying gene regulation?
To introduce mutations at specific sites
To test the effects of regulatory genes.
To replace entire sections of a gene.
To assay the expression of the natural gene product.
45s - Q11
The addition of RNase removes protein from extracted DNA.
falsetrueTrue or False30s - Q12
CTAB method makes use of SDS and proteinase to remove undigested DNA.
falsetrueTrue or False30s - Q13
Reverse and forward primers synthesize newcomplementary DNA strands.
falsetrueTrue or False30s - Q14
Nucleic acids possess positive phosphate backbones, hence, migration occurs to the negative electrode in electrophoresis.
falsetrueTrue or False30s - Q15
Visualization of DNA uses wavelength at 280 nm.
falsetrueTrue or False30s