FCM in Transfusion Medicine
Quiz by Sadeq K. Ali Al-Salait
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- Q1
What role does forward scatter (FSC) play in flow cytometry?
Measures cell size
Measures antigen expression intensity
Detects cell granularity
Quantifies cell proliferation
Identifies cell viability
30s - Q2
Which technology is used to detect light emission in flow cytometry?
Spectrophotometer
Scintillation counter
Infrared sensors
Photomultiplier tubes (PMTs)
Optical microscope
30s - Q3
Which of the following is a major application of flow cytometry in transfusion medicine?
Detection of feto-maternal hemorrhage (FMH)
HLA typing
Platelet transfusion matching
Routinely applicable for residual WBC counting
Donor selection in rare blood groups
30s - Q4
What threshold CD34+ cell count is recommended for efficient leukapheresis in HSCT?
100 × 10³/µl
20 × 10³/µl
5.0 × 10³/µl
50 × 10³/µl
30s - Q5
Which immune monitoring cells are analyzed in transplant patients using flow cytometry?
Red blood cells
Eosinophils
Neutrophils
T, B, and NK cells
Platelets
30s - Q6
What is the primary marker used to assess platelet activation during storage?
CD41b
CD62P (P-selectin)
CD41a
CD61a
30s - Q7
What does flow cytometry detect in DAT-negative AIHA?
Eluted Ab specificity
Absence of complement proteins
High platelet counts
RBC-bound autoantibodies
30s - Q8
Which method is more accurate for residual WBC counting in leukoreduced blood components?
Nageotte chamber
Microscopy
Flow cytometry
Manual hemocytometer
30s - Q9
What is the clinical relevance of anti-HPA-1a antibody detection?
Diagnosis of ITP
Assess platelet function
Detects kidney rejection
Diagnoses fetal and neonatal alloimmune thrombocytopenia (FNAIT)
30s - Q10
What is a key application of flow cytometry in solid organ transplantation?
Immune cell enumeration
Platelet crossmatching
Detection of donor-specific antibodies
Measuring red cell lifespan
30s