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Fluid Balance, Circulation and Oxygenation
Quiz by Julie Palmer
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SYI-1.D: Describe the structure and/ or function of subcellular components and organelles. ★ SYI-1.E: Explain how subcellular components and organelles contribute to the function of the cell. ★ SYI-1.F: Describe the structural features of a cell that allow organisms to capture, store, and use energy. ★ ENE-1.B: Explain the effect of surface area-to-volume ratios on the exchange of materials between cells or organisms and the environment. ★ ENE-1.C: Explain how specialized structures and strategies are used for the efficient exchange of molecules to the environment. ★ ENE-2.A: Describe the roles of each of the components of the cell membrane in maintaining the internal environment of the cell. ★ ENE-2.B: Describe the Fluid Mosaic Model of cell membranes. ★ ENE-2.C: Explain how the structure of biological membranes influences selective permeability. ★ ENE-2.D: Describe the role of the cell wall in maintaining cell structure and function. ★ ENE-2.E: Describe the mechanisms that organisms use to maintain solute and water balance. ★ ENE-2.F: Describe the mechanisms that organisms use to transport large molecules across the plasma membrane. ★ ENE-2.G: Explain how the structure of a molecule affects its ability to pass through the plasma membrane. ★ ENE-2.H: Explain how concentration gradients affect the ★ movement of molecules across membranes. ★ ENE-2.I: Explain how osmoregulatory mechanisms contribute to the health and survival of organisms. ★ ENE-2.J: Describe the processes that allow ions and other molecules to move across membranes. ★ ENE-2.K: Describe the membrane-bound structures of the eukaryotic cell. ★ ENE-2.L: Explain how internal membranes and membrane- bound organelles contribute to compartmentalization of eukaryotic cell functions. ★ EVO-1.A: Describe similarities and/or differences in compartmentalization between prokaryotic and eukaryotic cells. ★ EVO-1.B: Describe the relationship between the functions of endosymbiotic organelles and their free-living ancestral counterpartsPHOTOSYNTHESIS LIGHT DEPENDENT REACTION 1. Photosystem II (PSII) – Light Absorption & Water Splitting • Light energy (photons) excites electrons in chlorophyll molecules. • These high-energy electrons leave PSII and are passed into the electron transport chain (ETC). • Meanwhile, water molecules are split (photolysis) into: o O₂ (released as a by-product into the atmosphere) o H⁺ ions (protons, which build up inside the thylakoid) o Electrons (e⁻), which replace the ones lost by PSII. 2. Electron Transport Chain (ETC) • Excited electrons move through protein carriers embedded in the thylakoid membrane. • As they move, their energy pumps H⁺ ions into the thylakoid space, creating a proton gradient (high H⁺ inside, low outside). 3. ATP Production (ATP Synthase) • The buildup of H⁺ ions acts like a “waterfall” of potential energy. • These protons flow back across the membrane through ATP synthase, a protein complex that acts like a turbine. • This flow drives the conversion of ADP + Pi → ATP, which provides energy for the Calvin cycle. 4. Photosystem I (PSI) • Electrons arriving from the ETC enter PSI. • Sunlight excites them again, boosting them to a higher energy level. 5. NADPH Production • The energized electrons are transferred to NADP⁺. • Along with a proton (H⁺), this forms NADPH, another energy carrier. • NADPH is then delivered to the Calvin cycle to help build glucose. End Products of Light-Dependent Reactions: • ATP (energy source for Calvin cycle) • NADPH (reducing power for glucose synthesis) • O₂ (released into the atmosphere as waste) Light-Independent Reactions (Calvin Cycle) • These reactions do not directly require sunlight. • They occur in the stroma of the chloroplast (the fluid-filled space surrounding the thylakoids). • The inputs are ATP and NADPH (from light-dependent reactions) and CO₂ (from the atmosphere). • The outputs are glucose (C₆H₁₂O₆) and other carbohydrates. Think of the Calvin cycle as a factory that uses the energy and “raw materials” made in Stage I (ATP & NADPH) to build sugars. The 3 Main Steps of the Calvin Cycle 1. Carbon Fixation • CO₂ from the atmosphere enters the chloroplast and diffuses into the stroma. • Each CO₂ molecule attaches to a 5-carbon sugar called RuBP (ribulose-1,5-bisphosphate). • This reaction is catalyzed by the enzyme RuBisCO (Ribulose-1,5-bisphosphate carboxylase/oxygenase — the most abundant enzyme on Earth!). • The result is a short-lived 6-carbon compound, which immediately splits into two 3-carbon molecules called 3-PGA (3-phosphoglycerate). Summary: CO₂ + RuBP → 2 × 3-PGA 2. Reduction Phase • The 3-PGA molecules are “energized” and converted into G3P (glyceraldehyde-3-phosphate), a more energy-rich 3-carbon sugar. • This transformation requires: o ATP (provides energy) o NADPH (provides high-energy electrons and hydrogen atoms). • Some of the G3P molecules will eventually be combined to form glucose and other sugars. Summary: 3-PGA + ATP + NADPH → G3P 3. Regeneration of RuBP • Not all G3P molecules leave the cycle. Most of them are used to regenerate RuBP so the cycle can continue. • This regeneration also requires ATP. • For every 3 turns of the cycle, 5 G3P molecules are recycled to regenerate 3 molecules of RuBP. Summary: G3P + ATP → RuBP The Full Cycle Balance • To make one G3P molecule that can exit the cycle (and later form glucose), the cycle must run 3 times, fixing 3 molecules of CO₂. • To make one glucose molecule (C₆H₁₂O₆), the cycle must run 6 times (since glucose needs 6 carbon atoms). Inputs (for 1 glucose): • 6 CO₂ • 18 ATP • 12 NADPH Outputs: • 1 glucose (C₆H₁₂O₆) • 18 ADP + 18 Pi • 12 NADP⁺ Day vs Night Clarification • The Calvin Cycle is called light-independent, but that doesn’t mean it only happens at night. • It usually happens during the day because it depends on ATP and NADPH, which are only produced in light-dependent reactions (when sunlight is available). Simplified Analogy • Carbon fixation = The factory brings in CO₂ as raw material. • Reduction = Workers use energy (ATP & NADPH) to shape the raw material into useful products (G3P). • Regeneration = Some products are recycled to keep the factory running (RuBP is re-formed). • Output = After enough cycles, the factory produces glucose, the “food” of the plant.Bone growth and remodeling New bone development is balanced with bone resorption Haversian systems are continually being replaced Bone is resorbed from one area and added to another to meet changing stresses placed on it (e.g., weight, posture, fractures) Normal growth dependent on sufficient proteins, minerals, vitamins (A, C, D) and influenced by hormones (growth, thyroid, estrogen/testosterone) Bone is capable of repair because it contains osteoprogenitor cells in the periosteum, endosteum, and bone marrow and is very well vascularized Metabolic role of bone (contains 99% of body’s total calcium in crystals) Transfer calcium from crystals to interstitial fluid and into blood Hormonal Parathyroid - stimulates osteoblasts to secrete osteoclast-stimulating factor thus promoting resorption Calcitonin - inhibits osteoclast activityFluid and electrolytesFluid PowerFluid properties Fluid electrolyte and metabolic supportFluid Mechanics Quiz