
Higher 2.6 Biology
Quiz by Laura Lim
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- Q1How can wild strains of microorganisms be improved?
by having sterile conditions
by growing them in a fermentor
by mutagenesis or recombinant DNA technology
30s - Q2What can increase the rate of mutations?
exposure to UV light and other forms of radiation or mutagenic chemicals
putting it in sterile conditions
growing it with other microorganisms
30s - Q3What can be used as vectors for recombinant DNA technology?
artificial chromosomes and carbohydrates
recombinant plasmids and carbohydrates
recombinant plasmids and artificial chromosomes
30s - Q4What is a vector?
a method for transferring fatty acids into another cell
a method for transferring carbohydrates into another cell
a method for transferring a DNA molecule/carry foreign genetic information into another cell
30s - Q5When is it preferable to use artificial chromosomes rather than plasmids as vectors?
when smaller fragments of foreign DNA are required to be inserted
when larger fragments of foreign DNA are required to be inserted
when larger fragments of foreign fatty acids are required
30s - Q6What is the role of restriction endonucleases in recombinant DNA technology?
they cut open the nucleus and leave sticky ends
they cut open the cell and leave sticky ends
they cut open plasmids and to cut specific genes out of chromosomes leaving sticky ends
30s - Q7How are complementary stick ends produced?
when the same restriction endonuclease is used to cut open the plasmid and the gene from the chromosome
30s - Q8What is the enzyme ligase used for in recombinant DNA technology?
to seal in the fatty acid in the plasmid
to seal the gene in the plasmid
to remove the gene from the plasmid
30s - Q9What sites are on recombinant plasmids and artificial chromosomes?
restriction sites, protein sequences, an origin of replication and selectable markers
restriction sites, regulatory sequences, an origin of protein and selectable markers
restriction sites, regulatory sequences, an origin of replication and selectable markers
30s - Q10What does the restriction site contain?
target sequences of DNA where specific restriction endonucleases cut
allows self replication
site to control gene expression
30s - Q11What do regulatory sequences do?
control DNA replication
control membrane absorption
control gene expression
30s - Q12What is the origin of replication?
allows self-replication of the plasmid/artificial chromosome
allows proteing synthesis
allows gene expression
30s - Q13What are selectable markers?
genes present in the vector to ensure that only micro-organisms that take up the vector grow in the presence of the selective agent eg. antibiotic resistance genes
genes present in the nucleus to ensure that only micro-organisms that take up the vector grow in the presence of the selective agent eg. antibiotic resistance
genes present in the vector to ensure that all micro-organisms grow in the presence of the selective agent eg. antibiotic resistance genes
30s - Q14How can the prevention of recombinant microorganisms spreading in the environment occur?
genes are often introduced as a safety mechanisms to prevent the survival of microorganisms in an external environment
genes are often introduced as a safety mechanisms to prevent the survival of humans in an external environment
proteins are often introduced as a safety mechanisms to prevent the survival of microorganisms in an external environment
30s - Q15When would recombinant yeast cells be used?to produce active forms of the protein which are inactive in bacteria
to produce inactive forms of the protein which are inactive in bacteria
to produce active forms of the carbohydrate which are inactive in bacteria
30s