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Higher 2.6 Biology

Quiz by Laura Lim

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15 questions
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  • Q1
    How can wild strains of microorganisms be improved?

    by having sterile conditions 

    by growing them in a fermentor

    by mutagenesis or recombinant DNA technology

    30s
  • Q2
    What can increase the rate of mutations?

    exposure to UV light and other forms of radiation or mutagenic chemicals

    putting it in sterile conditions

    growing it with other microorganisms

    30s
  • Q3
    What can be used as vectors for recombinant DNA technology?

    artificial chromosomes and carbohydrates

    recombinant plasmids and carbohydrates

    recombinant plasmids and artificial chromosomes

    30s
  • Q4
    What is a vector?

    a method for transferring fatty acids into another cell

    a method for transferring carbohydrates into another cell

    a method for transferring a DNA molecule/carry foreign genetic information into another cell

    30s
  • Q5
    When is it preferable to use artificial chromosomes rather than plasmids as vectors?

    when smaller fragments of foreign DNA are required to be inserted

    when larger fragments of foreign DNA are required to be inserted

    when larger fragments of foreign fatty acids are required

    30s
  • Q6
    What is the role of restriction endonucleases in recombinant DNA technology?

    they cut open the nucleus and leave sticky ends

    they cut open the cell and leave sticky ends

    they cut open plasmids and to cut specific genes out of chromosomes leaving sticky ends

    30s
  • Q7
    How are complementary stick ends produced?

    when the same restriction endonuclease is used to cut open the plasmid and the gene from the chromosome

    30s
  • Q8
    What is the enzyme ligase used for in recombinant DNA technology?

    to seal in the fatty acid in the plasmid

    to seal the gene in the plasmid

    to remove the gene from the plasmid

    30s
  • Q9
    What sites are on recombinant plasmids and artificial chromosomes?

    restriction sites, protein sequences, an origin of replication and selectable markers

    restriction sites, regulatory sequences, an origin of protein and selectable markers

    restriction sites, regulatory sequences, an origin of replication and selectable markers

    30s
  • Q10
    What does the restriction site contain?

    target sequences of DNA where specific restriction endonucleases cut

    allows self replication

    site to control gene expression

    30s
  • Q11
    What do regulatory sequences do?

    control DNA replication

    control membrane absorption

    control gene expression

    30s
  • Q12
    What is the origin of replication?

    allows self-replication of the plasmid/artificial chromosome

    allows proteing synthesis

    allows gene expression

    30s
  • Q13
    What are selectable markers?

    genes present in the vector to ensure that only micro-organisms that take up the vector grow in the presence of the selective agent eg. antibiotic resistance genes 

    genes present in the nucleus to ensure that only micro-organisms that take up the vector grow in the presence of the selective agent eg. antibiotic resistance 

    genes present in the vector to ensure that all micro-organisms grow in the presence of the selective agent eg. antibiotic resistance genes 

    30s
  • Q14
    How can the prevention of recombinant microorganisms spreading in the environment occur?

    genes are often introduced as a safety mechanisms to prevent the survival of microorganisms in an external environment

    genes are often introduced as a safety mechanisms to prevent the survival of humans in an external environment

    proteins are often introduced as a safety mechanisms to prevent the survival of microorganisms in an external environment

    30s
  • Q15
    When would recombinant yeast cells be used?
    to produce active forms of the protein which are inactive in bacteria

    to produce inactive forms of the protein which are inactive in bacteria

    to produce active forms of the carbohydrate which are inactive in bacteria

    30s

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