The process of establishing laboratory conditions for the growth of microbes. These conditions are an attempt to mimic their microbe’s normal environment and promote growth; it is also performed on or in media.

Care must be taken to culture only the microbe of interest. ________ medium, devoid of all life forms, is inoculated.

The 3 types of cultures

_____ culture is a growth medium that contains only a single known species or types of microorganism.

______ culture is a container that holds two or more identified, easily differentiated species of microorganisms.

_______ culture was once pure or mixed ( with known species ) but has since had contaminants ( unwanted microbes of uncertain identity ) introduced to it.

nutrients for the growth of microbes; it can be classified to three properties

Media ( medium ) can be classified to three properties, except for one:

water based solution that does not solidify at temperature above freezing that tends to flow freely when the container is tilted.

growth appears as:

firmer than liquid media but not as firm as solid media; they do not flow freely and have a soft, clot like consistency at room temperature; they are used to examine the motility of bacteria and to provide a backdrop for visible reactions to occur.

solid media containing _____agar and is solid enough to remain in place when containers are tilted or inverted.

pure organic and inorganic compounds; have a molecular content specified by means of an exact formula.

match

matching

Matching

matching

a complex polysaccharide isolated from the alga Gelidium, is a critical tool in the microbiology lab; is flexible and moldable and it provides basic framework to hold moisture and nutrients. 

5 I's of microbiology correct sequence

to grow microorganisms

small sample of microbes

the introduction of an inoculum into media to culture microbes ; clinical specimens are obtained from body fluids, discharges, anatomical sites, or diseased tissue

 frequently as differential agents because many of them are pH indicators that change color in response to the production of an acid or a base.

Blood agar made by adding sterile sheep, horse, and rabbit blood is an example of

Nutrient agar and broth, brain heart infusion, and Trypticase soy agar (TSA) are examples of 

For growing bacteria that does not require oxygen

Media for isolating intestinal pathogens contain bile salts as selective agent.

widely used to grow fastidious streptococci and other pathogens

_______ bacteria requires growth factors and complex nutrients. 

_______ agar is a selective and differential culture medium for bacteria.

Selects against gram positive bacteria

to isolate a sample culture in a temperature-controlled environment which encourages growth.

usual temperature used in incubating

temperature controlled chamber; can control the content of atmospheric gasses such as oxygen and carbon dioxide.

following substance are present in enriched media, except

Differentiates between lactose-fermenting bacteria

separation of microbial cells by serial dilution or mechanical dispersion on solid media to create discrete colonies ( discrete mound of cells ) 

3 Separation techniques to accomplish isolation ; 

separation  technique that is useful when the sample contains a relatively low number of microorganisms or when the microorganisms are in clumps or aggregates.