
Recombinant DNA Technology and Molecular Cloning
Quiz by mb
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How is the joining of linear DNA fragments together with covalent bonds called?
Why type I and III restriction endonucleases are not useful for gene cloning?
There are four major classes of restriction endonucleases.
What process is used to purify plasmid DNA from crude cell lysates?
A technique used to move a particular DNA sequence from a parent vector to a destination vector.
The most widely used method for DNA sequencing is the Sanger.
Because the replication terminators (dideoxynucleoside triphosphates) lack the 3'-OH, they cannot form a phosphodiester bond with another nucleotide.
How many main pathways for repair of single base changes are there?
Once the mismatch is recognized, repair does not just involve the simple removal of the mispaired nucleotide. Instead, the mismatched DNA is destroyed and DNA replication starts over.
What process involves helical movement due to tracking along a groove of the DNA over short distances of less than 50 base pairs?