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Staining techniques

Quiz by N Raja

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10 questions
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  • Q1
    Which staining technique is commonly used to differentiate between Gram-positive and Gram-negative bacteria?
    Gram staining
    Negative staining
    Acid-fast staining
    Simple staining
    30s
  • Q2
    What type of stain is used to visualize cellular structures such as the nucleus and certain organelles in cells?
    Histological stain
    Fluorescent stain
    Counterstain
    Primary stain
    30s
  • Q3
    Which staining method is employed to observe the presence of mycobacteria?
    Endospore staining
    Negative staining
    Gram staining
    Acid-fast staining
    30s
  • Q4
    Which staining technique is primarily used to observe flagella in bacteria?
    Lactophenol cotton blue staining
    Flagella staining
    Crystal violet staining
    Giemsa staining
    30s
  • Q5
    What is the primary purpose of using a negative staining technique?
    To enhance fluorescence in microscopy
    To differentiate between Gram-positive and Gram-negative bacteria
    To stain the internal structures of a cell
    To visualize cell morphology and size without heat fixation
    30s
  • Q6
    In what scenario would you prefer using a simple stain over a differential stain?
    When looking for motility characteristics
    When distinguishing between different types of bacteria
    When identifying specific cell structures
    When you only need to observe cell shape and arrangement
    30s
  • Q7
    What is the role of counterstaining in a differential staining procedure?
    To fix the cells on the slide
    To provide contrast to the primary stain
    To initiate the staining process
    To remove excess dye
    30s
  • Q8
    Which staining technique would you use to observe the presence of endospores in bacteria?
    Capsule staining
    Gram staining
    Simple staining
    Endospore staining
    30s
  • Q9
    Which dye is commonly used as a primary stain in Gram staining?
    Methylene blue
    Safranin
    Crystal violet
    Eosin
    30s
  • Q10
    What is the primary function of a mordant in the staining process?
    To remove excess dye from the sample
    To fix the cells on the slide
    To provide color to the background
    To enhance the binding of the dye to the cells
    30s

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