Temperature Cooking HACCP

______ the sun set, the temperature began to drop. A) After B) While C) Since She studied all night for her test. ______, she was confident when she walked into class. A) Meanwhile B) As a result C) Before We arrived at the park early. ______, we set up our picnic and waited for the others to arrive. A) First B) Later C) Then The alarm rang loudly. ______, I jumped out of bed and rushed to get ready. A) Before B) Immediately C) Eventually The Wright brothers made their first successful flight in 1903. ______, air travel has advanced significantly. A) Since then B) Before C) Meanwhile Example Paragraphs (Choose the Best Transition Word) Cooking Instructions First, preheat the oven to 375°F. ______, mix the flour, sugar, and baking powder in a large bowl. Next, add the eggs and milk, stirring until smooth. (Options: Then, Before, While) Historical Events The Declaration of Independence was signed in 1776. ______, the United States officially became a separate nation from Britain. (Options: As a result, Before, However) Personal Narrative I packed my bags and headed to the airport. ______, I realized I had forgotten my passport at home. (Options: Later, Suddenly, Before) Daily Routine Every morning, I wake up at 6 AM. ______, I brush my teeth and make a cup of coffee. (Options: First, Afterward, Meanwhile) Science Explanation The caterpillar forms a chrysalis. ______, it undergoes metamorphosis and transforms into a butterfly. (Options: Eventually, Meanwhile, Before)

Write questions similar to ______ the sun set, the temperature began to drop. A) After B) While C) Since She studied all night for her test. ______, she was confident when she walked into class. A) Meanwhile B) As a result C) Before We arrived at the park early. ______, we set up our picnic and waited for the others to arrive. A) First B) Later C) Then The alarm rang loudly. ______, I jumped out of bed and rushed to get ready. A) Before B) Immediately C) Eventually The Wright brothers made their first successful flight in 1903. ______, air travel has advanced significantly. A) Since then B) Before C) Meanwhile Example Paragraphs (Choose the Best Transition Word) Cooking Instructions First, preheat the oven to 375°F. ______, mix the flour, sugar, and baking powder in a large bowl. Next, add the eggs and milk, stirring until smooth. (Options: Then, Before, While) Historical Events The Declaration of Independence was signed in 1776. ______, the United States officially became a separate nation from Britain. (Options: As a result, Before, However) Personal Narrative I packed my bags and headed to the airport. ______, I realized I had forgotten my passport at home. (Options: Later, Suddenly, Before) Daily Routine Every morning, I wake up at 6 AM. ______, I brush my teeth and make a cup of coffee. (Options: First, Afterward, Meanwhile) Science Explanation The caterpillar forms a chrysalis. ______, it undergoes metamorphosis and transforms into a butterfly. (Options: Eventually, Meanwhile, Before)

1. The following are ingredients in preparing pumping solution, EXCEPT; a. 5 kgs ham leg c. 1 cup saturated salt solution b. one tablespoon sugar d. 2 drops maplein 2. What is the value in grams of 6.4% refined salt if the amount of pork meat is 1 kilogram? a. 64 grams c. 640 grams b. 6.4 kilograms d. 6,400 grams 3. What is the main purpose of curing meat? a. To tenderize it c. To add color to it b. To prevent spoilage d. To increase its weight 4. What is the advantage of using pumping pickle over cover pickle and dry cure mixtures? a. It reduces the curing time. b. It enhances the flavor. c. It controls the concentration of salt. d. All of the above 5. What is the function of phosphate in curing solutions? a. It increases the water-holding capacity of the meat. b. It inhibits the growth of bacteria. c. It prevents oxidation of fat. d. It improves the texture of the meat. 6. What is the function of carrageenan in curing solutions? a. It acts as a thickener. b. It acts as a binder. c. It acts as a stabilizer. d. All of the above 7. In coloring salted eggs, how many teaspoons of vinegar must be added? a. 1 teaspoon b. 2 teaspoons c. 3 teaspoons d. 4 teaspoons 8. The following are factors that must be considered in packaging eggs. a. quality maintenance b. packaging design c. type of transport d. cost 9. In type 3 packaging material for eggs, what type of materials can these be made? a. burlap b. plastic c. paper board d. polystyrene 10. How many hours must be needed to bake the ham in an oven? a. 5 hours b. 3 hours c. 4 hours d. 2 hours 11. What is the ideal temperature of a smoke fish when storing it at home inside a refrigerator? a. 30℃ b. 38 ℃ c. 100 ℃ d. 50 ℃ 12. The following are ingredients for curing meat EXCEPT a. salt b. sugar c. vinegar d. cooking oil 13. What is the maximum period for commercial salted duck eggs? a. 18 days b. 21 days c. 14 days d. 30 days 14. What type of curing method is needed when fatty fish such as herring is being used? a. pickle curing b. dry curing c. cover pickle curing d. Pumping pickle curing 15. In making a pork ham, how many quarts of water must be needed to bring it to a boil? a. 2 quarts b. 3 quarts c. 4 quarts d. 5 quarts 16. In making a homemade skinless pork longganisa, what is the percentage of pork fats and lean meat? a. 20% fats, 80% lean meat b. 30% fats, 70% lean meat c. 40% fats, 60 lean meat d. 50% fats, 50% lean meat 17. It is a systematic procedure of producing a record for reference A. output C. documentation B. production report D. input 18. Anything produced, especially through a process, a product, a yield. A. documentation C. output B. input D. production report 19. It is the process of capturing data or translating information to a recording format. A. documentation C. production report B. reporting D. output 20. What is the value in grams of 6.4% refined salt if the amount of pork meat is 1 kilogram? a. 64 grams c. 640 grams b. 6.4 kilograms d. 6,400 grams 21. What is the difference between pumping pickle and cover pickle? a. Pumping pickle is injected into the meat, while cover pickle is poured over it. b. Pumping pickle is made with vinegar, while cover pickle is made with water. c. Pumping pickle is used for whole cuts of meat, while cover pickle is used for sliced meat. d. Pumping pickle is a dry mixture, while cover pickle is a liquid solution. 22. What is the main ingredient of dry cure mixtures? a. Sugar b. Salt c. Spices d. Phosphate 23. What is the function of vitamin C powder in curing solutions? a. It acts as an antioxidant. b. It enhances the color of the meat. c. It accelerates the curing reaction. d. All of the above 24. What is the ideal temperature for storing cured meat? a. Below 0°C b. Between 0°C and 4°C c. Between 4°C and 10°C d. Above 10°C 25. The following are advantages of packaging shell eggs EXCEPT. a. It protects against micro-organisms such as bacteria b. It prevents the loss of moisture. c. It protects the eggs from possible crushing while being handled, stored, or transported. d. It prolong the shelf life of the eggs. 26. How many pieces of eggs can be filled in a type 2 packaging materials, filler tray? a. 36 b. 12 c. 30 d. 24 27. How many hours must be needed in smoking ham? a. 10 to 19 hours b. 5 to 9 hours c. 15 to 24 hours d. 20 to 29 hours 28. It is a food packaging method that removes all air from a food-filled, plastic film package before sealing it. a. smoking b. drying c. vacuum packing d. weighing 29. Which is NOT TRUE about packaging a smoked fish? a. Sort cooled smoked fish according to size b. Pack or transfer smoked fish in bulk packaging materials by arranging the fish head and tail in any position. c. When the packaging material is nearly full, weigh the whole pack to check the product weight attained. d. Close or seal the packs. 30. The following nutrients can be found in an egg, EXCEPT. a. vitamins b. minerals c. omega 3 d. amino acids

**Tools and Equipment** * **Spatula:** Used for leveling ingredients and spreading fillings. * **Butter Knife:** Used for spreading butter, peanut butter, and cream cheese. * **Chef's Knives:** Come in various sizes, used for general cutting. * **Deli Knife:** Designed for thick sandwiches, with an offset blade for easy slicing. * **Lettuce Knife:** Serrated knife specifically designed for slicing lettuce. * **Paring Knife:** Small knife with a sharp blade, used for peeling and coring foods. * **Sandwich Knife:** Similar to a deli knife but shorter, used for slicing sandwiches. * **Serrated Knife:** Used for slicing bread with hard crusts and soft insides. * **Cutting Board:** A surface for cutting and preparing food. * **Mixing Bowls:** Used for mixing ingredients. * **Rubber Scraper:** Used for scraping down bowls and pans. * **Measuring Cups:** Used for measuring liquids and solids. * **Utility Tray:** Used for holding food in place. * **Strainer:** Used for separating liquids from solids. * **Mixing Spoon:** Used for combining ingredients. * **Can Opener:** Used for opening cans. * **Measuring Spoons:** Used for measuring small quantities of ingredients. * **Grills/Griddles:** Flat heated surfaces for direct cooking. * **Ovens:** Enclosed equipment for heating food with hot air or infrared radiation. * **Microwave Ovens:** Use microwave radiation to heat food. * **Salamanders:** Small broilers used for browning or glazing. * **Bread Toaster:** Used for toasting bread. * **Slicer:** Used for slicing food evenly. * **Chillers:** Used for chilling sandwiches and other foods. * **Freezer:** Used for storing food for extended periods. * **Refrigerator:** Used for storing food at a temperature below room temperature.

CARBOHYDRATES Carbohydrates are organic compounds composed of carbon, hydrogen, and oxygen in a ratio of about one carbon atom to two hydrogen atoms to one oxygen atom. The number of carbon atoms in a carbohydrate varies. Some carbohydrates serve as a source of energy. Other carbohydrates are used as structural materials. Carbohydrates can exist as monosaccharides, disaccharides, or polysaccharides. Monosaccharides A monomer of a carbohydrate is called a monosaccharide (MAHN-oh-SAK-uh-RIED). A monosaccharide—or simple sugar— contains carbon, hydrogen, and oxygen in a ratio of 1:2:1. The gen- eral formula for a monosaccharide is written as (CH2O)n, where n is any whole number from 3 to 8. For example, a six-carbon mono- saccharide, (CH2O)6, would have the formula C6H12O6. The most common monosaccharides are glucose, fructose, and galactose, as shown in Figure 3-6. Glucose is a main source of energy for cells. Fructose is found in fruits and is the sweetest of the monosaccharides. Galactose is found in milk. Notice in Figure 3-6 that glucose, fructose, and galactose have the same molecular formula, C6H12O6, but differing structures. The different structures determine the slightly different properties of the three compounds. Compounds like these sugars, with a single chemical formula but different structural forms, are called isomers (IE-soh-muhrz). SECTION 2 OBJECTIVES ● Distinguish between monosaccharides, disaccharides, and polysaccharides. ● Explain the relationship between amino acids and protein structure. ● Describe the induced fit model of enzyme action. ● Compare the structure and function of each of the different types of lipids. ● Compare the nucleic acids DNA and RNA. VOCABULARY carbohydrate monosaccharide disaccharide polysaccharide protein amino acid peptide bond polypeptide enzyme substrate active site lipid fatty acid phospholipid wax steroid nucleic acid deoxyribonucleic acid (DNA) ribonucleic acid (RNA) nucleotide C HO H C H OH C OH H C CH2OH H C H OH O Glucose C OH C O H OH C OH H CH2OH C H CH2OH Fructose C H HO C OH H C OH H C CH2OH H C H OH O Galactose Glucose, fructose, and galactose have the same chemical formula, but their structural differences result in different properties among the three compounds. FIGURE 3-6 Copyright © by Holt, Rinehart and Winston. All rights reserved. 56 CHAPTER 3 Disaccharides and Polysaccharides In living things, two monosaccharides can combine in a condensa- tion reaction to form a double sugar, or disaccharide (die-SAK-e-RIED). For example in Figure 3-4, the monosaccharides fructose and glu- cose can combine to form the disaccharide sucrose. A polysaccharide is a complex molecule composed of three or more monosaccharides. Animals store glucose in the form of the polysaccharide glycogen. Glycogen consists of hundreds of glucose molecules strung together in a highly branched chain. Much of the glucose that comes from food is ultimately stored in your liver and muscles as glycogen and is ready to be used for quick energy. Plants store glucose molecules in the form of the polysaccha- ride starch. Starch molecules have two basic forms—highly branched chains that are similar to glycogen and long, coiled, unbranched chains. Plants also make a large polysaccharide called cellulose. Cellulose, which gives strength and rigidity to plant cells, makes up about 50 percent of wood. In a single cellu- lose molecule, thousands of glucose monomers are linked in long, straight chains. These chains tend to form hydrogen bonds with each other. The resulting structure is strong and can be broken down by hydrolysis only under certain conditions. PROTEINS Proteins are organic compounds composed mainly of carbon, hydrogen, oxygen, and nitrogen. Like most of the other biological macromolecules, proteins are formed from the linkage of monomers called amino acids. Hair and horns, as shown in Figure 3-7a, are made mostly of proteins, as are skin, muscles and many biological catalysts (enzymes). Amino Acids There are 20 different amino acids, and all share a basic structure. As Figure 3-7b shows, each amino acid contains a central carbon atom covalently bonded to four other atoms or functional groups. A single hydrogen atom, highlighted in blue in the illustration, bonds at one site. A carboxyl group, —COOH, highlighted in green, bonds at a second site. An amino group, —NH2, highlighted in yel- low, bonds at a third site. A side chain called the R group, high- lighted in red, bonds at the fourth site. The main difference among the different amino acids is in their R groups. The R group can be complex or it can be simple, such as the CH3 group shown in the amino acid alanine in Figure 3-7b. The differences among the amino acid R groups gives different proteins very different shapes. The different shapes allow pro- teins to carry out many different activities in living things. Amino acids are commonly shown in a simplified way such as balls, as shown in Figure 3-7c. (a) Many structures, such as hair and horns are made of proteins. (b) Proteins are made up of amino acids. Amino acids differ only in the type of R group (shown in red) they carry. Polar R groups can dissolve in water, but nonpolar R groups cannot. (c) Amino acids have complex structures, so, in this and other textbooks, they are often simplified into balls. FIGURE 3-7 (b) Alanine (an amino acid) (c) Simplified version of amino acid CH3 H N OH C C H O H (a) Copyright © by Holt, Rinehart and Winston. All rights reserved. BIOCHEMISTRY 57 H H N C C OH H O H CH3 H2O Glycine Alanine H N OH C C H O H H H N C C H O H CH3 N OH C C H O H (a) (b) (a) The peptide bond (shaded blue) that binds amino acids together to form a polypeptide results from a condensation reaction that produces water. (b) Poly- peptides are commonly shown as a string of balls in this textbook and elsewhere. Each ball represents an amino acid. FIGURE 3-8 Substrate Products Enzyme 1 2 3 In the induced fit model of enzyme action, the enzyme can attach only to a substrate (reactant) with a specific shape. The enzyme then changes and reduces the activation energy of the reaction so reactants can become products. The enzyme is unchanged and is available to be used again. 3 2 1 FIGURE 3-9 Dipeptides and Polypeptides Figure 3-8a shows how two amino acids bond to form a dipeptide (die-PEP-TIED). In this condensation reaction, the two amino acids form a covalent bond, called a peptide bond (shaded in blue in Figure 3-8a) and release a water molecule. Amino acids often form very long chains called polypeptides (PAHL-i-PEP-TIEDZ). Proteins are composed of one or more polypep- tides. Some proteins are very large molecules, containing hun- dreds of amino acids. Often, these long proteins are bent and folded upon themselves as a result of interactions—such as hydrogen bonding—between individual amino acids. Protein shape can also be influenced by conditions such as temperature and the type of solvent in which a protein is dissolved. For exam- ple, cooking an egg changes the shape of proteins in the egg white. The firm, opaque result is very different from the initial clear, runny material. Enzymes Enzymes—RNA or protein molecules that act as biological catalysts—are essential for the functioning of any cell. Many enzymes are proteins. Figure 3-9 shows an induced fit model of enzyme action. Enzyme reactions depend on a physical fit between the enzyme molecule and its specific substrate, the reactant being catalyzed. Notice that the enzyme has folds, or an active site, with a shape that allows the substrate to fit into the active site. An enzyme acts only on a specific substrate because only that substrate fits into its active site. The linkage of the enzyme and substrate causes a slight change in the enzyme’s shape. The change in the enzyme’s shape weakens some chemical bonds in the substrate, which is one way that enzymes reduce activation energy, the energy needed to start the reaction. After the reaction, the enzyme releases the products. Like any catalyst, the enzyme itself is unchanged, so it can be used many times. An enzyme may not work if its environment is changed. For example, change in temperature or pH can cause a change in the shape of the enzyme or the substrate. If such a change happens, the reaction that the enzyme would have catalyzed cannot occur.

Got it ✅ — you want all the topics (States of Matter → Temperature → Thermal Energy → Pressure → Gas Laws, etc.) written in the same structured style (definitions, everyday examples, non-examples, short story, daily life connections, MCQs, SAQs). Here’s a full structured lesson flow, starting with States of Matter as you requested: --- 🧊 States of Matter Definition Matter exists in three main states: Solid: Definite shape & volume, particles tightly packed, vibrate in place. Liquid: Definite volume but takes the shape of its container, particles slide past one another. Gas: No definite shape or volume, particles move freely and spread out. Everyday Examples Solid: Ice cubes, table, book. Liquid: Water, milk, juice. Gas: Air in a balloon, perfume spreading, steam. Non-Examples Honey is not a solid → it flows → liquid. A rock is not a liquid → it’s rigid → solid. Water in a closed bottle is not a gas → it stays liquid. Short Story You buy a soda on a hot day: Ice cubes (solid) keep it cold. They melt into liquid water. Bubbles rise as gas carbon dioxide escapes. Everyday Life Connections Freezing water into ice. Boiling soup on the stove. Smell of perfume spreading across a room. MCQs 1. Which state has particles vibrating in place? a) Solid ✅ b) Liquid c) Gas d) Plasma 2. Soda fizzing when opened is: a) Liquid diffusion b) Gas release ✅ c) Solid melting d) Condensation SAQ (Multi-step) You leave an ice cream outside: a) What state does it start in? b) What happens as it melts? c) If left longer, what phase change might occur? d) Which type of energy increases? --- 🌡 Temperature Definition Indicates average kinetic energy of particles. Measured with a thermometer. Heat flows between objects of different temperature. Everyday Examples Fever check with a thermometer. Ice cube cooling a drink. Why metal feels colder than wood at room temperature. Short Story A hot pizza slice cools when left on the table: heat flows from pizza (high T) to air (low T). MCQ Which is true about temperature? a) It measures total energy b) It measures average kinetic energy ✅ c) It is the same as heat d) It doesn’t affect particle motion --- 🔥 Thermal Energy Definition Total of all kinetic and potential energy of atoms in an object. Everyday Examples Large pot of warm soup has more thermal energy than a small hot cup. Heating water → particles move faster. Ice pack absorbs thermal energy from skin. Short Story In winter, sitting near a heater warms you up because air molecules gain kinetic energy and transfer it. MCQ At absolute zero: a) Particles vibrate slowly b) Particles move randomly c) Particles have no movement ✅ d) Particles expand --- ⚡ Kinetic vs Potential Energy Definition Kinetic energy: energy of motion (vibrating, flowing, diffusing). Potential energy: stored in positions/forces (attractions between particles). Everyday Examples Steam in cooker: high kinetic energy. Rubber band stretched: potential energy. Short Story A bouncing ball → kinetic while moving, potential at the top of its bounce. --- 💨 Pressure Definition Force per unit area on a surface. Everyday Examples Drinking with a straw. Bicycle tires feel hard due to air pressure. Bed of nails → force spread out, less pressure. Short Story When you open a soda bottle, pressure is released → fizzing sound and bubbles. --- 🔄 Gas Laws (Thermal Expansion & Charles’ Law) Definition At constant pressure, gas volume ∝ absolute temperature. Everyday Examples Balloon expands in sunlight. Hot air balloon rises. Tires inflate slightly after driving. Short Story A sealed chips bag puffs up on an airplane as air pressure outside decreases. MCQ According to Charles’ Law: a) Volume decreases as temperature increases b) Volume increases as temperature increases ✅ c) Volume is independent of temperature d) Volume and temperature are unrelated --- ✅ This flow covers all your slides in the same Prezi-style (definitions, examples, non-examples, story, life connections, questions). Do you want me to now add full sets of practice (10 True/False, 10 Matching, 10 Write the Term, etc.) for each section, so you’ll have a complete question bank along with the lesson flow?

Lide 1: Introduction to Bioreactor A bioreactor is a vessel used for growing microorganisms, plant or animal cells Provides controlled conditions for biological reactions Maintains optimum pH, temperature, oxygen, and nutrients Widely used in fermentation, enzyme, vaccine, and antibiotic production Ensures sterile and aseptic environment Scale ranges from laboratory to industrial production Slide 2: Basic Design Requirements of a Bioreactor Must be constructed with non-toxic, corrosion-resistant materials Should allow effective mixing and mass transfer Provision for sterilization (in situ sterilization) Must maintain uniform temperature and pH Easy sampling without contamination Should support scalability and automation Slide 3: Materials Used in Bioreactor Construction Stainless steel (SS-316) for industrial bioreactors Glass for laboratory-scale bioreactors Plastic (polycarbonate) for disposable bioreactors Materials must withstand heat and pressure Should be smooth to prevent microbial attachment Resistant to chemicals and cleaning agents Slide 4: Main Parts of a Bioreactor Vessel: holds the culture medium and microorganisms Agitator (impeller): provides mixing Sparger: supplies sterile air Baffles: prevent vortex formation Sensors: monitor pH, temperature, dissolved oxygen Ports: used for inoculation, sampling, and feeding Slide 5: Agitation System Ensures uniform mixing of nutrients and cells Improves oxygen transfer rate Common impellers: Rushton turbine, marine propeller Speed controlled by motor Prevents settling of cells Affects shear stress on cells Slide 6: Aeration System Supplies oxygen for aerobic fermentation Air introduced through sparger Types of spargers: ring, nozzle, sintered Maintains dissolved oxygen concentration Air is filtered for sterility Essential for high cell density cultures Slide 7: Temperature and pH Control Temperature controlled by heating/cooling jackets pH maintained using acid or alkali addition Sensors continuously monitor parameters Automated control systems used Ensures optimal microbial growth Prevents enzyme denaturation Slide 8: Foam Control System Foam formed due to protein and agitation Excess foam reduces oxygen transfer Mechanical foam breakers used Chemical antifoam agents added Foam sensor detects foam formation Maintains efficient fermentation Slide 9: Types of Bioreactors – Based on Mode of Operation Batch bioreactor Fed-batch bioreactor Continuous bioreactor Choice depends on product type Widely used in industrial fermentation Controls productivity and yield Slide 10: Batch Bioreactor All nutrients added at the beginning No addition or removal during process Simple and easy to operate Low risk of contamination Used for antibiotics and enzymes Limited control over nutrient depletion Slide 11: Fed-Batch Bioreactor Nutrients added during fermentation Prevents substrate inhibition High product yield Widely used in industrial fermentation Allows better control of growth rate Used in insulin and enzyme production Slide 12: Continuous Bioreactor Fresh medium continuously added Culture removed at same rate Maintains steady-state conditions High productivity Risk of contamination is high Used in wastewater treatment and SCP production Slide 13: Types of Bioreactors – Based on Design Stirred tank bioreactor Airlift bioreactor Bubble column bioreactor Packed bed bioreactor Fluidized bed bioreactor Photobioreactor Slide 14: Stirred Tank Bioreactor (STR) Most commonly used bioreactor Mechanical agitation using impellers Suitable for aerobic fermentation Excellent mixing and oxygen transfer Used for bacteria and fungi Easy scale-up Slide 15: Airlift Bioreactor Mixing achieved by air circulation No mechanical agitator Low shear stress Energy efficient Suitable for shear-sensitive cells Used in wastewater treatment Slide 16: Bubble Column Bioreactor Air bubbles provide mixing Simple design and low cost No moving parts Limited mixing efficiency Used for microbial fermentation Suitable for large-scale operations Slide 17: Packed Bed Bioreactor Contains immobilized cells or enzymes Substrate flows through packed matrix High cell density Used in continuous processes Limited oxygen transfer Used in enzyme and wastewater treatment Slide 18: Fluidized Bed Bioreactor Immobilized particles kept in suspension Better mass transfer than packed bed Reduced clogging Suitable for continuous operation Used in biotransformations Higher operational complexity Slide 19: Photobioreactor Designed for photosynthetic organisms Provides light source Used for algae and cyanobacteria Controls light, CO₂, and temperature Used in biofuel and pigment production Can be tubular or flat-plate design Slide 20: Applications of Bioreactors Production of antibiotics and vaccines Enzyme and organic acid production Single cell protein production Wastewater treatment Biofertilizer and biopesticide production Biopharmaceutical manufacturing

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