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Waste food in restaurants
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Food waste in restaurants
⢠There are two groups of animals which are important in agriculture. ⢠The groups are domestic and wild animals. 1. Domestic animals ⢠These are animals which are trained to live with people in their homes. ⢠Some examples includes : Uses of domestic animals ⢠Source of food, for example milk, eggs and meat. ⢠For cultural purposes, for example paying lobola. ⢠Some are used for transport and labour. ⢠Domestic animals can also be a source of income. 2 . Wild animals ⢠These are animals which are found in game reserves and in the forests ⢠They are also called game animals. ⢠Examples of wild animals are: Uses of wild animals ⢠Some of the wild animals give us meat, hides and ivory. ⢠Wild animals attract visitors from other countries, so the country gets money. Wednesday 06 September 2023 Exercise: Domestic animals 1. What is a domestic animal? [2] 2. What is a wild animal? [2] 3. Name any 4 domestic animals that you know. [4] 4. State any 2 uses of domestic animals. [2] 5. Name any 3 wild animals that you know. [3] 6. Give 2 uses of wild ani mals. [2] 7. Wild animals can also be called animals. [1] ⢠Animals, like human beings need good food to help them to grow and reproduce. ⢠The main sources of food for animals include stock feeds, pastures, veld grass, crop remains and cereal grains. ⢠These foods contain the most needed essential nutrients. Nutrient Function carbohydrates Gives energy Fats Give energy and warmth Proteins Helps in growth and repair of body parts Minerals Help in the formation of bones and teeth Vitamins Help develop good sight, improve fertility and help animals fight diseases Water Transports food in the body, cools the body and remove waste from the body. ⢠Livestock are domesticated animals that are kept for food, use or for sale. ⢠Small livestock has many uses. ⢠They are a good source of food. ⢠They also give us manure to use in gardens. ⢠We get clothes and medicine as well from small livestock ⢠Examples of small livestock are rabbits, sheep, goats and all form of poultry Poultry ⢠All animals that are kept by farmers which have wings and feathers and lay eggs are called poultry. ⢠They are a good source of white meat, eggs and manure. ⢠They are also a source of income when we well them and their products. ⢠Poultry includes chicken, guinea fowl, ducks, turkey, pigeons and quail birds. Rabbits ⢠Rabbits have 3 known uses which are: ļ¼They are kept for meat ļ¼Kept for pelts. (pelt is animal skin used to make blankets, hats and jackets) ļ¼They are used at agricultural shows ⢠Rabbits are cheap to buy, easy to keep and feed. ⢠They take about 4 months to mature. ⢠Rabbits are fed using green vegetables and rabbit pellets. Wednesday 20 September 2023 Small livestock 1. Give 3 examples of small livestock. [3] 2. State 3 things that are provided by small livestock. [3] 3. What are the 2 uses of rabbits? [2] 4. Pelts are used to make _________________ [1] 5. State any 4 examples poultry. [4] 6. Rabbits take ___________ months to mature. [1] 7. What is poultry? ⢠Apiculture is the keeping of bees in order for them to produce honey for sale. ⢠Apiculture is very important because: (i) Provides honey - a valuable nutritional food (ii) Provides bees wax - which has many uses in industry Uses of wax ļ¼For making candles ļ¼Polish furniture ļ¼Make crayons ļ¼Prevent tools from rusting (iii) Honey bees are excellent pollinating agents, thus increasing agricultural yields. BEE COLONY Inhabitants of the bee colony and their roles ⢠A honey bee colony typically consists of three kinds of adult bees: ļ¼ļ workers, drones and the queen 1. Workers ⢠Workers are the smallest and constitute the majority of bees occupying the colony. ⢠They do not lay eggs. ⢠Workers have specialized structures, such as brood food glands, scent glands, wax glands, and pollen baskets. ⢠these allow them to perform all the labors of the hive. Roles of the worker bees ļ¼they forage for nectar, pollen, water, and plant sap. ļ¼They clean and polish the cells. ļ¼feed the brood. ļ¼care for the queen. ļ¼remove debris. ļ¼handle incoming nectar. ļ¼build beeswax combs. ļ¼guard the entrance. 2. Drones ⢠Drones (male bees) are the largest bees in the colony. ⢠They are generally present only during late spring and summer. ⢠The droneās head is much larger than that of either the queen or worker. ⢠Drones have no stinger, pollen baskets, or wax glands. ⢠Their main function is to mate with the queen. 3 . Queen ⢠Each colony has only one queen. ⢠The queen is the largest of the bees in a bee colony. ⢠The Queen Bee plays a vital role in the hive because she is the only female with fully developed ovaries. ⢠She produces both fertilized and unfertilized eggs. ⢠Queens lay the greatest number of eggs in the spring and early summer. ⢠The queen also produce chemical scents that help regulate the unity of the colony. 1. What is apiculture? [2] 2. Give 3 reasons why apiculture is important in Zimbabwe. [3] 3. Name the 3 inhabitants of the bee colony. [3] 4. Briefly explain the roles of each inhabitant named in number 3. ⢠Apart from using hand tools, farmers also use some farm implements and machinery to carry out their field work. ⢠Machines help farmers do their work more easily and quickly. ⢠The most common implements used by farmers to grow, harvest and transport their produces are: ļ¼ mouldboard plough ļ¼Cultivator ļ¼Scotch cart ļ¼Harrow ļ¼Planter ļ¼Maize sheller ļ¼ combine harvesters ļ¼Boom sprayers Disc harrow Spike toothed harrow KNAPSACK SPRAYER ⢠Farming is a business. ⢠Communal farmers grow crops and keep animal mainly for their own use. ⢠If there is any extra they sell to get money. ⢠Commercial farmers grow crops and keep animals for sale. ⢠Crops and animals produced are called farm produce. ⢠There are places were farmers have to sell their produce. ⢠Farmers can take their produce to local markets. ⢠A market is a place where buying and selling occurs. ⢠Some of the local markets includes: ļ¼A shopping centre ļ¼A school ļ¼A nearest bus stop ļ¼A local village ļ¼A school ļ¼Local Grain Marketing Board depot (GMB) Types of farm produce ļ¼Beef ļ¼Fruits ļ¼Mutton ļ¼Eggs ļ¼Vegetables ļ¼Pork ļ¼Milk ļ¼Chicken ļ¼Cereals/grains ļ¼beans
What is a Plant Cell? Plant cells are eukaryotic cells that vary in several fundamental factors from other eukaryotic organisms. Both plant and animal cells contain a nucleus along with similar organelles. One of the distinctive aspects of a plant cell is the presence of a cell wall outside the cell membrane. Plant Cell Structure Just like different organs within the body, plant cell structure includes various components known as cell organelles that perform different functions to sustain itself. These organelles include: Cell Wall It is a rigid layer which is composed of polysaccharides cellulose, pectin and hemicellulose. It is located outside the cell membrane. It also comprises glycoproteins and polymers such as lignin, cutin, or suberin. The primary function of the cell wall is to protect and provide structural support to the cell. The plant cell wall is also involved in protecting the cell against mechanical stress and providing form and structure to the cell. It also filters the molecules passing in and out of it. The formation of the cell wall is guided by microtubules. It consists of three layers, namely, primary, secondary and the middle lamella. The primary cell wall is formed by cellulose laid down by enzymes. Cell membrane It is the semi-permeable membrane that is present within the cell wall. It is composed of a thin layer of protein and fat. The cell membrane plays an important role in regulating the entry and exit of specific substances within the cell. For instance, cell membrane keeps toxins from entering inside, while nutrients and essential minerals are transported across. Nucleus The nucleus is a membrane-bound structure that is present only in eukaryotic cells. The vital function of a nucleus is to store DNA or hereditary information required for cell division, metabolism and growth. 1. Nucleolus: It manufactures cellsā protein-producing structures and ribosomes. 2. Nucleopore: Nuclear membrane is perforated with holes called nucleopore that allow proteins and nucleic acids to pass through. Plastids They are membrane-bound organelles that have their own DNA. They are necessary to store starch and to carry out the process of photosynthesis. It is also used in the synthesis of many molecules, which form the building blocks of the cell. Some of the vital types of plastids and their functions are stated below: Leucoplasts They are found in the non-photosynthetic tissue of plants. They are used for the storage of protein, lipid and starch. Chromoplasts They are heterogeneous, colored plastid which is responsible for pigment synthesis and for storage in photosynthetic eukaryotic organisms. Chromoplasts have red-, orange- and yellow-colored pigments which provide color to all ripe fruits and flowers. Central Vacuole It occupies around 30% of the cellās volume in a mature plant cell. Tonoplast is a membrane that surrounds the central vacuole. The vital function of the central vacuole apart from storage is to sustain turgor pressure against the cell wall. The central vacuole consists of cell sap. It is a mixture of salts, enzymes and other substances. Golgi Apparatus They are found in all eukaryotic cells, which are involved in distributing synthesized macromolecules to various parts of the cell. Ribosomes They are the smallest membrane-bound organelles which comprise RNA and protein. They are the sites for protein synthesis, hence, also referred to as the protein factories of the cell. Mitochondria They are the double-membraned organelles found in the cytoplasm of all eukaryotic cells. They provide energy by breaking down carbohydrate and sugar molecules, hence they are also referred to as the āPowerhouse of the cell.ā Lysosome Lysosomes are called suicidal bags as they hold digestive enzymes in an enclosed membrane. They perform the function of cellular waste disposal by digesting worn-out organelles, food particles and foreign bodies in the cell. In plants, the role of lysosomes is undertaken by the vacuoles. Chloroplasts It is an elongated organelle enclosed by phospholipid membrane. The chloroplast is shaped like a disc and the stroma is the fluid within the chloroplast that comprises a circular DNA. Each chloroplast contains a green colored pigment called chlorophyll required for the process of photosynthesis. The chlorophyll absorbs light energy from the sun and uses it to transform carbon dioxide and water into glucose. Structure of Chloroplast Chloroplasts are found in all higher plants. It is oval or biconvex, found within the mesophyll of the plant cell. The size of the chloroplast usually varies between 4-6 µm in diameter and 1-3 µm in thickness. They are double-membrane organelle with the presence of outer, inner and intermembrane space. There are two distinct regions present inside a chloroplast known as the grana and stroma. ⢠Grana are made up of stacks of disc-shaped structures known as thylakoids or lamellae. The granum of the chloroplast consists of chlorophyll pigments and are the functional units of chloroplasts. ⢠Stroma is the homogenous matrix which contains grana and is similar to the cytoplasm in cells in which all the organelles are embedded. Stroma also contains various enzymes, DNA, ribosomes, and other substances. Stroma lamellae function by connecting the stacks of thylakoid sacs or grana. The chloroplast structure consists of the following parts: Membrane Envelope It comprises inner and outer lipid bilayer membranes. The inner membrane separates the stroma from the intermembrane space. Intermembrane Space The space between inner and outer membranes. Thylakoid System (Lamellae) The system is suspended in the stroma. It is a collection of membranous sacs called thylakoids or lamellae. The green colored pigments called chlorophyll are found in the thylakoid membranes. It is the sight for the process of light-dependent reactions of the photosynthesis process. The thylakoids are arranged in stacks known as grana and each granum contains around 10-20 thylakoids. Stroma It is a colorless, alkaline, aqueous, protein-rich fluid present within the inner membrane of the chloroplast present surrounding the grana. Grana Stack of lamellae in plastids is known as grana. These are the sites of conversion of light energy into chemical energy. Chlorophyll It is a green photosynthetic pigment that helps in the process of photosynthesis. Functions of Chloroplast Following are the important chloroplast functions: ⢠The most important function of the chloroplast is to synthesize food by the process of photosynthesis. ⢠Absorbs light energy and converts it into chemical energy. ⢠Chloroplast has a structure called chlorophyll which functions by trapping the solar energy and is used for the synthesis of food in all green plants. ⢠Produces NADPH and molecular oxygen (O 2 ) by photolysis of water. ⢠Produces ATP ā Adenosine triphosphate by the process of photosynthesis. ⢠The carbon dioxide (CO2) obtained from the air is used to generate carbon and sugar during the Calvin Cycle or dark reaction of photosynthesis. Mitochondria āMitochondria are membrane-bound organelles present in the cytoplasm of all eukaryotic cells, that produce adenosine triphosphate (ATP), the main energy molecule used by the cell.ā What are Mitochondria? Popularly known as the āPowerhouse of the cell,ā mitochondria (singular: mitochondrion) are a double membrane-bound organelle found in most eukaryotic organisms. They are found inside the cytoplasm and essentially function as the cellās ādigestive system.ā They play a major role in breaking down nutrients and generating energy-rich molecules for the cell. Many of the biochemical reactions involved in cellular respiration take place within the mitochondria. The term āmitochondrionā is derived from the Greek words āmitosā and āchondrionā which means āthreadā and āgranules-likeā, respectively. It was first described by a German pathologist named Richard Altmann in the year 1890. Structure of Mitochondria ⢠The mitochondrion is a double-membraned, rod-shaped structure found in both plant and animal cell. ⢠Its size ranges from 0.5 to 1.0 micrometers in diameter. ⢠The structure comprises an outer membrane, an inner membrane, and a gel-like material called the matrix. ⢠The outer membrane and the inner membrane are made of proteins and phospholipid layers separated by the intermembrane space. ⢠The outer membrane covers the surface of the mitochondrion and has a large number of special proteins known as porins. Cristae The inner membrane of mitochondria is rather complex in structure. It has many folds that form a layered structure called cristae, and this helps in increasing the surface area inside the organelle. The cristae and the proteins of the inner membrane aid in the production of ATP molecules. The inner mitochondrial membrane is strictly permeable only to oxygen and ATP molecules. A number of chemical reactions take place within the inner membrane of mitochondria. Mitochondrial Matrix The mitochondrial matrix is a viscous fluid that contains a mixture of enzymes and proteins. It also comprises ribosomes, inorganic ions, mitochondrial DNA, nucleotide cofactors, and organic molecules. The enzymes present in the matrix play an important role in the synthesis of ATP molecules. Functions of Mitochondria The most important function of mitochondria is to produce energy through the process of oxidative phosphorylation. It is also involved in the following process: 1. Regulates the metabolic activity of the cell 2. Promotes the growth of new cells and cell multiplication 3. Helps in detoxifying ammonia in the liver cells 4. Plays an important role in apoptosis or programmed cell death 5. Responsible for building certain parts of the blood and various hormones like testosterone and estrogen 6. Helps in maintaining an adequate concentration of calcium ions within the compartments of the cell 7. It is also involved in various cellular activities like cellular differentiation, cell signaling, cell senescence, controlling the cell cycle and in cell growth. Disorders Associated with Mitochondria Any irregularity in the way mitochondria function can directly affect human health, but often, it is difficult to identify because symptoms differ from person to person. Disorders of the mitochondria can be quite severe; in some cases, they can even cause an organ to fail.
All animals, most protists, all fungi, and many bacteria are het- erotrophs. Unlike autotrophs, heterotrophs cannot manufacture their own food. Instead, they get energy by eating other organisms or organic wastes. Ecologically speaking, heterotrophs are consumers. They obtain energy by consuming organic molecules made by other organisms. Consumers can be grouped according to the type of food they eat. Herbivores eat producers. An antelope that eats grass is a herbivore. Carnivores eat other consumers. Lions, cobras, and praying mantises are examples of carnivores. Omnivores eat both producers and consumers. The grizzly bear, whose diet ranges from berries to salmon, is an omnivore. Detritivores (dee-TRIET-uh-VAWRZ) are consumers that feed on the āgarbageā of an ecosystem. This waste, or detritus, includes organisms that have recently died, fallen leaves, and animal wastes. The vulture shown in Figure 18-8 is a detritivore. Many bacteria and fungi are detritivores that cause decay by breaking down complex molecules into simpler molecules. So, they are specifically called decomposers. Some of the molecules released during decay are absorbed by the decomposers, and some are returned to the soil or water. Decomposers make the nutrients that were contained in detritus available again to the autotrophs in the ecosystem. Thus, the process of decomposition recycles chemical nutrients. Copyright Ā© by Holt, Rinehart and Winston. All rights reserved. 368 CHAPTER 18 ENERGY FLOW When one organism eats another, molecules are metabolized and energy is transferred. As a result, energy flows through an ecosystem, moving from producers to consumers. One way to follow the pattern of energy flow is to group organisms in an ecosystem based on how they obtain energy. An organismās trophic (TRAHF-ik) level indicates the organismās position in a sequence of energy transfers. For exam- ple, all producers belong to the first trophic level. Herbivores belong to the second trophic level, and the predators belong to the third level. Most terrestrial ecosystems have only three or four trophic lev- els, whereas marine ecosystems often have more. Food Chains and Food Webs A food chain is a single pathway of feeding relationships among organisms in an ecosystem that results in energy transfer. A food chain may begin with grass, which is a primary producer. The chain may continue with a consumer of grass seedsāa meadow mouse. Next, a carnivorous snake may kill and eat the mouse. A hawk then may eat the snake, as shown in Figure 18-9. The feeding relationships in an ecosystem are usually too com- plex to be represented by a single food chain. Many consumers eat more than one type of food. In addition, more than one species of consumer may feed on the same organism. Many food chains inter- link, and a diagram of the feeding relationships among all the organisms in an ecosystem would resemble a web, as shown in Figure 18-10. For this reason, the interrelated food chains in an ecosystem are called a food web.
It is a basic unit of life in the smallest structure capable of basic life processes such as taking and nutrients expelling waste and reproducing is sometimes called the building block of life. a. Organ c. Cell b. DNA d. Nucleus 2. It surrounds the cell that separates the material outside the cell from the material inside the cell that maintains the integrity of cell and controls passage of materials into and out of the cell. a. Cell Membrane c. Vacuoles b. Cell Wall d. Endoplasmic Reticulum 3. He was a Greek Philosopher, a student of Plato and teacher of Alexader the Great, also considered as the father of biology. a. Theophrastus c. Aristotle b. Matthias Schleiden d. Theodore Schwann 4. It is the functional role of a species in a community that is its occupation or how it earns its living. a. Ecosystem c. Niche b. Work d. Occupation 5. Indicates the total amount of energy present in each trophic level that shows the loss of energy from one trophic level to the next. a. Energy pyramid c. Food Pyramid b. Taxonomy d. Biomass 6. German physiologist who contributes that animal is made up of lot of cells the discovery of the organic nature of yeast and invention of the term metabolism. a. Rudolf Virchow c. Aristotle b. Matthias Schleiden d. Theodore Schwann 7. The first person who use the term cells for the tiny structures found in organisms and observe a piece of cork by the use of microscope which he himself had made. a. Rudolf Virchow c. Robert Hooke b. Matthias Schleiden d. Theodore Schwann 8. It refers to the theory about the origin of life which life originated spontaneously from non-living things. a. Marine Theory c. Divine Creation Theory b. Evolutionary Theory d. Abiogenesis Theory 9. Life originated from outer planets in a form of a resistance poor propelled by radiation pressure reach earth and started the first form of life. a. Marine Theory c. Divine Creation Theory b. Cosmozoic Theory d. Abiogenesis Theory 10. He conducted an experiment with nutrient both and curved neck flask to finally disprove spontaneous generation. a. Louis Pasteur c. Lazzaro Spallanzani b. Francesco Redi d. John Needham
5th Grade Science Quiz: Movement of Matter in Ecosystems 1. Which organisms are responsible for breaking down dead plants and animals into simple nutrients? a) Producers b) Consumers c) Decomposers d) Predators 2. What is the process by which plants use sunlight to make food? a) Respiration b) Decomposition c) Photosynthesis d) Erosion 3. How do animals contribute to the movement of matter in an ecosystem? a) They produce oxygen b) They break down dead organisms c) They release nutrients through waste d) They absorb carbon dioxide 4. What is the primary source of energy for most ecosystems? a) Soil b) Water c) Sunlight d) Air 5. How does matter move through the environment? a) It flows in a straight line b) It cycles through various organisms and the environment c) It remains stationary d) It only moves between plants and animals Answer Key (Always review AI generated answers for accuracy - Math is more likely to be inaccurate.) 1. c) Decomposers 2. c) Photosynthesis 3. c) They release nutrients through waste 4. c) Sunlight 5. b) It cycles through various organisms and the environment
Digestive System. Teeth help break down the food to small pieces. Tongue moves food to the back of the mouth and to the opening of the esophagus. Saliva is 99% water and enzymes that begin to chemical digestion. Small Intestine is a coiled tube like organ is 20feet long. This is when nutrients are taken up by the body. Villus is the spot that nutrients are pass out of the small intestine to the body. Liver is a large organ that produces bile to digest fat. Gallbladder produces bile as needed into the small intestine. Pancreas is an organ that produces enzymes and release directly into the small intestine. Colon or large intestine is an organ that absorbs most of the liquid from undigested food. Rectum is where solid waste is stored. Anus is the opening to the out side . The main function of the digestive system is to turn the food into simple sugars, amino acids, and carbohydrates. This is fuel for the human body. The first stage of the digestive system is the mouth and teeth. The teeth grid up the food. Which saliva is mix with the food to break the food down. The food is swallowed and wave like motion moves the food to the stomach. The second stage is the stomach breaks down the food. The stomach churns the food. Mixing the food with the gastric juices. This is done with the gastric juices are mix in the stomach. The glands in the stomach produce the juices. The gastric juices break down the proteins. Then the food is passed into the small intestine. In the small intestine which is about 20ft long. This is where the small intestine absorbs the nutrients from the food. Most digestion takes place in the duodenum of the small intestine. Small finger like projections called villus that collect the nutrients. These nutrients are passed into the bloodstream. The three organs that help in digesting the food. Liver, and gallbladder. Liver produces bile , a substance that aids in digestion of fats. Gallbladder holds and releases bile into the small intestine as needed. Pancreas lies across the back of the abdomen. The pancreas produces enzymes that are necessary to break down carbohydrates, proteins, and fats. Cells in the pancreas are called Islet of Langerhans, which produce two hormones (glucagon, and insulin. These regulate sugar in the blood. Insulin is a hormone that stimulates the liver to convert glucose to glycogen. Glucagon is a hormone that stimulates the liver to convert glycogen to glucose.
Kindly create a 30 items multiple choice test from this laboratory activity entitled laboratory do's and donts: LABORATORY SAFETY Dos: Wear Appropriate Attire: Wear lab coats, safety goggles, gloves, and any other required personal protective equipment (PPE) at all times in the lab. Follow Protocols: Adhere strictly to established protocols and procedures for all experiments and tasks. Label Everything: Clearly label all containers, tubes, vials, and equipment with relevant information, including date, contents, and your initials. Calibrate Instruments: Regularly calibrate and maintain all lab equipment according to manufacturer guidelines to ensure accurate measurements. Keep Workspace Organized: Maintain a clean and organized workspace to prevent contamination and ensure efficient work. Dispose of Waste Properly: Follow the correct disposal procedures for hazardous waste, sharps, and non-hazardous materials in accordance with local regulations. Use Pipette Aids: Always use pipette aids or bulb fillers to avoid mouth pipetting and potential exposure to hazardous substances. Record Observations: Keep detailed and accurate records of your experiments, observations, procedures, and results. Label Samples Clearly: Label all samples with accurate and descriptive information to avoid mix-ups and confusion. Communicate: Maintain clear communication with colleagues and supervisors about your work, findings, and any potential issues. Follow Safety Guidelines: Adhere to all safety guidelines, emergency procedures, and evacuation plans in case of accidents or incidents. Report Accidents and Incidents: Report any accidents, spills, or incidents to your supervisor immediately, no matter how minor they may seem. Don'ts: Don't Eat, Drink, or Smoke: Never consume food, drinks, or smoke inside the laboratory to prevent contamination and chemical exposure. Don't Pipette by Mouth: Avoid mouth pipetting to prevent the risk of inhaling or ingesting hazardous substances. Don't Use Chipped Glassware: Do not use chipped, cracked, or compromised glassware, as they can lead to leaks and contamination. Don't Work Alone: Avoid working in the lab alone, especially with hazardous materials or equipment. Don't Ignore Safety Procedures: Never disregard safety procedures or skip steps, even if you're experienced with a particular task. Don't Contaminate Reagents: Avoid contaminating reagents by using clean tools, pipettes, and containers. Don't Rush: Take your time and follow protocols accurately. Rushing can lead to mistakes and unsafe conditions. Don't Block Emergency Equipment: Keep emergency equipment, such as eyewash stations, fire extinguishers, and safety showers, unobstructed and easily accessible. Don't Pour Chemicals into Sinks: Do not pour chemicals down sinks unless you are certain they are safe to do so, as this can lead to environmental contamination. Don't Use Unlabeled Chemicals: Never use unlabeled or improperly labeled chemicals. Always know what you're working with. Don't Wear Loose Clothing or Jewelry: Avoid wearing loose clothing, open-toed shoes, and excessive jewelry that could get caught in equipment or chemicals. Don't Assume, Ask: If you're unsure about something, never assume. Always ask for guidance from your supervisor or colleague