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Adv. Biotech - RT-PCR and Microarrays
Quiz by Ese Ararile
Tag the questions with any skills you have. Your dashboard will track each student's mastery of each skill.
Real-Time PCR uses end-point analysis on agarose gels
Real-Time PCR is only qualitative
The lowercase "rt" in "rt RT-PCR" stands for?
What is end-point PCR?
Real-time PCR measures/detects product as the reaction progresses (in “real time”) through the generation of a _____ that is proportional to the amount of PCR product formed
In RT-PCR, the computer translates the fluorescence data into meaningful results. So what does the optical module do?
Why is the in-real-life PCR product not amplified by 2 as is expected by the theoretical yield?
What are the two major steps of gene expression?
In Eukaryotes most genes are turned off until needed, while in prokaryotes most genes are turned on.
dsDNA binding dyes and fluorescent probes are used together as reporters at the same time
The increase in fluorescent signal is inversely proportional to the number of PCR product molecules (amplicons) generated in the exponential phase.
Fluorescence only occurs when ...
Match the reporters
Rank the steps accordingly for rt-RT PCR
SYBR Green I is the most common binding dye used in RT-PCR. How does it bind to double-stranded DNA?
With bound SYBR Green I, fluorescence increases up to _____ fold.
Over fluorescent signal from SYBR Green reaction is proportional to the amount of dsDNA and ____ as the target is amplified.
What happens if you use SYBR Green in a duplex PCR?
The TagMan probe is made up of ____ and ____
With the TaqMan technique, the fluorescence is produced when?
The TaqMan probe competes with the primers for binding sites on the DNA
Challenges of rt-RT PCR
What are the limitations of using dsDNA binding dyes
Pick all TRUE about TaqMan probe
TaqMan and Taq Polymerase are the same thing
You would need unique TaqMan probes for each experiment
Real-Time PCR is _____ than conventional PCR
Using this diagram, what is the CT (Cycle threshold) for the Red sample?
If the CT is lower, the DNA content in that sample is most likely ____
What is the equation for the difference in DNA content?
What is amplified in the process of rt-RT PCR?
The rt-RT PCR process helps you pinpoint the level of ....?
Should you re-standardize your qRT-PCR enzymes after leaving it overnight?
Factors that cause errors in the rt PCR results
Microarrays must be bigger than a microscope slide
Each spot on a microarray represents one ___
A DNA microarray allows for experiments to be performed on thousands of genes at the same time.
Masks in the microarrays protect the blocker from what?
