
Adv. Biotech - SNP LAB
Quiz by Ese Ararile
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Name the 5 basic tastes (small letters)
Taste recognition is mediated by specialized taste cells that communicate with several brain regions through direct connections to _____
Taste perception is a ____ step process
Organize the tasting process
If a bitter taste receptor is expressed on the surface of a “sweet cell,” a bitter molecule is perceived as tasting what?
Inability to taste PTC is a dominant trait that varies in the human population
What molecule with the acronym "PTC" was responsible for the discovery of taste differences?
Bitter-tasting compounds are recognized by _____ on the surface of taste cells
There are approximately ____ genes for different bitter taste receptors in mammals
What gene is responsible for the PTC taste receptor?
Sequencing identified ____ nucleotide positions that vary within the human population—each variable position is termed a single nucleotide polymorphism (SNP).
One specific combination of the three SNPs, termed a _____ correlates most strongly with tasting ability
Analogous changes in other cell-surface molecules do not influence the activity of drugs.
SNPs in serotonin transporter and receptor genes predict adverse responses to anti-depression drugs, including PROZAC® and Paxil®
What is the function of Chelex resin used to boil the DNA sample?
Why is the restriction enzyme HaeIII used?
One allele is cut by the enzyme, and one is not—producing a _____ that can be separated on a 2% agarose gel
The class results show what?
Why was the saline mouth rinse centrifuged first?
Organize the steps of the experiment
Group the PCR step times accordingly
The restriction enzyme digestion was done at ____ and ____
pBR322/BstNI size markers were used as the ladder for the electrophoresis step
The gel ran at130 V for ~30 minutes. Adequate separation was achieved when cresol red dye front moved at least ____ from the wells.
Link the sequence to the tasting ability
Link the number of expected bands from the DIGESTED sample to the Taster type after electrophoresis
Homozygous genotype
What size should the Undigested PCR product be approximately?

Tt taster (heterozygous) shows three bands that represent both alleles. What bands are we expecting?
It is common to see a diffuse (fuzzy) band that runs just ahead of the 44-bp fragment. This is a _____, an artifact of the PCR reaction that results from the primers overlapping one another and amplifying themselves
The presence of primer dimer, in the absence of other bands, confirms that the reaction contained all components necessary for amplification.