General Microbiology
Quiz by Sonal Saxena
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- Q1
A 25 year old truck driver developed a painful uretheral discharge following sex with a commercial sex worker. Gram stain of discharge revealed Gram negative diplococci. The uretheral discharge has to be sent to laboratory for culture. What is the best method to transport this specimen? A 25 year old truck driver developed a painful uretheral discharge following sex with a commercial sex worker. Gram stain of discharge revealed Gram negative diplococci. The uretheral discharge has to be sent to laboratory for culture. What is the best method to transport this specimen?
Amie’s medium is used as transport medium
The discharge can be sent in universal container. The discharge can be sent in universal container.
Robertson cooked meat broth is used as transport medium
The uretheral swab can be sent directly to laboratory if the delay is not more than twenty four hours.
30s - Q2
A tertiary care hospital operates its operation theater from Monday to Saturday. Nursing sister in-charge needs to sterilize OT and prepare instruments for next week on Sunday. As medical officer in-charge of OT you need to answer her queries.Which of the following is best method for sterilization of surgical instruments?
Absolute alcohol
Household bleach
UV Radiation
Autoclave
30s - Q3
A non spore forming Gram positive bacilli was isolated from the throat specimen of a 5 year old child who has never been vaccinated. A non pathogenic strain may develop into pathogenic strain by means of a bacteriophage infection. Transfer of genetic material by a temperate bacterial virus is defined as which of the following?
Recombination
Transformation
Conjugation
Transduction
30s - Q4
A 5 year old girl presents with history of sore throat and fever. A throat swab has been collected and sent for microbiology culture. The throat swab after processing has to be discarded as per Biomedical Waste management rules, 2016. Which is the best method of discarding it?
Yellow bag
Red bag
Black bag
White bag
30s - Q5
A 60 year old woman was admitted to hospital for hysterectomy. She develops symptoms suggestive of urinary tract infection on the day of admission.This is a case of:
Healthcare associated infection
Catheter associated infection
Iatrogenic infection
Community acquired infection
30s - Q6
A medical emergency unit wants to start ZN staining for sputum specimen. The nursing sisters have made a list of chemicals required for the same. Which of the following list is correct and will serve the purpose of staining?
List 1: carbol fuschin, safranin, malachite green and 25% sulphuric acid
List 2: carbol fuschin, methylene blue and 20% sulphuric acid
List 3: carbol fuschin, methylene blue and 25% sulphuric acid
List 4: carbol fuschin, safranin, malachite green and 20% sulphuric acid
30s - Q7
A 20 year old man presents to OPD with complaints of cough and high grade fever. Sputum sample on Gram staining shows a mixture of Gram positive and Gram negative organisms. The cell wall of two types of organism is different. Which of the following statement is true regarding the correct morphology of Gram negative cell wall?
It is thinner than Gram positive cell wall
It contains mesodiaminopimelic acid
It contains teichoic acid
The lipid content of the cell wall is nil or scanty
30s - Q8
A 15 year old boy suffers a road traffic accident. After few days his wound develops crepitus. Gram stain performed on pus sample from the wound reveals Gram positive bacilli with empty refractile spaces. The report of “Sporing Gram positive bacilli” is made. Which of the following is TRUE regarding bacterial spores?
They are resistant to most of the routinely used disinfectants
They can be seen in all bacteria
These are a method of reproduction of bacteria
They have a single layered wall
30s - Q9
A 30 year old women suffers from recurrent bouts of urinary tract infections. She is prescribed an antibiotic which would control microbial nucleotide synthesis by inhibiting dihydrofolate acid reductase in bacteria Which of the following works by this mechanism?
Penicillin
Trimethoprim
Amphotericin
Chloramphenicol
30s - Q10
What is the cell wall and how it can be demonstrated?
Users enter free textType an Answer120s - Q11
Identify the staining
ZN staining
Negative staining
Gram staining
Albert staining
30s - Q12
A 40 year old male admitted in ICU develops features suggestive of sepsis. A blood culture reveals multidrug resistant Pseudomonas aeruginosa. After two days he develops acute renal failure and features of endotoxic shock. Which of the following is correct regarding the biological effect produced by endotoxin?
Endothelial activation causing increase in vascular permeability
Suppression of complement
Causes mast cell supression
Decrease in the levels of IL-1, TNF-ἀ and prostaglandins
30s - Q13
Identify the medium
TSA
TGB
RCM
SDA
30s - Q14
RCM is used for growth of anerobic organisms
truefalseTrue or False30s - Q15
Which of the following is a transport medium
Amies
Chocolate agar
Nutrient agar
VTMBlood agar
Stuart30s
