
General Microbiology
Quiz by Sonal Saxena
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A 25 year old truck driver developed a painful uretheral discharge following sex with a commercial sex worker. Gram stain of discharge revealed Gram negative diplococci. The uretheral discharge has to be sent to laboratory for culture. What is the best method to transport this specimen? A 25 year old truck driver developed a painful uretheral discharge following sex with a commercial sex worker. Gram stain of discharge revealed Gram negative diplococci. The uretheral discharge has to be sent to laboratory for culture. What is the best method to transport this specimen?Â
A tertiary care hospital operates its operation theater from Monday to Saturday. Nursing sister in-charge needs to sterilize OT and prepare instruments for next week on Sunday. As medical officer in-charge of OT you need to answer her queries.Which of the following is best method for sterilization of surgical instruments?Â
A non spore forming Gram positive bacilli was isolated from the throat specimen of a 5 year old child who has never been vaccinated. A non pathogenic strain may develop into pathogenic strain by means of a bacteriophage infection. Transfer of genetic material by a temperate bacterial virus is defined as which of the following?
A 5 year old girl presents with history of sore throat and fever. A throat swab has been collected and sent for microbiology culture. The throat swab after processing has to be discarded as per Biomedical Waste management rules, 2016. Which is the best method of discarding it?Â
A 60 year old woman was admitted to hospital for hysterectomy. She develops symptoms suggestive of urinary tract infection on the day of admission.This is a case of:
A medical emergency unit wants to start ZN staining for sputum specimen. The nursing sisters have made a list of chemicals required for the same. Which of the following list is correct and will serve the purpose of staining?Â
A 20 year old man presents to OPD with complaints of cough and high grade fever. Sputum sample on Gram staining shows a mixture of Gram positive and Gram negative organisms. The cell wall of two types of organism is different. Which of the following statement is true regarding the correct morphology of Gram negative cell wall?
A 15 year old boy suffers a road traffic accident. After few days his wound develops crepitus. Gram stain performed on pus sample from the wound reveals Gram positive bacilli with empty refractile spaces. The report of “Sporing Gram positive bacilli” is made. Which of the following is TRUE regarding bacterial spores?Â
A 30 year old women suffers from recurrent bouts of urinary tract infections. She is prescribed an antibiotic which would control microbial nucleotide synthesis by inhibiting dihydrofolate acid reductase in bacteria Which of the following works by this mechanism?
What is the cell wall and how it can be demonstrated?
Identify the staining

A 40 year old male admitted in ICU develops features suggestive of sepsis. A blood culture reveals multidrug resistant Pseudomonas aeruginosa. After two days he develops acute renal failure and features of endotoxic shock. Which of the following is correct regarding the biological effect produced by endotoxin?
Identify the medium

RCM is used for growth of anerobic organisms
Which of the following is a transport medium