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General Microbiology

Quiz by Sonal Saxena

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15 questions
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  • Q1

    A 25 year old truck driver developed a painful uretheral discharge following sex with a commercial sex worker. Gram stain of discharge revealed Gram negative diplococci. The uretheral discharge has to be sent to laboratory for culture. What is the best method to transport this specimen?  A 25 year old truck driver developed a painful uretheral discharge following sex with a commercial sex worker. Gram stain of discharge revealed Gram negative diplococci. The uretheral discharge has to be sent to laboratory for culture. What is the best method to transport this specimen? 

    Amie’s medium is used as transport medium  

    The discharge can be sent in universal container. The discharge can be sent in universal container.

    Robertson cooked meat broth is used as transport medium

    The uretheral swab can be sent directly to laboratory if the delay is not more than twenty four hours. 

    30s
  • Q2

    A tertiary care hospital operates its operation theater from Monday to Saturday. Nursing sister in-charge needs to sterilize OT and prepare instruments for next week on Sunday. As medical officer in-charge of OT you need to answer her queries.Which of the following is best method for sterilization of surgical instruments? 

    Absolute alcohol

    Household bleach

    UV Radiation 

    Autoclave

    30s
  • Q3

    A non spore forming Gram positive bacilli was isolated from the throat specimen of a 5 year old child who has never been vaccinated. A non pathogenic strain may develop into pathogenic strain by means of a bacteriophage infection. Transfer of genetic material by a temperate bacterial virus is defined as which of the following?

    Recombination

    Transformation

    Conjugation

    Transduction 

    30s
  • Q4

    A 5 year old girl presents with history of sore throat and fever. A throat swab has been collected and sent for microbiology culture. The throat swab after processing has to be discarded as per Biomedical Waste management rules, 2016. Which is the best method of discarding it? 

    Yellow bag 

    Red bag  

    Black bag 

    White bag

    30s
  • Q5

    A 60 year old woman was admitted to hospital for hysterectomy. She develops symptoms suggestive of urinary tract infection on the day of admission.This is a case of:

    Healthcare associated infection

    Catheter associated infection 

    Iatrogenic infection

    Community acquired infection 

    30s
  • Q6

    A medical emergency unit wants to start ZN staining for sputum specimen. The nursing sisters have made a list of chemicals required for the same. Which of the following list is correct and will serve the purpose of staining? 

    List 1: carbol fuschin, safranin, malachite green and 25% sulphuric acid

    List 2: carbol fuschin, methylene blue and 20% sulphuric acid

    List 3: carbol fuschin, methylene blue and 25% sulphuric acid

    List 4: carbol fuschin, safranin, malachite green and 20% sulphuric acid

    30s
  • Q7

    A 20 year old man presents to OPD with complaints of cough and high grade fever. Sputum sample on Gram staining shows a mixture of Gram positive and Gram negative organisms. The cell wall of two types of organism is different. Which of the following statement is true regarding the correct morphology of Gram negative cell wall?

    It is thinner than Gram positive cell wall

    It contains mesodiaminopimelic acid

    It contains teichoic acid

    The lipid content of the cell wall is nil or scanty

    30s
  • Q8

    A 15 year old boy suffers a road traffic accident. After few days his wound develops crepitus. Gram stain performed on pus sample from the wound reveals Gram positive bacilli with empty refractile spaces. The report of “Sporing Gram positive bacilli” is made. Which of the following is TRUE regarding bacterial spores? 

    They are resistant to most of the routinely used disinfectants

    They can be seen in all bacteria

    These are a method of reproduction of bacteria

    They have a single layered wall 

    30s
  • Q9

    A 30 year old women suffers from recurrent bouts of urinary tract infections. She is prescribed an antibiotic which would control microbial nucleotide synthesis by inhibiting dihydrofolate acid reductase in bacteria Which of the following works by this mechanism?

    Penicillin

    Trimethoprim

    Amphotericin

    Chloramphenicol

    30s
  • Q10

    What is the cell wall and how it can be demonstrated?

    Users enter free text
    Type an Answer
    120s
  • Q11

    Identify the staining

    Question Image

    ZN staining

    Negative staining

    Gram staining 

    Albert staining

    30s
  • Q12

    A 40 year old male admitted in ICU develops features suggestive of sepsis. A blood culture reveals multidrug resistant Pseudomonas aeruginosa. After two days he develops acute renal failure and features of endotoxic shock. Which of the following is correct regarding the biological effect produced by endotoxin?

    Endothelial activation causing increase in vascular permeability

    Suppression of complement

    Causes mast cell supression

    Decrease in the levels of IL-1, TNF-ἀ and prostaglandins

    30s
  • Q13

    Identify the medium

    Question Image

    TSA

    TGB

    RCM

    SDA

    30s
  • Q14

    RCM is used for growth of anerobic organisms

    true
    false
    True or False
    30s
  • Q15

    Which of the following is a transport medium

    Amies

    Chocolate agar

    Nutrient agar

    VTM

    Blood agar

    Stuart
    30s

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