At least two lineages

red staining, needle-like bodies seen in the cytoplasm of myeloblasts, and/or progranulocytes in certain leukemias

CD3 (T-cells) and CD20 (B-cells)

CD34

TdT (terminal deoxytransferase)

Acute leukemia involves immature cells

70%; mostly seen in adults

Bleeding and bruising

Fever and infections

Bone and joint pain, lymphadenopathy, and hepatosplenomegaly

Neurologic symptoms (headache, vomiting)

Acute promyelocytic leukemia

T(8;21)- CBFalpha/ETO fusion gene; inv (16)- CBFbeta/MYH11 fusion gene; t(15;17)- RARalpha/PML fusion gene; t(11q23;v)- diverse MLL fusion genes; defines leukemia

Granulocytes; disseminated intravascular coagulation; all-trans-retinoic acid (ATRA)

Alkylating agent related; without prior myelodysplastic syndrome

Down Syndrome

B-cell precursor; no auer rods and there's a lack of cytoplasmic granules

Presents more acutely; symptoms of bone marrow suppression (low counts)

Acute lymphoblastic leukemia

TdT, CD19, and CD10

Children 2-10 is GOOD; hyperploidy >50 chromosomes is GOOD; Philadelphia chromosome is BAD

Chronic lymphocytic leukemia/ small lymphocytic lymphoma

Diffuse large B-cell lymphoma (Richter transformation)

RL is due to infectious mononucleosis (EBV)

MNRL is due to pertussis

Richter Transformation

Tumor cells in bone marrow and spleen; splenomegaly and pancytopenia

Tartrate resistant acid phosphatase (TRAP), CD11c, CD25, CD103, and Annexin A1

T-cell prolymphocytic leukemia (T-PLL)

HTLV-1 (Human T-cell leukemia virus); Japan and Caribbean; clover leaf and flow cells

Sezary Syndrome; CD4+ T-cells

Molecular markers