Question 1

What component of citrate media is selective?

Accepted Answer

Citrate

Answer

Phenol red

Answer

Nothing

Answer

Bromothymol blue

Question 2

Which component of citrate media is differential?

Accepted Answer

Bromothymol blue

Answer

Citrate

Answer

More than one of these answers

Answer

None of these answers

Question 3

The purpose of citrate testing is to determine if an organism can use citrate as an electron source.

Accepted Answer

False

Answer

True

Question 4

A citrate test result with no color change but there is growth would be interpreted as ___________.

Accepted Answer

Positive (+)

Answer

Negative (-)

Answer

Inconclusive

Question 5

A citrate test result with a blue color change would be interpreted as _________________.

Accepted Answer

Positive (+)

Answer

Negative (-)

Answer

Inconclusive

Question 6

Pictured is a result for citrate, how would this be interpreted?

Accepted Answer

Positive (+)

Answer

Inconclusive

Answer

Negative (-)

Question 7

Pictured is a result for citrate, how would this be interpreted?

Accepted Answer

Negative (-)

Answer

Positive (+)

Answer

Inconclusive

Question 8

What is the pH indicator in phenol red fermentation testing?

Accepted Answer

Phenol red

Answer

Bromothymol blue

Answer

Methyl red

Answer

None of the above

Question 9

Pictured is a result for phenol red fermentation, how would this be interpreted?

Accepted Answer

A/G

Answer

Inconclusive

Answer

A/-

Answer

-/-

Question 10

Pictured is a result for phenol red fermentation, how would this be interpreted?

Accepted Answer

A/-

Answer

Inconclusive

Answer

A/G

Answer

-/-

Question 11

Pictured is a result for phenol red fermentation, how would this be interpreted?

Accepted Answer

-/-

Answer

Inconclusive

Answer

A/-

Answer

A/G

Question 12

What is the purpose of the phenol red fermentation test?

Accepted Answer

To determine organisms' ability to ferment different carbohydrates

Answer

To determine pH

Answer

To determine color change

Answer

To determine organisms' ability to produce alcohol

Question 13

What color does the media start in phenol red fermentation?

Accepted Answer

Red

Answer

Yellow

Answer

Orange

Answer

Pink

Question 14

What is the pH indicator used in MR testing?

Accepted Answer

Methyl red

Answer

Bromothymol blue

Answer

Malachite green

Answer

Phenol red

Question 15

What color does the media start in MR/VP?

Accepted Answer

Yellow/straw

Answer

Orange

Answer

Pink

Answer

Red

Question 16

What does MR test for?

Accepted Answer

Presence of an acid product from glucose fermentation

Answer

Presence of alcohol/acetoin from glucose fermentation

Answer

None of these answers

Answer

Presence of methyl red from glucose fermentation

Question 17

What does VP test for?

Accepted Answer

Presence of alcohol/acetoin from glucose fermentation

Answer

Presence of methyl red from glucose fermentation

Answer

None of these answers

Answer

Presence of acid from glucose fermentation

Question 18

How would you interpret a copper/orange color VP?

Accepted Answer

Negative (-)

Answer

Positive (+)

Answer

Inconclusive

Question 19

How would you interpret this VP result?

Accepted Answer

Positive (+)

Answer

Inconclusive

Answer

Negative (-)

Question 20

How would you interpret this MR result?

Accepted Answer

Positive (+)

Answer

Inconclusive

Answer

Negative (-)

Question 21

How would you interpret this MR result?

Accepted Answer

Negative (-)

Answer

Positive (+)

Answer

Inconclusive

Question 22

Can an organism be both MR/VP negative?

Accepted Answer

Yes

Answer

Maybe

Answer

No

Answer

Always

Question 23

What enzyme are we testing for in starch hydrolysis?

Accepted Answer

Amylase/alpha-amylase

Answer

Urease

Answer

Catalase

Answer

Starchase

Question 24

How would you interpret this starch hydrolysis result?

Accepted Answer

Positive (+)

Answer

Inconclusive

Answer

Negative (-)

Question 25

How would you interpret this starch hydrolysis result for S. agalactiae (left)?

Accepted Answer

Negative (-)

Answer

Inconclusive

Answer

Positive (+)

Question 26

Where does the dark color come from in this starch hydrolysis come from?

Accepted Answer

Iodine

Answer

Malachite green

Answer

Methyl red

Answer

Bromothymol blue

Question 27

Should we read starch hydrolysis results on  a dark or light background?

Accepted Answer

Light

Answer

Dark

Question 28

What is another name for catalase?

Accepted Answer

Hydrogen peroxidase

Answer

Peroxidase

Answer

Oxidase

Answer

Hydrogendase

Question 29

What does catalase breakdown?

Accepted Answer

Hydrogen peroxide

Answer

Water

Answer

Gas

Answer

Oxygen

Question 30

How would you interpret this catalase test result?

Accepted Answer

Positive (+)

Answer

Inconclusive

Answer

Negative (-)

Question 31

How would you interpret this catalase test result?

Accepted Answer

Negative (-)

Answer

Positive (+)

Answer

Inconclusive

Question 32

How would you interpret this oxidase test?

Accepted Answer

Positive (+)

Answer

Negative (-)

Answer

Inconclusive

Question 33

Pictured is an oxidase result that sat for an hour, how would we interpret this?

Accepted Answer

Inconclusive, retest required

Answer

Negative (-)

Answer

Positive (+)

Question 34

How would you interpret this oxidase test?

Accepted Answer

Negative (-)

Answer

Inconclusive

Answer

Positive (+)

Question 35

What enzyme is being tested for in oxidase testing?

Accepted Answer

Cytochrome-c oxidase/oxidase

Answer

Catalase

Answer

Photodase

Answer

Amylase

Question 36

What is NO3?

Accepted Answer

Nitrate

Answer

Nitrite

Question 37

What is NO2?

Accepted Answer

Nitrite

Answer

Nitrate

Question 38

In nitrate reduction, we are testing to see if an organism can reduce NO3 into what?

Accepted Answer

NO2

Answer

NO

Answer

N2

Question 39

For nitrate reduction, the test tube on the left would be considered ______________. The test tube on the right would be considered ______________.

Accepted Answer

Positive (+), inconclusive

Answer

Positive (+), negative (-)

Answer

Negative (-), positive (+)

Answer

Negative (-), inconclusive

Question 40

What do we add, and to which tube, if these results are for nitrate reduction?

Accepted Answer

Zinc, right tube

Answer

Zinc, both tubes

Answer

Nothing, neither tube

Answer

Zinc, left tube

Question 41

For denitrification, the test tube on the left would be considered _____________. The test tube on the right would be considered ______________.

Accepted Answer

Negative (-), positive (+)

Answer

Positive (+), inconclusive

Answer

Negative (-), inconclusive

Answer

Positive (+), negative (-)

Question 42

What reaction does zinc catalyze in nitrate reduction testing?

Accepted Answer

NO3 + Zn -> NO2

Answer

NO3 + Zn -> NO

Answer

NO2 + Zn -> NO3

Answer

No reaction

Question 43

What do nitrate reduction reagents interact with in nitrate reduction testing to produce a red color?

Accepted Answer

NO2

Answer

Zn

Answer

NO3

Answer

N2

Question 44

What is the pH indicator in  urea hydrolysis?

Accepted Answer

Phenol red

Answer

Methyl red

Answer

Bromothymol blue

Answer

Pink dye

Question 45

How would you interpret results for this urea hydrolysis test?

Accepted Answer

Positive (+)

Answer

Negative (-)

Answer

Inconclusive

Question 46

How would you interpret results for this urea hydrolysis test?

Accepted Answer

Negative (-)

Answer

Positive (+)

Question 47

What enzyme is being tested for in urea hydrolysis?

Accepted Answer

Urease

Answer

Amylase

Answer

Urinase

Answer

Hydrolyase

Question 48

What is casein? Where does it come from?

Accepted Answer

A protein, milk

Answer

An enzyme, bile

Answer

An enzyme, milk

Answer

A protein, bile

Question 49

How would you interpret results for this casein hydrolysis test?

Accepted Answer

Positive (+)

Answer

Inconclusive

Answer

Negative (-)

Question 50

How would you interpret results for this casein hydrolysis test?

Accepted Answer

Negative (-)

Answer

Positive (+)

Answer

Inconclusive

Question 51

Should we interpret casein hydrolysis tests on a light or dark background?

Accepted Answer

Dark

Answer

Light

Question 52

How does the media begin in gelatin hydrolysis?

Accepted Answer

Solid

Answer

Liquid

Question 53

How would you interpret results for this gelatin hydrolysis test (media is liquid)?

Accepted Answer

Positive (+)

Answer

Negative (-)

Answer

Inconclusive

Question 54

How would you interpret results for this gelatin hydrolysis test (media is solid)?

Accepted Answer

Negative (-)

Answer

Inconclusive

Answer

Positive (+)

Question 55

What does S stand for in SIM?

Accepted Answer

Sulfur

Answer

Size

Answer

Shape

Answer

Slime

Question 56

What does I stand for in SIM?

Accepted Answer

Indole

Answer

Intensity

Answer

Irregularity

Question 57

What does M stand for in SIM?

Accepted Answer

Motility

Answer

Morphology

Answer

Make-up

Question 58

How would you interpret results for this SIM test?

Accepted Answer

S: (-), I: (-), M: (-)

Answer

S: (+), I: (-), M: (-)

Answer

S: (-), I: (+), M: (+)

Answer

S: (-), I: (-), M: (+)

Question 59

How would you interpret results for this SIM test?

Accepted Answer

S: (+), I: (-), M: (+)

Answer

S: (-), I: (-), M: (+)

Answer

S: (+), I: (+), M: (+)

Answer

S: (-), I: (-), M: (+)

Question 60

What do organisms produce that creates a black color in SIM?

Accepted Answer

H2S

Answer

SO4

Answer

Nothing

Answer

S

Question 61

What does H2S interact within the media to create a black color?

Accepted Answer

Iron

Answer

Sodium

Answer

Methane

Answer

Cholorine

Question 62

If an organism is susceptible to an antibiotic-infused Kirby-Bauer disk, what do we visualize?

Accepted Answer

Zone of inhibition

Answer

Growth

Answer

Zone of stoppage

Answer

Halted zone

Question 63

What do we use to determine if an organism is susceptible to an antibiotic?

Accepted Answer

Kirby-Bauer disks

Answer

Bauer-Kirby disks

Answer

Antibiotics disks

Answer

Kendall-Banner disks

Question 64

What enzyme does the indole portion of SIM test for?

Accepted Answer

Tryptophanase

Answer

Catalase

Answer

Indolase

Answer

Oxidase

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