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minione PCR System and minione Electrophoresis System

Quiz by Zhao

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22 questions
Show answers
  • Q1
    How do you properly prepare the Minione PCR System for use?
    Leave the system without cleaning after each use.
    Clean and disinfect the system according to the manufacturer's instructions.
    Run the system without cleaning it.
    Use any cleaning solution.
    30s
  • Q2
    What is the first step in setting up a PCR reaction on the Minione PCR System?
    Preheat the thermal cycler.
    Load the samples into the system.
    Prepare the reaction mix.
    Turn on the system.
    30s
  • Q3
    How do you properly load samples into the Minione Electrophoresis System?
    Load the samples into the buffer tank.
    Load the samples into the wells of the gel according to the manufacturer's instructions.
    Load the samples into the power supply.
    Load the samples into the UV transilluminator.
    30s
  • Q4
    How do you run an electrophoresis gel on the Minione Electrophoresis System?
    Follow the manufacturer's instructions for setting up and running the gel.
    Load the samples into the gel before it is set up.
    Start the system without preparing the gel.
    Add the samples to the buffer tank instead of the gel.
    30s
  • Q5
    How do you properly stain and visualize the electrophoresis gel results on the Minione Electrophoresis System?
    Visualize the gel without staining it.
    Follow the manufacturer's instructions for staining and visualizing the gel.
    Use any staining solution.
    Leave the gel unstained.
    30s
  • Q6
    What is the first step in cleaning and maintaining the Minione PCR System?
    Turn off the system and unplug it.
    Clean the system while it is still turned on.
    Leave the system without cleaning after each use.
    Use any cleaning solution.
    30s
  • Q7
    How do you troubleshoot common issues with the Minione PCR System?
    Refer to the manufacturer's troubleshooting guide and/or contact technical support.
    Use any solution to fix the issue.
    Ignore the issue.
    Try to fix the issue without consulting the troubleshooting guide.
    30s
  • Q8
    What is the role of Taq polymerase in the Minione PCR reaction?
    Taq polymerase modifies the target DNA.
    Taq polymerase degrades the target DNA.
    Taq polymerase measures the target DNA.
    Taq polymerase amplifies the target DNA.
    30s
  • Q9
    How do you optimize the conditions for your PCR reaction on the Minione PCR System?
    Use any conditions without optimization.
    Use the default conditions without optimization.
    Ignore optimization.
    Experiment with different conditions and parameters to find the optimal conditions for your reaction.
    30s
  • Q10
    How do you store and transport samples for use with the Minione PCR and Electrophoresis Systems?
    Ignore storage and transport.
    Use any storage and transport solution.
    Follow the manufacturer's instructions for storing and transporting samples.
    Store and transport the samples without following any instructions.
    30s
  • Q11
    How long should you denature the DNA for the initial step of the PCR reaction on the Minione PCR System?
    Denature the DNA for a longer time.
    Denature the DNA for any time.
    Follow the manufacturer's instructions for denaturation time.
    Denature the DNA for a shorter time.
    30s
  • Q12
    What is the temperature for the annealing step of the PCR reaction on the Minione PCR System?
    Use any annealing temperature.
    Use a higher annealing temperature.
    Follow the manufacturer's instructions for the annealing temperature.
    Use a lower annealing temperature.
    30s
  • Q13
    How long should the extension step of the PCR reaction last on the Minione PCR System?
    Extend the reaction for a shorter time.
    Extend the reaction for any time.
    Follow the manufacturer's instructions for extension time.
    Extend the reaction for a longer time.
    30s
  • Q14
    What is the temperature for the final extension step of the PCR reaction on the Minione PCR System?
    Use any final extension temperature.
    Follow the manufacturer's instructions for the final extension temperature.
    Use a higher final extension temperature.
    Use a lower final extension temperature.
    30s
  • Q15
    How many cycles should the PCR reaction run on the Minione PCR System?
    Run fewer cycles than recommended.
    Follow the manufacturer's instructions for the number of cycles.
    Run any number of cycles.
    Run more cycles than recommended.
    30s

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