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PCR& Gel electrophoresis & DNA sequencing

Quiz by Zhao

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40 questions
Show answers
  • Q1
    What is the purpose of gel electrophoresis?
    To separate DNA fragments based on their concentration
    To separate DNA fragments based on their fluorescence
    To separate DNA fragments based on their charge
    To separate DNA fragments based on their size
    30s
  • Q2
    What is the source of the negative charge in DNA fragments?
    The nitrogenous bases
    The hydrogen bonds
    The secondary structure
    The sugar-phosphate backbone
    30s
  • Q3
    What type of gel is typically used for DNA separation?
    Polyacrylamide
    Silica
    Cellulose
    Agarose
    30s
  • Q4
    What is the role of the DNA ladder in gel electrophoresis?
    It helps to keep the gel in the electrophoresis chamber
    It binds to the DNA fragments to make them visible
    It serves as a standard reference for determining the size of DNA fragments
    It helps to conduct electricity through the gel
    30s
  • Q5
    Where are the DNA samples loaded in the gel electrophoresis procedure?
    On the surface of the gel
    At the positive electrode end of the gel
    In the middle of the gel
    At the negative electrode end of the gel
    30s
  • Q6
    Which electrode do the DNA fragments migrate towards during electrophoresis?
    It depends on the gel type
    The gel does not move
    The positive electrode
    The negative electrode
    30s
  • Q7
    How is the gel typically visualized after electrophoresis?
    By X-rays
    By radioactive isotopes
    By UV light
    By infrared radiation
    30s
  • Q8
    What is the advantage of capillary electrophoresis over gel electrophoresis?
    It is faster
    It can separate smaller fragments
    It is more precise
    All of the above (It is more precise, can separate smaller fragments, faster)
    30s
  • Q9
    How are the DNA fragments separated in Size Exclusion Chromatography?
    By the fluorescent properties
    Using an Electric current
    By the size of the fragments passing through porous beads
    Based on the charge of DNA fragments
    30s
  • Q10
    How many replicates of your samples should be run in gel electrophoresis?
    It depends on the experiment
    Multiple replicates
    2-3
    Only one
    30s
  • Q11
    What is the main function of the buffer in gel electrophoresis?
    To keep the gel hydrated
    To conduct electricity through the gel
    To dissolve the DNA fragments
    To cool the gel
    30s
  • Q12
    What is the typical voltage range used for running agarose gel electrophoresis?
    300-500 V
    500-1000 V
    100-300 V
    50-100 V
    30s
  • Q13
    What is the staining substance that binds to the DNA and make them visible in gel electrophoresis?
    Ethidium bromide or SYBR Green
    Hematoxylin
    Fluorescein
    Crystal violet
    30s
  • Q14
    What is the principle of separation in gel electrophoresis?
    DNA fragments move through gel based on their size and charge
    DNA fragments move through gel based on their charge only
    DNA fragments move through gel based on their mass
    DNA fragments move through gel based on their size only
    30s
  • Q15
    What is the name of the pocket-like indentations in the gel where the DNA samples are loaded?
    Grooves
    Slots
    Pits
    Wells
    30s

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