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PCR and Gel electrophoresis

Quiz by Zhao

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26 questions
Show answers
  • Q1
    What is the purpose of gel electrophoresis?
    To separate DNA fragments based on their concentration
    To separate DNA fragments based on their charge
    To separate DNA fragments based on their fluorescence
    To separate DNA fragments based on their size
    30s
  • Q2
    What is the source of the negative charge in DNA fragments?
    The secondary structure
    The nitrogenous bases
    The hydrogen bonds
    The sugar-phosphate backbone
    30s
  • Q3
    What type of gel is typically used for DNA separation?
    Cellulose
    Agarose
    Polyacrylamide
    Silica
    30s
  • Q4
    What is the role of the DNA ladder in gel electrophoresis?
    It serves as a standard reference for determining the size of DNA fragments
    It binds to the DNA fragments to make them visible
    It helps to conduct electricity through the gel
    It helps to keep the gel in the electrophoresis chamber
    30s
  • Q5
    Where are the DNA samples loaded in the gel electrophoresis procedure?
    On the surface of the gel
    In the middle of the gel
    At the positive electrode end of the gel
    At the negative electrode end of the gel
    30s
  • Q6
    Which electrode do the DNA fragments migrate towards during electrophoresis?
    The negative electrode
    The positive electrode
    The gel does not move
    It depends on the gel type
    30s
  • Q7
    How is the gel typically visualized after electrophoresis?
    By X-rays
    By infrared radiation
    By radioactive isotopes
    By UV light
    30s
  • Q8
    How are the DNA fragments separated in Size Exclusion Chromatography?
    By the size of the fragments passing through porous beads
    By the fluorescent properties
    Using an Electric current
    Based on the charge of DNA fragments
    30s
  • Q9
    How many replicates of your samples should be run in gel electrophoresis?
    Only one
    Multiple replicates
    2-3
    It depends on the experiment
    30s
  • Q10
    What is the main function of the buffer in gel electrophoresis?
    To dissolve the DNA fragments
    To conduct electricity through the gel
    To cool the gel
    To keep the gel hydrated
    30s
  • Q11
    What is the staining substance that binds to the DNA and make them visible in gel electrophoresis?
    Fluorescein
    Crystal violet
    Ethidium bromide or SYBR Green
    Hematoxylin
    30s
  • Q12
    What is the principle of separation in gel electrophoresis?
    DNA fragments move through gel based on their size and charge
    DNA fragments move through gel based on their size only
    DNA fragments move through gel based on their charge only
    DNA fragments move through gel based on their mass
    30s
  • Q13
    What is the name of the pocket-like indentations in the gel where the DNA samples are loaded?
    Grooves
    Wells
    Pits
    Slots
    30s
  • Q14
    What is the name of the substance that makes up the gel matrix?
    Agarose
    Silica
    Cellulose
    Polyacrylamide
    30s
  • Q15
    In gel electrophoresis, the DNA fragments that migrate farthest from the well towards the positive electrode are?
    The fragments with the most fluorescence
    The largest fragments
    The smallest fragments
    The fragments of the highest charge
    30s

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