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Termination of Prokaryotic DNA Replication Termination occurs near the center of a 270 bp region called ter or T locus. ter is 180O opposite of OriC and contains a sequence-specific binding protein called Tus (termination utilization substance). The GTGTGTTGT sequence that Tus binds to is repeated 5 times. The Tus protein has antihelicase activity and this ensures that replication of the genome is completed only once. At the end of prokaryotic replication the 2 new genomes are intertwined around each other but are separated by the action of topoisomerase II. | Quizalize