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Termination of Prokaryotic DNA Replication Termination occurs near the center of a 270 bp region called ter or T locus. ter is 180O opposite of OriC and contains a sequence-specific binding protein called Tus (termination utilization substance). The GTGTGTTGT sequence that Tus binds to is repeated 5 times. The Tus protein has antihelicase activity and this ensures that replication of the genome is completed only once. At the end of prokaryotic replication the 2 new genomes are intertwined around each other but are separated by the action of topoisomerase II.

Quiz by Barbara Caiquo

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8 questions
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  • Q1
    What is the function of the Tus protein in prokaryotic DNA replication termination?
    The Tus protein initiates DNA replication.
    The Tus protein repairs DNA damage.
    The Tus protein prevents overreplication by binding to a specific sequence in the ter region.
    The Tus protein promotes the separation of the two newly replicated genomes.
    The Tus protein helps stabilize the replication fork.
    30s
  • Q2
    Where does termination of prokaryotic DNA replication occur?
    Termination occurs at the origin of replication (OriC).
    Termination occurs near the center of a 270 bp region called ter or T locus.
    Termination occurs at the promoter region.
    Termination occurs at the ends of the DNA molecule.
    Termination occurs randomly throughout the genome.
    30s
  • Q3
    What is the role of the Tus protein in prokaryotic DNA replication termination?
    The Tus protein helps stabilize the replication fork.
    The Tus protein repairs DNA damage.
    The Tus protein acts as a barricade to prevent the replication machinery from advancing further.
    The Tus protein initiates DNA replication.
    The Tus protein promotes the separation of the two newly replicated genomes.
    30s
  • Q4
    What is the sequence that the Tus protein binds to in prokaryotic DNA replication termination?
    The Tus protein binds to the sequence TATAATATAT.
    The Tus protein binds to the sequence GTGTGTTGT.
    The Tus protein binds to the sequence GCGCGCGCGC.
    The Tus protein binds to the sequence AACCGGTTCC.
    The Tus protein binds to the sequence AGCTAGCTAG.
    30s
  • Q5
    What is the function of topoisomerase II in prokaryotic DNA replication termination?
    Topoisomerase II prevents overreplication.
    Topoisomerase II helps stabilize the replication fork.
    Topoisomerase II repairs DNA damage.
    Topoisomerase II initiates DNA replication.
    Topoisomerase II separates the intertwined genomes at the end of replication.
    30s
  • Q6
    What is the purpose of the ter or T locus in prokaryotic DNA replication termination?
    The ter or T locus is the site where DNA transcription occurs.
    The ter or T locus is the site where termination of DNA replication occurs.
    The ter or T locus is the site where DNA repair occurs.
    The ter or T locus is the site where DNA recombination occurs.
    The ter or T locus is the site where DNA replication begins.
    30s
  • Q7
    What is the role of antihelicase activity in prokaryotic DNA replication termination?
    Antihelicase activity of the Tus protein repairs DNA damage.
    Antihelicase activity of the Tus protein stabilizes the replication fork.
    Antihelicase activity of the Tus protein promotes DNA unwinding by helicase.
    Antihelicase activity of the Tus protein prevents the unwinding of DNA by helicase.
    Antihelicase activity of the Tus protein initiates DNA replication.
    30s
  • Q8
    What ensures that replication of the prokaryotic genome is completed only once?
    The presence of the ter or T locus ensures that replication is completed only once.
    The activity of DNA polymerase ensures that replication is completed only once.
    The binding of helicase ensures that replication is completed only once.
    The binding of topoisomerase II ensures that replication is completed only once.
    The antihelicase activity of the Tus protein ensures that replication is completed only once.
    30s

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