
Chem Midterm 1
Quiz by Lauren Phoenix
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_____ is an energy transfer from fluorophore to rhodopsin
______ store information, the genetic code of the cell
a OH group on nucleic acid means its ____ and H is ____
If NH3 is closest to the carboxylic acid it is an _____ amino acid
what is the role of the promoter
_______ is when enzymes change the structure of amino acids
_______ is more prone to hydrolysis so can adopt _____ secondary structures and is ____ stable
____ is the process of an acid forming a base
a strong acid has a ____ Ka, so ____ pka
A/T forms ____ H bonds and G/C forms ____
a ______ differs by placement of 1 H
DNA absorbs UV light more easily in _____ strand
lower enthalpy results in _____ stable
why are G/C more stable?
at ____ temp the left side (duplex) is favoured and delta G is greater than 0
at ____ temp when T is large, delta S dominates
DNA can hybridize with any part of strand as long as Tm is _____ than Temp
____ are molecules that insert into DNA duplex
Which is not a protein-DNA interaction
_____ base pairing binds parallel to purine strand
what is the benefit to solid phase synthesis
why do we use base to deprotect DNA
DMT is removed using ____
how are molecules seperated when run through a solid porous matrix
what is the driving factor in high pressure liquid chromatography
in biological DNA replication enzymes attach on ____' end and in chemical ____' end
_____ chops off terminal nucleobases when not paired properly
____ and _____ help unwind DNA
____ starts the pairing sequence before primer binds to finish off
______ puts okazaki fragments together
In biological synthesis, nucleophile is 3' _____ and electrophile is 5' ______
_____ get shorter in each replication until eventually they're too short and cell dies
in elctrophoresis longer strands move _____
on agarose gel DNA runs _____ to ______
agarose gel seperates _____ strands and polyacrilamide seperates _______ strands
______ doesn't need primase but needs an enzyme that survives at high temperature
in PCR, every thermal cycle _____ the sequence
in epigenetics, bacteria will methylate _______ to make 5-methyl ______ to distinguish it from foreign DNA
if sample sequence gets read as a C then it was a ______
DNA sticky ends are complementary to ______ sticky ends
non methylated DNA will get chopped up which prevents ______
restriction endonucleases require a ______
_______ breaks up methylated DNA
what can be used instead of Mg, though copies less carefully
random mutations are likely to destroy protein function because
what are the 3 types of cuts that ligase can form covalent bonds between O/P
Is ATP a catalyst in adenylationÂ
_____ is more stable than double strand DNA
a ______ is a duplex between 2 stem loop structures
transcription factors can _____ or ______ a gene
B-galactosidase digests lactose and makes allolactose only when
CAP will bind to promoter and recruit RNA polymerase only when_______ because then glucose is low
if bait and prey domains connect _________ occurs
transcription is more complex in _____ than ______
In eukaryotes, the 5' end has a _____ and 3' has ______
In SAM the leaving group is
in mammalian mRNA, spliceosome removes ______
the ______ links phosphate to 5' end of G
an ____nuclease cleaves in the middle and an _____nuclease cleaves at the end