
Environmental Microbiology - Mudwatt Lab
Quiz by Ese Ararile
Tag the questions with any skills you have. Your dashboard will track each student's mastery of each skill.
Link the set-up correctly
The graphite disc with the anode goes in first
You insert the red/orange wire (cathode) next to the minus sign and the green wire (anode) next to the plus sign.
Link the setup for the hacker board correctly. Remember that pin 3 and 4 remain empty.
An ____ is an organism that produces complex organic compounds from simple substances present in its surroundings, generally using energy from light (photosynthesis) or inorganic chemical reactions (chemosynthesis).
Types of autotrophs
Phototrophs use light as ____ source
Chemotrophs use electron donors from only inorganic sources as a source of energy
A heterotroph is an organism that uses pre-made organic carbon as food. If the heterotroph uses chemical energy, it is a chemoheterotroph; if it uses light for energy, it is a ____.
In aerobic respiration, oxygen (O2) is the terminal electron acceptor. In anaerobic respiration, other electron acceptors are used. Pick all that apply
Electrogenic bacteria are organisms that can transfer electrons to _____ during respiration, thus capable of generating electricity.
Pick all known electrogenic bacteria listed
In general, environmental bacteria of the genus Geobacter and Shewanella are considered promising electron generators
Microbial fuel cell (MFC) is a bioreactor that converts chemical energy present in the organic substrates to electrical energy through catalytic reactions of ____ or facultatively anaerobic microorganisms
Pick the best substrates that can be used to feed the microbial fuel cell
Microbial fuel cells work because anaerobic bacteria create a thin film on an electrode buried in soil or sediment. These electrogenic bacteria deposit their electrons onto the ____ material (graphite fibre)
Electrons flow through the wire from the anode to the cathode, where electrons combine with oxygen and hydrogen near the cathode to form water.
It is the circulation of the electrons from the anode to the cathode back to the soil that generates electrical current due to formation of a complete external circuit
Pick all items used in the lab experiment (by all)
The Anode and cathode are made of conductive graphite fibres, which can cause electrical short circuits. To prevent any damage, keep the conductive fibers away from other electronics and wear the included gloves during construction. These are
What are the electron transfer methods used by the microbes? Link the correct definitions.
Link the MudWatt components to their descriptions
This is the Cathodic reaction: CH3COO- + 2H2O ⟶ 2CO2 + 7H+ + 8e-
This is the Cathodic reaction: O2 + 4e− + 4H+ → 2H2O
Complete this Anodic rxn: _____ + 2H2O -> 2CO2 + 7H+ + 8e-
Complete the Cathodic rxn: O2 + ___ + 4H+ → 2H2O
Link the Cathode reaction correctly
Group the Anodic Reaction elements appropriately
At the anode, oxygen from the air is used to make water by combining with hydrogen from the soil digestion by the bacteria.