
Gel electrophoresis
Quiz by Jamie Moon
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10 questions
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- Q1Which direction do DNA fragments move during gel electrophoresis?Toward the positive electrodeToward the negative electrodeIn a random directionThey don't move30s
- Q2Which type of gel is commonly used in gel electrophoresis?Polyacrylamide gelAgarose gelSodium chloride gelDNA gel30s
- Q3What is gel electrophoresis used for?Separating and analyzing DNA fragmentsCreating DNA moleculesAmplifying DNA fragmentsStoring DNA samples30s
- Q4Which electrode is the DNA typically loaded onto during gel electrophoresis?No electrodePositive electrodeBoth positive and negative electrodesNegative electrode30s
- Q5What does PCR stand for?Protein Chain ReactionPolymeric Coating ReactionPolymerase Chain ReactionPurine-Contains Reaction30s
- Q6What is the purpose of the denaturation step in a PCR reaction?To amplify the DNATo add primersTo cool down the reactionTo separate the DNA strands30s
- Q7What is the role of primers in a PCR reaction?To cool down the reactionTo initiate DNA synthesisTo separate the DNA strandsTo amplify the DNA30s
- Q8What is the ideal temperature for annealing in a PCR reaction?Room temperatureBetween 50°C and 65°CAbove 70°CBelow 40°C30s
- Q9What is the purpose of the extension step in a PCR reaction?To separate the DNA strandsTo synthesize new DNA strandsTo amplify the DNATo add primers30s
- Q10Which enzyme is commonly used in PCR?RNA polymeraseTaq polymeraseReverse transcriptaseDNA ligase30s