1. Which day involves a restriction digest in the lab procedure?

2. What is the final incubation temperature set for after a PCR reaction?

3. What is the function of a loading dye in gel electrophoresis?

4. At what temperature is the restriction digest for PTC genotyping conducted?

5. How long is the incubation period for the restriction digest in the PTC tasting lab?

6. What is the purpose of the HaeIII restriction enzyme in the PTC taste lab?

7. What type of gel is used in the electrophoresis for PTC tasting?

8. On which day do students perform gel electrophoresis of PCR products?

9. How many base pairs long is the TAS2R38 gene fragment amplified for the lab?

10. Which reagent is added directly to PCR tubes to initiate the restriction digest?

11. What does the presence of three bands on a gel indicate about the TAS2R38 genotype after a restriction digest?

12. Which PCR product size is associated with non-tasters?

13. How is DNA visualized during gel electrophoresis in the PTC lab?

14. What percentage of the study participants were found to have the non-taster SNP combination of AVI?

15. What day involves DNA extraction from cheek cells?

16. Which device is used to monitor the progress of PCR in real-time?

17. What role does the MiniOne® Electrophoresis System play in the lab?

18. Which material is provided for students to taste PTC during the lab?

19. What day includes setting up PCR reactions by students?

20. What is the role of the TBE running buffer in gel electrophoresis?

21. On which day do students prepare and run their PCR amplification?

22. What is added to the PCR tube to break open cells and release DNA?

23. During which day is the restriction enzyme diluted immediately before use?

24. What method is described for ensuring PCR reagents reach the bottom of the tube?

25. Which type of micropipette is recommended for pipetting small volumes in the lab?

26. What genetic concept is illustrated by the segregation of PTC tasting alleles in a family?

27. How are PCR products differentiated by size during gel electrophoresis?

28. What is the primary purpose of the MiniOne® PCR mobile app in the PTC tasting lab?

29. What indicates a successful restriction digest in the PTC tasting lab?

30. What safety equipment is necessary for students during the lab sessions?

31. Which tube is labeled for storing the DNA extraction solution?

32. What outcome suggests the need for troubleshooting in gel electrophoresis?

33. What is indicated by a single band in the PTC tasting restriction digest?

34. What is a primary reason for the failure of a PCR reaction to produce expected results?

35. What temperature is used for the initial denaturation step in PCR for the TAS2R38 gene?

36. Which type of gel is suitable for resolving small DNA fragments such as those produced in the PTC genotyping lab?

37. What can cause faint or no bands to appear on an electrophoresis gel?

38. How is the TAS2R38 allele that allows tasting of PTC identified in the lab?

39. What is required to ensure accurate pipetting during the lab procedures?

40. Why is it necessary to balance the centrifuge before use?