Question 1

What is the required incubation temperature for the restriction digest?

Accepted Answer

37°C

Answer

94°C

Answer

25°C

Answer

4°C

Question 2

How long should the restriction digest be incubated at the constant temperature?

Accepted Answer

15 minutes

Answer

30 minutes

Answer

5 minutes

Answer

20 seconds

Question 3

What is the final incubation temperature setting for the PCR system after the digest?

Accepted Answer

4°C

Answer

0°C

Answer

25°C

Answer

37°C

Question 4

Which side of the comb should be used when casting the MiniOne agarose gel?

Accepted Answer

9-well side

Answer

Flat side

Answer

6-well side

Answer

12-well side

Question 5

How long does the gel typically take to solidify in the casting system?

Accepted Answer

10-15 minutes

Answer

30-45 minutes

Answer

20-25 minutes

Answer

2-5 minutes

Question 6

What volume of Loading Dye (LD) should be added to each "D" or "U" tube?

Accepted Answer

3 µL

Answer

5 µL

Answer

1 µL

Answer

10 µL

Question 7

After adding Loading Dye and capping the tubes, what action should be taken to mix?

Accepted Answer

Flick the tubes

Answer

Stir with a pipette tip

Answer

Heat to 37°C

Answer

Vortex for 1 minute

Question 8

What should be done to the gel tray before placing it into the electrophoresis tank?

Accepted Answer

Wipe off excess agarose from the bottom

Answer

Wash with soap

Answer

Soak in TBE buffer for 5 minutes

Answer

Freeze it for 2 minutes

Question 9

What is the total volume of TBE running buffer used in the MiniOne tank?

Accepted Answer

135 mL

Answer

50 mL

Answer

200 mL

Answer

100 mL

Question 10

Why is the buffer poured into one side first to push air out from under the tray?

Accepted Answer

To ensure clear imaging without bubbles

Answer

To prevent the gel from melting

Answer

To increase the voltage

Answer

To keep the gel at 4°C

Question 11

What volume of the MiniOne DNA marker should be loaded into the well?

Accepted Answer

10 µL

Answer

3 µL

Answer

15 µL

Answer

5 µL

Question 12

For how many minutes should the MiniOne Electrophoresis System run?

Accepted Answer

25 minutes

Answer

10 minutes

Answer

15 minutes

Answer

60 minutes

Question 13

What tool is used to document the final results of the gel?

Accepted Answer

A phone or camera

Answer

A pH meter

Answer

A microscope

Answer

A spectrophotometer

Question 14

What is the agarose concentration of the gel prepared in this lab?

Accepted Answer

0.02

Answer

0.01

Answer

0.008

Answer

0.04

Question 15

What is the purpose of the 4°C final incubation setting?

Accepted Answer

To stop the enzyme reaction

Answer

To evaporate the Loading Dye

Answer

To melt the agarose

Answer

To activate the DNA marker

Question 16

What are the specific sizes of the bands in the MiniOne Molecular Weight Marker?

Accepted Answer

100, 300, 500, 1000, 2000 bp

Answer

200, 400, 600, 800 bp

Answer

50, 100, 150 bp

Answer

1000, 2000, 3000 bp

Question 17

Which gene is being analyzed for genotypes in this experiment?

Accepted Answer

TAS2R38

Answer

BRCA1

Answer

HBA1

Answer

ACE2

Question 18

If a child inherits a different set of SNPs than those on their mother's chromosomes, what likely occurred?

Accepted Answer

Genetic recombination

Answer

Mitosis

Answer

DNA replication

Answer

Translation

Question 19

What is the probability of a taster child if one parent is homozygous non-taster and one is heterozygous?

Accepted Answer

0.5

Answer

0.75

Answer

0.25

Answer

1

Question 20

In Mendelian genetics, can two non-taster parents have a taster child?

Accepted Answer

No

Answer

Only in females

Answer

Yes

Answer

Only if they are heterozygous

Question 21

What is a potential non-genetic reason for differences in PTC tasting ability?

Accepted Answer

Age or dietary habits

Answer

Height

Answer

Blood type

Answer

Eye color

Question 22

Why is it important to ensure the centrifuge is balanced?

Accepted Answer

To prevent damage and ensure proper spinning

Answer

To separate DNA by weight

Answer

To keep the tubes warm

Answer

To mix the Loading Dye

Question 23

How are DNA fragments separated during gel electrophoresis?

Accepted Answer

By size

Answer

By color

Answer

By temperature

Answer

By magnetic pull

Question 24

What is used to visualize the DNA bands during and after the run?

Accepted Answer

Blue light

Answer

Green LED

Answer

White light

Answer

Infrared light

Question 25

What might cause unexpected bands to appear on a gel?

Accepted Answer

Contamination or non-specific priming

Answer

Using a 9-well comb

Answer

Using 135 mL of buffer

Answer

Running the gel for 25 minutes

Question 26

Which component provides the molecular "ruler" to estimate DNA fragment sizes?

Accepted Answer

DNA Marker

Answer

TBE Buffer

Answer

Agarose Gel

Answer

Loading Dye

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