Question 1

Which of the following is true for Genetic Engineering?

Accepted Answer

It involves making a recombinant DNA made from selected gene of an organism transferred to the DNA of another organism giving the organism an additional trait.

Answer

Wagyu beef, guapple, human insulin-producing bacteria, Flavr-Savr tomatoes, and macapuno coconuts are examples of products of genetic engineering.

Answer

Neither

Answer

Both

Question 2

Which of the following comes first in Genetic Engineering?

Accepted Answer

Cutting the DNA that will serve as source of gene of interest and the DNA that will serve as vector using the same restriction enzymes.

Answer

Combining or ligating the gene of interest and the vector to form the recombinant DNA.

Answer

Looking for cells which have successfully transformed themselves by incorporating the recombinant DNA in their systems.

Answer

Inserting the recombinant DNA into a host cell (usually, bacteria but can be another).

Question 3

Which of the following will allow the recombinant DNA to enter a host cell and become transformed?

Accepted Answer

Both

Answer

Biolistics that uses gene gun firing the rDNA as part of a pellet.

Answer

Heat shock and electroporation that will make target cells to be more competent in accepting rDNA by opening cell membrane pores larger.

Answer

Neither

Question 4

Which of the following makes thousands to millions of copies of DNA from a few copy of DNA?

Accepted Answer

Polymerase chain reaction (PCR) amplification

Answer

DNA recombination

Answer

Classical breeding

Answer

Genetically modified organisms (GMO)

Question 5

Which of the following is an application of recombinant DNA technology or genetic engineering?

Accepted Answer

Both

Answer

Enhancement of present trait like tomatoes expressing better resistance from rotting by inhibiting production of enzymes that make their cell wall degrade.

Answer

Introduction of new traits like bacteria having the ability to produce insulin that is normally for animals like mammals only.

Answer

Neither

Question 6

Why are some experts skeptic about promoting GMO or transgenics?

Accepted Answer

All of the above

Answer

The government policies on GMO must be clear and sufficient first and the people must have informed decision before they are exposed to GMOs.

Answer

Creating an unnaturally enhanced organism may make the natural habitat unprepared for its dominance, hence, may create imbalance once allowed to spread outside the laboratories.

Answer

It is possible that even after rigorous studies, artificially changed DNA still has secrets that might have not been exposed to experts which may cause problems later to consumers or ecosystem.

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