Question 1

1. What method is directly changing the DNA of an organism?

Accepted Answer

Genetic engineering

Answer

Hybridization

Answer

Selective breeding

Answer

Interbreeding

Question 2

Which of the following can cut genes at specific DNA sequences?

Accepted Answer

Restriction enzyme

Answer

Plasmid

Answer

Recombinant DNA

Answer

DNA ligase

Question 3

Which of the following acts like a “glue” that puts together the DNA fragment and the plasmid?

Accepted Answer

DNA ligase

Answer

Vector

Answer

Restriction enzyme

Answer

Host cell

Question 4

Which of the following is the first step in genetic engineering?

Accepted Answer

A DNA fragment that has the gene of interest is taken from a donor cell.

Answer

The DNA fragment that has the gene of interest is inserted into the open plasmid with the help of DNA ligase.

Answer

A. The DNA fragment that has the gene of interest is cut with the restriction enzyme.

Answer

A suitable plasmid is taken from a bacterium.

Question 5

Which of the following is NOT a step in recombinant DNA technology?

Accepted Answer

Utilize a DNA ligase to read the new DNA sequence.

Answer

Cut out a fragment of DNA from a DNA strand.

Answer

Use a restriction enzyme to cut DNA and form sticky ends.

Answer

Add a fragment of DNA from one organism into the DNA of another.

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