Question 1

What is the primary purpose of hybridoma technology in the context of monoclonal antibody production?

Accepted Answer

To produce large quantities of identical antibodies

Answer

To isolate a single type of antibody from blood

Answer

To increase the genetic diversity of antibodies

Answer

To create a variety of different antibody types

Question 2

Which of the following best describes the screening process in hybridoma technology?

Accepted Answer

Identifying hybridomas that produce the desired antibody

Answer

Selecting cells based on their size

Answer

Measuring the overall protein content of cells

Answer

Testing for the rate of cell division

Question 3

What is the main advantage of scaling up hybridoma cell culture?

Accepted Answer

Increased production of monoclonal antibodies

Answer

Reduced time for screening hybridomas

Answer

Improved genetic variability in antibody production

Answer

Decreased cost of raw materials

Question 4

Which method is commonly used to confirm that a hybridoma produces the correct antibody?

Accepted Answer

Enzyme-linked immunosorbent assay (ELISA)

Answer

Flow cytometry

Answer

Western blotting

Answer

Polymerase chain reaction (PCR)

Question 5

What role do myeloma cells play in hybridoma technology?

Accepted Answer

They provide a longer lifespan for the fused cells

Answer

They produce antibodies independently

Answer

They increase genetic diversity among antibodies

Answer

They enhance the immune response

Question 6

During hybridoma technology, what is the purpose of using the selective medium after the cell fusion step?

Accepted Answer

To allow only hybridoma cells to survive

Answer

To promote the growth of myeloma cells only

Answer

To enhance antibody production from all cells

Answer

To increase the mutation rate of the cells

Question 7

What is a key characteristic of monoclonal antibodies produced by hybridoma technology?

Accepted Answer

They are identical molecules produced from a single clone of cells

Answer

They vary in their structure and function

Answer

They can only bind to multiple antigens

Answer

They are produced from multiple sources of cells

Question 8

What is the primary reason for using hybridoma technology over polyclonal antibody production?

Accepted Answer

Consistency and specificity of the antibodies produced

Answer

Greater diversity in antibody types

Answer

Faster production times

Answer

Lower production costs

Question 9

What technique is often used to characterize the binding specificity of the antibodies produced by hybridomas?

Accepted Answer

Immunoassays

Answer

Chromatography

Answer

Microscopy

Answer

Spectroscopy

Question 10

Which of the following factors is critical for the successful large-scale culture of hybridoma cells?

Accepted Answer

Maintaining optimal nutrient levels and environmental conditions

Answer

Avoiding any turbulence during cell culture

Answer

Minimizing the number of cells in culture

Answer

Using only serum-free media

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